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New Forum Archives (2009-)
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PCR--RT-PCR-and-Real-Time-PCR
1.
ABI 7900HT big alarm -
(reply: 1)
2.
Light genotyping PCR bands -
(reply: 4)
3.
What is the relation between amplicon size and melting temp? -
(reply: 2)
4.
Viral isolation kit QIA buffer substitute -
(reply: 3)
5.
Efficiency curve of primers -
(reply: 1)
6.
How do determine the reference gene for SMN 2 -
(reply: 1)
7.
Dengue Virus Serotyping -
(reply: 1)
8.
gain optimization closest match selected -
(reply: 1)
9.
Low amount of RNA and cDNa for qPCR -
(reply: 1)
10.
An odd situation: rfu values & scoring of AFLP fragments in clonally propaga -
(reply: 2)
11.
How to publish real time PCR results? -
(reply: 2)
12.
issue with PCR -
(reply: 1)
13.
RNA degradation from cells embedded in gels -
(reply: 4)
14.
ITS gene PCR Smears -
(reply: 6)
15.
Trouble with PCR after cloning -
(reply: 1)
16.
Problem with cDNA synthesis -
(reply: 2)
17.
real-time PCR machine error -
(reply: 1)
18.
what may have gone wrong? -
(reply: 21)
19.
bands are not discrete in PCR, why? -
(reply: 1)
20.
Verifying positive during PCR amplification -
(reply: 1)
21.
how to predict amplicon size? -
(reply: 1)
22.
Mastermix expiry dates? -
(reply: 2)
23.
Optimization of real time-PCR - where should I start from? -
(reply: 2)
24.
Weird PCR results -
(reply: 2)
25.
Primer design (Real time PCR) -
(reply: 2)
26.
Exact amplification in negative control??? -
(reply: 1)
27.
PCR is inconsistent -
(reply: 4)
28.
Specific primer problem -
(reply: 2)
29.
How to minimize delta ct value when everything else worked well in qRT PCR -
(reply: 1)
30.
How to minimize delta ct value when everything else worked well in qRT PCR -
(reply: 1)
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