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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1. High molecular weight band from my PCR - (reply: 3)
2. relative quantification - things to do before the actual qPCR - (reply: 3)
3. is it how total cDNA should look like? - (reply: 3)
4. testing primers efficiency for rt-PCR - (reply: 1)
5. -RT contamination problem in PCR - (reply: 5)
6. Strange Taqman probe negative fluorescence - (reply: 1)
7. qPCR std curve calculation issue if using PCR product as the std - (reply: 1)
8. qPCR , eDNA and standard curve - (reply: 3)
9. Thermal Cycler for Multiplex PCR - (reply: 3)
10. Problem of melt curve shape for pikoreal real time PCR - (reply: 1)
11. Primer sequences with M and Y - how to order? - (reply: 2)
12. QPCR with genomic DNA from blood - (reply: 2)
13. RT-PCR low efficiency - (reply: 1)
14. emulsion PCR - (reply: 1)
15. qPCR data analysis - (reply: 2)
16. Addition of DNase after RNA extraction - (reply: 4)
17. RT-PCR electrophorasis - (reply: 1)
18. PCR contamination - (reply: 6)
19. RNA agarose gel blurry fuzzy. RNase contaminaton? - (reply: 2)
20. cDNA serial dilution trouble in qPCR: help needed - (reply: 2)
21. High background fluorescence detected before starting PCR - (reply: 3)
22. Real-time pcr - (reply: 1)
23. Using qPCR machine for simple PCR, is it possible? - (reply: 3)
24. What could be the possible reasons for a RT-PCR experiment that was working fine - (reply: 4)
25. Combining standard error of normalised fold change values - Qbase method - (reply: 2)
26. What cycle is best for analysing data? - (reply: 2)
27. Mineral oil quality in PCR tubes - (reply: 5)
28. Internal control for absolute quantification - (reply: 3)
29. Diagnostic PCR troubleshooting - (reply: 2)
30. QPCR replicates issue - (reply: 1)