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1. Same primers and different melting peaks for two samples (~1°C appart) - (reply: 2)
2. Gene expression in colonic tissues using qRT-PCR - (reply: 1)
3. qPCR run w. strong positive NTC signal but very low fluorescence - (reply: 1)
4. Designing primers with his-tag for pET28a+ vector - (reply: 2)
5. making your own qPCR kit! - (reply: 1)
6. Problem with TaqMan qPCR efficiency - (reply: 1)
7. High molecular weight band from my PCR - (reply: 3)
8. relative quantification - things to do before the actual qPCR - (reply: 3)
9. is it how total cDNA should look like? - (reply: 3)
10. testing primers efficiency for rt-PCR - (reply: 1)
11. -RT contamination problem in PCR - (reply: 5)
12. Strange Taqman probe negative fluorescence - (reply: 2)
13. qPCR std curve calculation issue if using PCR product as the std - (reply: 1)
14. qPCR , eDNA and standard curve - (reply: 3)
15. Thermal Cycler for Multiplex PCR - (reply: 3)
16. Problem of melt curve shape for pikoreal real time PCR - (reply: 1)
17. Primer sequences with M and Y - how to order? - (reply: 2)
18. QPCR with genomic DNA from blood - (reply: 2)
19. RT-PCR low efficiency - (reply: 1)
20. emulsion PCR - (reply: 1)
21. qPCR data analysis - (reply: 2)
22. Addition of DNase after RNA extraction - (reply: 4)
23. RT-PCR electrophorasis - (reply: 1)
24. PCR contamination - (reply: 6)
25. RNA agarose gel blurry fuzzy. RNase contaminaton? - (reply: 2)
26. cDNA serial dilution trouble in qPCR: help needed - (reply: 2)
27. High background fluorescence detected before starting PCR - (reply: 3)
28. Real-time pcr - (reply: 1)
29. Using qPCR machine for simple PCR, is it possible? - (reply: 3)
30. What could be the possible reasons for a RT-PCR experiment that was working fine - (reply: 4)
2. Gene expression in colonic tissues using qRT-PCR - (reply: 1)
3. qPCR run w. strong positive NTC signal but very low fluorescence - (reply: 1)
4. Designing primers with his-tag for pET28a+ vector - (reply: 2)
5. making your own qPCR kit! - (reply: 1)
6. Problem with TaqMan qPCR efficiency - (reply: 1)
7. High molecular weight band from my PCR - (reply: 3)
8. relative quantification - things to do before the actual qPCR - (reply: 3)
9. is it how total cDNA should look like? - (reply: 3)
10. testing primers efficiency for rt-PCR - (reply: 1)
11. -RT contamination problem in PCR - (reply: 5)
12. Strange Taqman probe negative fluorescence - (reply: 2)
13. qPCR std curve calculation issue if using PCR product as the std - (reply: 1)
14. qPCR , eDNA and standard curve - (reply: 3)
15. Thermal Cycler for Multiplex PCR - (reply: 3)
16. Problem of melt curve shape for pikoreal real time PCR - (reply: 1)
17. Primer sequences with M and Y - how to order? - (reply: 2)
18. QPCR with genomic DNA from blood - (reply: 2)
19. RT-PCR low efficiency - (reply: 1)
20. emulsion PCR - (reply: 1)
21. qPCR data analysis - (reply: 2)
22. Addition of DNase after RNA extraction - (reply: 4)
23. RT-PCR electrophorasis - (reply: 1)
24. PCR contamination - (reply: 6)
25. RNA agarose gel blurry fuzzy. RNase contaminaton? - (reply: 2)
26. cDNA serial dilution trouble in qPCR: help needed - (reply: 2)
27. High background fluorescence detected before starting PCR - (reply: 3)
28. Real-time pcr - (reply: 1)
29. Using qPCR machine for simple PCR, is it possible? - (reply: 3)
30. What could be the possible reasons for a RT-PCR experiment that was working fine - (reply: 4)