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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1. Pre-designed qRT-PCR primers - (reply: 1)
2. Same primers and different melting peaks for two samples (~1C appart) - (reply: 2)
3. Gene expression in colonic tissues using qRT-PCR - (reply: 1)
4. qPCR run w. strong positive NTC signal but very low fluorescence - (reply: 1)
5. Designing primers with his-tag for pET28a+ vector - (reply: 2)
6. making your own qPCR kit! - (reply: 1)
7. Problem with TaqMan qPCR efficiency - (reply: 1)
8. High molecular weight band from my PCR - (reply: 3)
9. relative quantification - things to do before the actual qPCR - (reply: 3)
10. is it how total cDNA should look like? - (reply: 3)
11. testing primers efficiency for rt-PCR - (reply: 1)
12. -RT contamination problem in PCR - (reply: 5)
13. Strange Taqman probe negative fluorescence - (reply: 2)
14. qPCR std curve calculation issue if using PCR product as the std - (reply: 1)
15. qPCR , eDNA and standard curve - (reply: 3)
16. Thermal Cycler for Multiplex PCR - (reply: 3)
17. Problem of melt curve shape for pikoreal real time PCR - (reply: 1)
18. Primer sequences with M and Y - how to order? - (reply: 2)
19. QPCR with genomic DNA from blood - (reply: 2)
20. RT-PCR low efficiency - (reply: 1)
21. emulsion PCR - (reply: 1)
22. qPCR data analysis - (reply: 2)
23. Addition of DNase after RNA extraction - (reply: 4)
24. RT-PCR electrophorasis - (reply: 1)
25. PCR contamination - (reply: 6)
26. RNA agarose gel blurry fuzzy. RNase contaminaton? - (reply: 2)
27. cDNA serial dilution trouble in qPCR: help needed - (reply: 2)
28. High background fluorescence detected before starting PCR - (reply: 3)
29. Real-time pcr - (reply: 1)
30. Using qPCR machine for simple PCR, is it possible? - (reply: 3)