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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1. RNA degradation from cells embedded in gels - (reply: 4)
2. ITS gene PCR Smears - (reply: 6)
3. Trouble with PCR after cloning - (reply: 1)
4. Problem with cDNA synthesis - (reply: 2)
5. real-time PCR machine error - (reply: 1)
6. what may have gone wrong? - (reply: 21)
7. bands are not discrete in PCR, why? - (reply: 1)
8. Verifying positive during PCR amplification - (reply: 1)
9. how to predict amplicon size? - (reply: 1)
10. Mastermix expiry dates? - (reply: 2)
11. Optimization of real time-PCR - where should I start from? - (reply: 2)
12. Weird PCR results - (reply: 2)
13. Primer design (Real time PCR) - (reply: 2)
14. Exact amplification in negative control??? - (reply: 1)
15. PCR is inconsistent - (reply: 4)
16. Specific primer problem - (reply: 2)
17. How to minimize delta ct value when everything else worked well in qRT PCR - (reply: 1)
18. How to minimize delta ct value when everything else worked well in qRT PCR - (reply: 1)
19. RAPD PCR - (reply: 2)
20. Instrument blocklower error - (reply: 2)
21. Long pcr frustration and problem.. all help is Much appreciated - (reply: 3)
22. calculating ramp rate on thermocycler - urgent - (reply: 1)
23. How to check if my primers were degraded? - (reply: 2)
24. problem with PCR amplification of cDNA - (reply: 4)
25. Funny shaped PCR curve - (reply: 1)
26. How clean do I need my DNA to be? - (reply: 1)
27. Problematic DNA Extraction - (reply: 1)
28. Length of amplicon for RT real time PCR - (reply: 2)
29. The curve of sample one is ideal or not - (reply: 1)
30. REAL TIME PCR WITH TAQMAN PROBE - (reply: 1)