Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1. How do determine the reference gene for SMN 2 - (reply: 1)
2. Dengue Virus Serotyping - (reply: 1)
3. Low amount of RNA and cDNa for qPCR - (reply: 1)
4. An odd situation: rfu values & scoring of AFLP fragments in clonally propaga - (reply: 1)
5. How to publish real time PCR results? - (reply: 2)
6. issue with PCR - (reply: 1)
7. RNA degradation from cells embedded in gels - (reply: 4)
8. ITS gene PCR Smears - (reply: 6)
9. Trouble with PCR after cloning - (reply: 1)
10. Problem with cDNA synthesis - (reply: 2)
11. real-time PCR machine error - (reply: 1)
12. what may have gone wrong? - (reply: 21)
13. bands are not discrete in PCR, why? - (reply: 1)
14. Verifying positive during PCR amplification - (reply: 1)
15. how to predict amplicon size? - (reply: 1)
16. Mastermix expiry dates? - (reply: 2)
17. Optimization of real time-PCR - where should I start from? - (reply: 2)
18. Weird PCR results - (reply: 2)
19. Primer design (Real time PCR) - (reply: 2)
20. Exact amplification in negative control??? - (reply: 1)
21. PCR is inconsistent - (reply: 4)
22. Specific primer problem - (reply: 2)
23. How to minimize delta ct value when everything else worked well in qRT PCR - (reply: 1)
24. How to minimize delta ct value when everything else worked well in qRT PCR - (reply: 1)
25. RAPD PCR - (reply: 2)
26. Instrument blocklower error - (reply: 2)
27. Long pcr frustration and problem.. all help is Much appreciated - (reply: 3)
28. calculating ramp rate on thermocycler - urgent - (reply: 1)
29. How to check if my primers were degraded? - (reply: 2)
30. problem with PCR amplification of cDNA - (reply: 4)