Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1. Problem with TaqMan qPCR efficiency - (reply: 1)
2. High molecular weight band from my PCR - (reply: 3)
3. relative quantification - things to do before the actual qPCR - (reply: 3)
4. is it how total cDNA should look like? - (reply: 3)
5. testing primers efficiency for rt-PCR - (reply: 1)
6. -RT contamination problem in PCR - (reply: 5)
7. Strange Taqman probe negative fluorescence - (reply: 2)
8. qPCR std curve calculation issue if using PCR product as the std - (reply: 1)
9. qPCR , eDNA and standard curve - (reply: 3)
10. Thermal Cycler for Multiplex PCR - (reply: 3)
11. Problem of melt curve shape for pikoreal real time PCR - (reply: 1)
12. Primer sequences with M and Y - how to order? - (reply: 2)
13. QPCR with genomic DNA from blood - (reply: 2)
14. RT-PCR low efficiency - (reply: 1)
15. emulsion PCR - (reply: 1)
16. qPCR data analysis - (reply: 2)
17. Addition of DNase after RNA extraction - (reply: 4)
18. RT-PCR electrophorasis - (reply: 1)
19. PCR contamination - (reply: 6)
20. RNA agarose gel blurry fuzzy. RNase contaminaton? - (reply: 2)
21. cDNA serial dilution trouble in qPCR: help needed - (reply: 2)
22. High background fluorescence detected before starting PCR - (reply: 3)
23. Real-time pcr - (reply: 1)
24. Using qPCR machine for simple PCR, is it possible? - (reply: 3)
25. What could be the possible reasons for a RT-PCR experiment that was working fine - (reply: 4)
26. Combining standard error of normalised fold change values - Qbase method - (reply: 2)
27. What cycle is best for analysing data? - (reply: 2)
28. Mineral oil quality in PCR tubes - (reply: 5)
29. Internal control for absolute quantification - (reply: 3)
30. Diagnostic PCR troubleshooting - (reply: 2)