a second small peak to the right to my pricipal peak!!! - (Apr/26/2011 )
i'm doing my PCR tests using a stratagen MX3005P and SYBRgreen.
i'm having a second small peak at different C° whiec are higher than the C° of my desiered peak!!!!
is there any one who can suggest to me the reason why i'm having that and if yes what should i do....
THX a lot.
Unspecific product, try to increase stringency of the reaction, higer Ta or less magnesium.