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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1. Verifying positive during PCR amplification - (reply: 1)
2. how to predict amplicon size? - (reply: 1)
3. Mastermix expiry dates? - (reply: 1)
4. Optimization of real time-PCR - where should I start from? - (reply: 2)
5. Weird PCR results - (reply: 2)
6. Primer design (Real time PCR) - (reply: 2)
7. Exact amplification in negative control??? - (reply: 1)
8. PCR is inconsistent - (reply: 4)
9. Specific primer problem - (reply: 2)
10. How to minimize delta ct value when everything else worked well in qRT PCR - (reply: 1)
11. How to minimize delta ct value when everything else worked well in qRT PCR - (reply: 1)
12. RAPD PCR - (reply: 2)
13. Instrument blocklower error - (reply: 2)
14. Long pcr frustration and problem.. all help is Much appreciated - (reply: 3)
15. calculating ramp rate on thermocycler - urgent - (reply: 1)
16. How to check if my primers were degraded? - (reply: 2)
17. problem with PCR amplification of cDNA - (reply: 4)
18. Funny shaped PCR curve - (reply: 1)
19. How clean do I need my DNA to be? - (reply: 1)
20. Problematic DNA Extraction - (reply: 1)
21. Length of amplicon for RT real time PCR - (reply: 2)
22. The curve of sample one is ideal or not - (reply: 1)
23. REAL TIME PCR WITH TAQMAN PROBE - (reply: 1)
24. Optimal dNTP storage pH - (reply: 1)
25. Recombinant DNA vaccine production - (reply: 9)
26. Amount of cDNA input in RT-PCR - (reply: 1)
27. Housekeeper Genes and Real-Time PCR - (reply: 1)
28. Pre-designed qRT-PCR primers - (reply: 1)
29. Same primers and different melting peaks for two samples (~1C appart) - (reply: 2)
30. Gene expression in colonic tissues using qRT-PCR - (reply: 1)