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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1. How to minimize delta ct value when everything else worked well in qRT PCR - (reply: 1)
2. How to minimize delta ct value when everything else worked well in qRT PCR - (reply: 1)
3. RAPD PCR - (reply: 2)
4. Instrument blocklower error - (reply: 2)
5. Long pcr frustration and problem.. all help is Much appreciated - (reply: 3)
6. calculating ramp rate on thermocycler - urgent - (reply: 1)
7. How to check if my primers were degraded? - (reply: 2)
8. problem with PCR amplification of cDNA - (reply: 4)
9. Funny shaped PCR curve - (reply: 1)
10. How clean do I need my DNA to be? - (reply: 1)
11. Problematic DNA Extraction - (reply: 1)
12. Length of amplicon for RT real time PCR - (reply: 2)
13. The curve of sample one is ideal or not - (reply: 1)
14. REAL TIME PCR WITH TAQMAN PROBE - (reply: 1)
15. Optimal dNTP storage pH - (reply: 1)
16. Recombinant DNA vaccine production - (reply: 9)
17. Amount of cDNA input in RT-PCR - (reply: 1)
18. Housekeeper Genes and Real-Time PCR - (reply: 1)
19. Pre-designed qRT-PCR primers - (reply: 1)
20. Same primers and different melting peaks for two samples (~1C appart) - (reply: 2)
21. Gene expression in colonic tissues using qRT-PCR - (reply: 1)
22. qPCR run w. strong positive NTC signal but very low fluorescence - (reply: 1)
23. Designing primers with his-tag for pET28a+ vector - (reply: 3)
24. making your own qPCR kit! - (reply: 1)
25. Problem with TaqMan qPCR efficiency - (reply: 1)
26. High molecular weight band from my PCR - (reply: 3)
27. relative quantification - things to do before the actual qPCR - (reply: 3)
28. is it how total cDNA should look like? - (reply: 3)
29. testing primers efficiency for rt-PCR - (reply: 1)
30. -RT contamination problem in PCR - (reply: 5)