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1. How clean do I need my DNA to be? - (reply: 1)
2. Problematic DNA Extraction - (reply: 1)
3. Length of amplicon for RT real time PCR - (reply: 2)
4. The curve of sample one is ideal or not - (reply: 1)
5. REAL TIME PCR WITH TAQMAN PROBE - (reply: 1)
6. Optimal dNTP storage pH - (reply: 1)
7. Recombinant DNA vaccine production - (reply: 9)
8. Amount of cDNA input in RT-PCR - (reply: 1)
9. Housekeeper Genes and Real-Time PCR - (reply: 1)
10. Pre-designed qRT-PCR primers - (reply: 1)
11. Same primers and different melting peaks for two samples (~1°C appart) - (reply: 2)
12. Gene expression in colonic tissues using qRT-PCR - (reply: 1)
13. qPCR run w. strong positive NTC signal but very low fluorescence - (reply: 1)
14. Designing primers with his-tag for pET28a+ vector - (reply: 3)
15. making your own qPCR kit! - (reply: 1)
16. Problem with TaqMan qPCR efficiency - (reply: 1)
17. High molecular weight band from my PCR - (reply: 3)
18. relative quantification - things to do before the actual qPCR - (reply: 3)
19. is it how total cDNA should look like? - (reply: 3)
20. testing primers efficiency for rt-PCR - (reply: 1)
21. -RT contamination problem in PCR - (reply: 5)
22. Strange Taqman probe negative fluorescence - (reply: 2)
23. qPCR std curve calculation issue if using PCR product as the std - (reply: 1)
24. qPCR , eDNA and standard curve - (reply: 3)
25. Thermal Cycler for Multiplex PCR - (reply: 3)
26. Problem of melt curve shape for pikoreal real time PCR - (reply: 1)
27. Primer sequences with M and Y - how to order? - (reply: 2)
28. QPCR with genomic DNA from blood - (reply: 2)
29. RT-PCR low efficiency - (reply: 1)
30. emulsion PCR - (reply: 1)
2. Problematic DNA Extraction - (reply: 1)
3. Length of amplicon for RT real time PCR - (reply: 2)
4. The curve of sample one is ideal or not - (reply: 1)
5. REAL TIME PCR WITH TAQMAN PROBE - (reply: 1)
6. Optimal dNTP storage pH - (reply: 1)
7. Recombinant DNA vaccine production - (reply: 9)
8. Amount of cDNA input in RT-PCR - (reply: 1)
9. Housekeeper Genes and Real-Time PCR - (reply: 1)
10. Pre-designed qRT-PCR primers - (reply: 1)
11. Same primers and different melting peaks for two samples (~1°C appart) - (reply: 2)
12. Gene expression in colonic tissues using qRT-PCR - (reply: 1)
13. qPCR run w. strong positive NTC signal but very low fluorescence - (reply: 1)
14. Designing primers with his-tag for pET28a+ vector - (reply: 3)
15. making your own qPCR kit! - (reply: 1)
16. Problem with TaqMan qPCR efficiency - (reply: 1)
17. High molecular weight band from my PCR - (reply: 3)
18. relative quantification - things to do before the actual qPCR - (reply: 3)
19. is it how total cDNA should look like? - (reply: 3)
20. testing primers efficiency for rt-PCR - (reply: 1)
21. -RT contamination problem in PCR - (reply: 5)
22. Strange Taqman probe negative fluorescence - (reply: 2)
23. qPCR std curve calculation issue if using PCR product as the std - (reply: 1)
24. qPCR , eDNA and standard curve - (reply: 3)
25. Thermal Cycler for Multiplex PCR - (reply: 3)
26. Problem of melt curve shape for pikoreal real time PCR - (reply: 1)
27. Primer sequences with M and Y - how to order? - (reply: 2)
28. QPCR with genomic DNA from blood - (reply: 2)
29. RT-PCR low efficiency - (reply: 1)
30. emulsion PCR - (reply: 1)