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1. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 3)
2. LightCycler 480 Melting Curve Assay - (reply: 1)
3. Problems with two step RT-PCR - (reply: 1)
4. Spliced and unspliced form of a gene - (reply: 3)
5. primer dilutions - (reply: 4)
6. Genotyping PCR primers - (reply: 1)
7. How to compare these results? Please comment. - (reply: 5)
8. Primers too dilute - (reply: 1)
9. Amplification curves jagged - (reply: 1)
10. ddCT issue: no endogenous expression in control. - (reply: 4)
11. Convering 1:5 serial dilutions to logarithm scale for efficiency curves - (reply: 3)
12. No PCR product in site-directed mutagenesis - (reply: 4)
13. Giardia/Crypto real time PCR late amplification - (reply: 1)
14. How to dilute the template DNA for PCR - (reply: 7)
15. Desired length of overlap region for OE-PCR - (reply: 3)
16. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
17. Need help for PCR - (reply: 5)
18. scorpion probe - (reply: 8)
19. Too low Ct value for reference gene !!! - (reply: 3)
20. unespecific band PCR from mouse genomic DNA - (reply: 9)
21. Help with primer design through PRIMER3 - (reply: 4)
22. Help with unknown band in restriction digest - (reply: 1)
23. how to chose right subsequent for her2(erbb2)gene ? - (reply: 1)
24. 454 sequencing - (reply: 2)
25. Preference for larger of 2 bands? - (reply: 2)
26. Tm of primers - prediction and verification - (reply: 1)
27. acceptable variation for biological replicate - (reply: 2)
28. RT-PCR contamination in negative control. - (reply: 1)
29. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
30. Technical problem with ABI7300 - (reply: 1)
2. LightCycler 480 Melting Curve Assay - (reply: 1)
3. Problems with two step RT-PCR - (reply: 1)
4. Spliced and unspliced form of a gene - (reply: 3)
5. primer dilutions - (reply: 4)
6. Genotyping PCR primers - (reply: 1)
7. How to compare these results? Please comment. - (reply: 5)
8. Primers too dilute - (reply: 1)
9. Amplification curves jagged - (reply: 1)
10. ddCT issue: no endogenous expression in control. - (reply: 4)
11. Convering 1:5 serial dilutions to logarithm scale for efficiency curves - (reply: 3)
12. No PCR product in site-directed mutagenesis - (reply: 4)
13. Giardia/Crypto real time PCR late amplification - (reply: 1)
14. How to dilute the template DNA for PCR - (reply: 7)
15. Desired length of overlap region for OE-PCR - (reply: 3)
16. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
17. Need help for PCR - (reply: 5)
18. scorpion probe - (reply: 8)
19. Too low Ct value for reference gene !!! - (reply: 3)
20. unespecific band PCR from mouse genomic DNA - (reply: 9)
21. Help with primer design through PRIMER3 - (reply: 4)
22. Help with unknown band in restriction digest - (reply: 1)
23. how to chose right subsequent for her2(erbb2)gene ? - (reply: 1)
24. 454 sequencing - (reply: 2)
25. Preference for larger of 2 bands? - (reply: 2)
26. Tm of primers - prediction and verification - (reply: 1)
27. acceptable variation for biological replicate - (reply: 2)
28. RT-PCR contamination in negative control. - (reply: 1)
29. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
30. Technical problem with ABI7300 - (reply: 1)