|
1. COLD-PCR (Dissociation Curve) via qPCR - (reply: 2)
2. how to hasten real-time PCR amplifications - (reply: 2)
3. Please please help me with my Phusion PCR. - (reply: 5)
4. Nanodrop vs Picogreen to quantify post-PCR product - (reply: 2)
5. Need no RT control for all genes? - (reply: 1)
6. Different annealing temp between HKG and target gene - (reply: 2)
7. Multiple melt curves for primer in a sample that does not express gene - (reply: 6)
8. help in long pcr - (reply: 1)
9. Reselling a supermix/enzyme in a commercial diagnostic kit - (reply: 2)
10. Small yield of RNA after on column DNAs digestion , Why - (reply: 3)
11. PCR inhibitor in template DNA - (reply: 3)
12. Why when disigning primers for RTPCR, you need to chose a region where introns a - (reply: 1)
13. Problem with Real-time PCR results analysis - (reply: 1)
14. Rotor-Gene 6000 data analysis - (reply: 1)
15. PCR from protozoa DNA - (reply: 3)
16. Ligation-mediated PCR: Vent Polymerase, Why? - (reply: 6)
17. tool for comparing many primers pairs - (reply: 4)
18. PCR that leads to protein synthesis - (reply: 18)
19. Real-time PCR does not work, while the conventional PCR (with same conditions an - (reply: 3)
20. Use of DMSO in General PCR - (reply: 1)
21. PCR product size confusion - (reply: 3)
22. Concentration specification in PCR - (reply: 3)
23. help us understand the run - (reply: 1)
24. Different MOI in comparison experiment - (reply: 3)
25. How to determine the size of gene to amplify? - (reply: 1)
26. Guanidine isothiocyanate in PCR - (reply: 1)
27. Untreated samples negative, how to analyze fold change? - (reply: 1)
28. Primers have worked well but now getting primer dimers? - (reply: 2)
29. I cannot design primers on exon-exon junction - (reply: 2)
30. DNA Quantification of PCR Products - (reply: 2)
2. how to hasten real-time PCR amplifications - (reply: 2)
3. Please please help me with my Phusion PCR. - (reply: 5)
4. Nanodrop vs Picogreen to quantify post-PCR product - (reply: 2)
5. Need no RT control for all genes? - (reply: 1)
6. Different annealing temp between HKG and target gene - (reply: 2)
7. Multiple melt curves for primer in a sample that does not express gene - (reply: 6)
8. help in long pcr - (reply: 1)
9. Reselling a supermix/enzyme in a commercial diagnostic kit - (reply: 2)
10. Small yield of RNA after on column DNAs digestion , Why - (reply: 3)
11. PCR inhibitor in template DNA - (reply: 3)
12. Why when disigning primers for RTPCR, you need to chose a region where introns a - (reply: 1)
13. Problem with Real-time PCR results analysis - (reply: 1)
14. Rotor-Gene 6000 data analysis - (reply: 1)
15. PCR from protozoa DNA - (reply: 3)
16. Ligation-mediated PCR: Vent Polymerase, Why? - (reply: 6)
17. tool for comparing many primers pairs - (reply: 4)
18. PCR that leads to protein synthesis - (reply: 18)
19. Real-time PCR does not work, while the conventional PCR (with same conditions an - (reply: 3)
20. Use of DMSO in General PCR - (reply: 1)
21. PCR product size confusion - (reply: 3)
22. Concentration specification in PCR - (reply: 3)
23. help us understand the run - (reply: 1)
24. Different MOI in comparison experiment - (reply: 3)
25. How to determine the size of gene to amplify? - (reply: 1)
26. Guanidine isothiocyanate in PCR - (reply: 1)
27. Untreated samples negative, how to analyze fold change? - (reply: 1)
28. Primers have worked well but now getting primer dimers? - (reply: 2)
29. I cannot design primers on exon-exon junction - (reply: 2)
30. DNA Quantification of PCR Products - (reply: 2)