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1. How to prepare serial dilutions of RNA or cDNA - (reply: 3)
2. Are these primer products good enough for qPCR? - (reply: 3)
3. mtDNA to nDNA Ratio gDNA - qPCR - (reply: 1)
4. Can't get PCR with large overhang primers to work - (reply: 8)
5. Question about pipet tips for PCR and rtPCR - (reply: 4)
6. Taqman Probe sequence problem - (reply: 4)
7. UNG in PCR - (reply: 1)
8. Cause of random samples failing PCR? - (reply: 2)
9. Template DNA for PCR- Concrentration or Volume?? - (reply: 1)
10. RNA extraction - (reply: 1)
11. Normalization of Ct of interest to a reference Ct - (reply: 1)
12. urgent: need help with qPCR statistics - (reply: 2)
13. A question for Reverse transcription experts - (reply: 5)
14. will mRNA from different cell line lead to loss of expected product in RT PCR? - (reply: 2)
15. PCR amplification with new restriction sites troubleshooting - (reply: 2)
16. using cell line to generate standard curve - (reply: 5)
17. 3' RACE creates too big band doesn't leave well - (reply: 1)
18. RT-PCR product- no band - (reply: 4)
19. Normalization factor - (reply: 3)
20. Understanding RACE PCR - (reply: 1)
21. SYBR Select Master Mix for CFX from Invitrogen - Storage - (reply: 4)
22. Dye for qPCR - (reply: 3)
23. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
24. Wierd Bands after PCR....Confused - (reply: 9)
25. PCR with Plasmid recovered from filter paper - (reply: 6)
26. PCR amplified product size - (reply: 5)
27. Opinion: What fold change is actually considered meaningful - (reply: 2)
28. Buffers RNase decontamination - (reply: 1)
29. the storage time for primers - (reply: 9)
30. Annealing temperature for PCR - (reply: 8)
2. Are these primer products good enough for qPCR? - (reply: 3)
3. mtDNA to nDNA Ratio gDNA - qPCR - (reply: 1)
4. Can't get PCR with large overhang primers to work - (reply: 8)
5. Question about pipet tips for PCR and rtPCR - (reply: 4)
6. Taqman Probe sequence problem - (reply: 4)
7. UNG in PCR - (reply: 1)
8. Cause of random samples failing PCR? - (reply: 2)
9. Template DNA for PCR- Concrentration or Volume?? - (reply: 1)
10. RNA extraction - (reply: 1)
11. Normalization of Ct of interest to a reference Ct - (reply: 1)
12. urgent: need help with qPCR statistics - (reply: 2)
13. A question for Reverse transcription experts - (reply: 5)
14. will mRNA from different cell line lead to loss of expected product in RT PCR? - (reply: 2)
15. PCR amplification with new restriction sites troubleshooting - (reply: 2)
16. using cell line to generate standard curve - (reply: 5)
17. 3' RACE creates too big band doesn't leave well - (reply: 1)
18. RT-PCR product- no band - (reply: 4)
19. Normalization factor - (reply: 3)
20. Understanding RACE PCR - (reply: 1)
21. SYBR Select Master Mix for CFX from Invitrogen - Storage - (reply: 4)
22. Dye for qPCR - (reply: 3)
23. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
24. Wierd Bands after PCR....Confused - (reply: 9)
25. PCR with Plasmid recovered from filter paper - (reply: 6)
26. PCR amplified product size - (reply: 5)
27. Opinion: What fold change is actually considered meaningful - (reply: 2)
28. Buffers RNase decontamination - (reply: 1)
29. the storage time for primers - (reply: 9)
30. Annealing temperature for PCR - (reply: 8)