Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1. Housekeeper Genes and Real-Time PCR - (reply: 1)
2. Pre-designed qRT-PCR primers - (reply: 1)
3. Same primers and different melting peaks for two samples (~1C appart) - (reply: 2)
4. Gene expression in colonic tissues using qRT-PCR - (reply: 1)
5. qPCR run w. strong positive NTC signal but very low fluorescence - (reply: 1)
6. Designing primers with his-tag for pET28a+ vector - (reply: 2)
7. making your own qPCR kit! - (reply: 1)
8. Problem with TaqMan qPCR efficiency - (reply: 1)
9. High molecular weight band from my PCR - (reply: 3)
10. relative quantification - things to do before the actual qPCR - (reply: 3)
11. is it how total cDNA should look like? - (reply: 3)
12. testing primers efficiency for rt-PCR - (reply: 1)
13. -RT contamination problem in PCR - (reply: 5)
14. Strange Taqman probe negative fluorescence - (reply: 2)
15. qPCR std curve calculation issue if using PCR product as the std - (reply: 1)
16. qPCR , eDNA and standard curve - (reply: 3)
17. Thermal Cycler for Multiplex PCR - (reply: 3)
18. Problem of melt curve shape for pikoreal real time PCR - (reply: 1)
19. Primer sequences with M and Y - how to order? - (reply: 2)
20. QPCR with genomic DNA from blood - (reply: 2)
21. RT-PCR low efficiency - (reply: 1)
22. emulsion PCR - (reply: 1)
23. qPCR data analysis - (reply: 2)
24. Addition of DNase after RNA extraction - (reply: 4)
25. RT-PCR electrophorasis - (reply: 1)
26. PCR contamination - (reply: 6)
27. RNA agarose gel blurry fuzzy. RNase contaminaton? - (reply: 2)
28. cDNA serial dilution trouble in qPCR: help needed - (reply: 2)
29. High background fluorescence detected before starting PCR - (reply: 3)
30. Real-time pcr - (reply: 1)