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FAM/HEX, lightcyler 480 - (Sep/21/2010 )


I'm using the lighcycler 480 for doing qPCR.
I assume that my primers are labeled with FAM since i only detect amplification when i click on the "Filter comb: 465-510"
When i click on filtercomb 533-580, i dont detect anything at all.
So i assume that if my primers were labeled with HEX, i have to use the filercomb 533-580?




You use labeled primers? What for? Usually probe is labeled or mix contains SYBR which emits in the 465-510 filter combination.
Anyway, there is table with corresponding filters in the Instrument Operators manual on page 56-7, and yes, HEX is in the filter 533-580 combination.