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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1. The curve of sample one is ideal or not - (reply: 1)
2. REAL TIME PCR WITH TAQMAN PROBE - (reply: 1)
3. Optimal dNTP storage pH - (reply: 1)
4. Recombinant DNA vaccine production - (reply: 9)
5. Amount of cDNA input in RT-PCR - (reply: 1)
6. Housekeeper Genes and Real-Time PCR - (reply: 1)
7. Pre-designed qRT-PCR primers - (reply: 1)
8. Same primers and different melting peaks for two samples (~1C appart) - (reply: 2)
9. Gene expression in colonic tissues using qRT-PCR - (reply: 1)
10. qPCR run w. strong positive NTC signal but very low fluorescence - (reply: 1)
11. Designing primers with his-tag for pET28a+ vector - (reply: 3)
12. making your own qPCR kit! - (reply: 1)
13. Problem with TaqMan qPCR efficiency - (reply: 1)
14. High molecular weight band from my PCR - (reply: 3)
15. relative quantification - things to do before the actual qPCR - (reply: 3)
16. is it how total cDNA should look like? - (reply: 3)
17. testing primers efficiency for rt-PCR - (reply: 1)
18. -RT contamination problem in PCR - (reply: 5)
19. Strange Taqman probe negative fluorescence - (reply: 2)
20. qPCR std curve calculation issue if using PCR product as the std - (reply: 1)
21. qPCR , eDNA and standard curve - (reply: 3)
22. Thermal Cycler for Multiplex PCR - (reply: 3)
23. Problem of melt curve shape for pikoreal real time PCR - (reply: 1)
24. Primer sequences with M and Y - how to order? - (reply: 2)
25. QPCR with genomic DNA from blood - (reply: 2)
26. RT-PCR low efficiency - (reply: 1)
27. emulsion PCR - (reply: 1)
28. qPCR data analysis - (reply: 2)
29. Addition of DNase after RNA extraction - (reply: 4)
30. RT-PCR electrophorasis - (reply: 1)