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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1. ABI 7900HT big alarm - (reply: 1)
2. Light genotyping PCR bands - (reply: 4)
3. What is the relation between amplicon size and melting temp? - (reply: 2)
4. Viral isolation kit QIA buffer substitute - (reply: 3)
5. Efficiency curve of primers - (reply: 1)
6. How do determine the reference gene for SMN 2 - (reply: 1)
7. Dengue Virus Serotyping - (reply: 1)
8. gain optimization closest match selected - (reply: 1)
9. Low amount of RNA and cDNa for qPCR - (reply: 1)
10. An odd situation: rfu values & scoring of AFLP fragments in clonally propaga - (reply: 2)
11. How to publish real time PCR results? - (reply: 2)
12. issue with PCR - (reply: 1)
13. RNA degradation from cells embedded in gels - (reply: 4)
14. ITS gene PCR Smears - (reply: 6)
15. Trouble with PCR after cloning - (reply: 1)
16. Problem with cDNA synthesis - (reply: 2)
17. real-time PCR machine error - (reply: 1)
18. what may have gone wrong? - (reply: 21)
19. bands are not discrete in PCR, why? - (reply: 1)
20. Verifying positive during PCR amplification - (reply: 1)
21. how to predict amplicon size? - (reply: 1)
22. Mastermix expiry dates? - (reply: 2)
23. Optimization of real time-PCR - where should I start from? - (reply: 2)
24. Weird PCR results - (reply: 2)
25. Primer design (Real time PCR) - (reply: 2)
26. Exact amplification in negative control??? - (reply: 1)
27. PCR is inconsistent - (reply: 4)
28. Specific primer problem - (reply: 2)
29. How to minimize delta ct value when everything else worked well in qRT PCR - (reply: 1)
30. How to minimize delta ct value when everything else worked well in qRT PCR - (reply: 1)