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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1. need help setting up reverse transcriptase qPCR - (reply: 3)
2. Problems regarding amplification of my gene - (reply: 3)
3. Overlapping PCR, need help - (reply: 5)
4. How does RT-PCR work? - (reply: 5)
5. Denatured plasmid for pcr - (reply: 1)
6. Possible primer dimer problem..Need solution!!! - (reply: 2)
7. When to dilute samples for qPCR - (reply: 2)
8. Some info. on PCR products stability w/respect to shipping - (reply: 3)
9. 3' RACE troubles - (reply: 1)
10. haeting RNA before electrophoresis - (reply: 1)
11. PCR Products not run well in the GEL - (reply: 1)
12. Qubit Nanodrop Quantifications - (reply: 1)
13. Decontaminating DNA - Effective Means? - (reply: 4)
14. Consistancy issue with PCR block - (reply: 1)
15. Queries regarding PCR - (reply: 3)
16. Identification of Listeria monocytogenes - (reply: 1)
17. Calculation of Taq Polymerase - (reply: 3)
18. Best Taq for colony PCR? - (reply: 5)
19. Gene expression varying over time course when it shouldn't (qRT-PCR) - (reply: 6)
20. How to test my milleq water in q PCR about contaminants - (reply: 3)
21. different amount of cDNA in qRT-PCR for target and reference genes - (reply: 3)
22. real time PCR primer design - (reply: 1)
23. Plasmid Linearization Problems - (reply: 9)
24. Plasmid calculation question (Standard Curve Quantification) - (reply: 6)
25. Increasing RNA yield from very small tissues - (reply: 3)
26. Degenerated Primers and their problems - (reply: 1)
27. Interpreting primer BLAST scores for self-complementary - (reply: 5)
28. Testing primers on 'unknown' tissue - (reply: 1)
29. Second Hand Roche Lightcycler II maching - to buy or not to buy - (reply: 1)
30. nested PCR for low viral load- HBV patient sample - (reply: 2)