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cDNA sequencing - (Sep/27/2012 )

Hi all,

Just wanted to know that if I plan to use cDNA sequencing to determine the sequence of a particular gene, how can we determine the sequence of the complete length of the cDNA. Doesn't common sequencing have limits of 600-800 bp? So, if my cDNA is say 1600 bp long, how can I determine the sequence of the other 800 bp? Or is it that other methods of sequencing are employed in such cases.

Thanks for your help.


-Ameya P-

The method is called "design additional sequencing primer in the middle of your sequence" ;)


You can also try 5' and 3' RACE.