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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1. Primer dilution - (reply: 7)
2. Calculation help - (reply: 6)
3. standards for qPCR and some other doubts - (reply: 8)
4. Plasma cortisol/corticosterone level analysis using qPCR - (reply: 1)
5. Technical Replicates vs Biological Replicates - (reply: 1)
6. pcr - (reply: 1)
7. Difference in synthesis - (reply: 1)
8. Standardizing cDNA concentration before doing PCR - (reply: 1)
9. Agarose Gel Electrophoresis - (reply: 1)
10. Volume of Primers - (reply: 4)
11. qPCR with different genes - (reply: 5)
12. PCR conditions unknown? - (reply: 10)
13. PCR anomaly in a mid-range dilution of the template - (reply: 2)
14. Reducing contamination in 16s PCR for metagenomic library prer - (reply: 4)
15. 2 peaks for identical sequences in HRM - (reply: 2)
16. PCR using very long oligos !!! - (reply: 4)
17. Housekeeping gene for Real Time PCR when comparing expression of genes across di - (reply: 1)
18. How to optimize a master mix kit? - (reply: 7)
19. pcr primer design - (reply: 6)
20. Please help (primer/ probe final volume calculations) - (reply: 1)
21. Primer design with EcoRI ends - (reply: 2)
22. qPCR normalization - (reply: 4)
23. Reference Gene/HKG- every plate - (reply: 2)
24. qPCR standard for lipase genes - (reply: 2)
25. PCR with overlapping primers only (primer extention) - (reply: 3)
26. Random chuck of nonsense in middle of PCR - (reply: 6)
27. Campylobacter jejuni qPCR targeting VS1 gene - (reply: 2)
28. RT-PCR analysis - (reply: 1)
29. Newbie to qPCR - (reply: 4)
30. real time qPCR standard range plasmid - (reply: 3)