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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1. Recombinant DNA vaccine production - (reply: 9)
2. Amount of cDNA input in RT-PCR - (reply: 1)
3. Housekeeper Genes and Real-Time PCR - (reply: 1)
4. Pre-designed qRT-PCR primers - (reply: 1)
5. Same primers and different melting peaks for two samples (~1C appart) - (reply: 2)
6. Gene expression in colonic tissues using qRT-PCR - (reply: 1)
7. qPCR run w. strong positive NTC signal but very low fluorescence - (reply: 1)
8. Designing primers with his-tag for pET28a+ vector - (reply: 3)
9. making your own qPCR kit! - (reply: 1)
10. Problem with TaqMan qPCR efficiency - (reply: 1)
11. High molecular weight band from my PCR - (reply: 3)
12. relative quantification - things to do before the actual qPCR - (reply: 3)
13. is it how total cDNA should look like? - (reply: 3)
14. testing primers efficiency for rt-PCR - (reply: 1)
15. -RT contamination problem in PCR - (reply: 5)
16. Strange Taqman probe negative fluorescence - (reply: 2)
17. qPCR std curve calculation issue if using PCR product as the std - (reply: 1)
18. qPCR , eDNA and standard curve - (reply: 3)
19. Thermal Cycler for Multiplex PCR - (reply: 3)
20. Problem of melt curve shape for pikoreal real time PCR - (reply: 1)
21. Primer sequences with M and Y - how to order? - (reply: 2)
22. QPCR with genomic DNA from blood - (reply: 2)
23. RT-PCR low efficiency - (reply: 1)
24. emulsion PCR - (reply: 1)
25. qPCR data analysis - (reply: 2)
26. Addition of DNase after RNA extraction - (reply: 4)
27. RT-PCR electrophorasis - (reply: 1)
28. PCR contamination - (reply: 6)
29. RNA agarose gel blurry fuzzy. RNase contaminaton? - (reply: 2)
30. cDNA serial dilution trouble in qPCR: help needed - (reply: 2)