Measuring of cDNA bands density - (Nov/23/2011 )

Hello.

To my unfortunate I am forced to measure the intensity of bands on agarose gel after basic PCR.
I found the Scion Image programe in internet, I read the manual, I follow all steps, I think I understand how it should work and no result. It doesn`t allow me to plot even two adjacent bands.
The example in the manual is for PAGE and protein bands, which are situated in column, not in a single row, how it is on the agarose gels.
Does anybody know what could be the problem and if there is a simple, efficient and free software for analysis of DNA bands?

Many thanks,

Nephrite

-Nephrit-

I've never used (or even heard of) Scion Image, but for what you want you can use ImageJ. Is free online, and very easy to use.

-almost a doctor-

Thank you very much!

-Nephrit-

yes you can use ImageJ..

Follow the Link to know more about it

http://lifescienceso...s-software.html

-Biouday-