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1. How to design primer to amplify genomic DNA? - (reply: 2)
2. Overlap PCR, need help - (reply: 8)
3. Internal control for miRNA RT-PCR - (reply: 1)
4. Primers - (reply: 1)
5. Primers mix - (reply: 2)
6. degenerate bases in my sequences - (reply: 1)
7. Need help with dCAPS pcr, seeing huge bands on gel - (reply: 1)
8. How do I make GTE buffer for alkaline lysis? - (reply: 1)
9. Having problem with primers for qPCR - (reply: 4)
10. strange raw data - - (reply: 3)
11. need a method for oligonucleotide visualization - (reply: 5)
12. Bad fragment amplification - (reply: 4)
13. problem with ChiP q-PCR - (reply: 4)
14. suitable single-copy gene target for fungal qPCR - (reply: 4)
15. Increasing specificity of allele-specific Tq probes? - (reply: 2)
16. No amplification with TRAPEZE kit! - (reply: 1)
17. qPCR of Intronless gene - (reply: 4)
18. cDNA not enough-variability - (reply: 2)
19. Stargazer PCR problems - (reply: 3)
20. General PCR discussion - (reply: 8)
21. User error or pipette contamination - (reply: 3)
22. Reverse transcription 101 question! - (reply: 5)
23. Real time PCR doubt - (reply: 5)
24. High Ct values in ref gene as well as target gene - (reply: 3)
25. GADPH does not express - (reply: 1)
26. qPCR reference gene testing help - (reply: 1)
27. protocol to relieve melanin inhibition of PCR - (reply: 4)
28. No bands despite different primers and conditions and TAqs - (reply: 8)
29. Multiplex TaqMan-like Assay PCR Efficiency - (reply: 3)
30. Wrong PCR product - (reply: 2)
2. Overlap PCR, need help - (reply: 8)
3. Internal control for miRNA RT-PCR - (reply: 1)
4. Primers - (reply: 1)
5. Primers mix - (reply: 2)
6. degenerate bases in my sequences - (reply: 1)
7. Need help with dCAPS pcr, seeing huge bands on gel - (reply: 1)
8. How do I make GTE buffer for alkaline lysis? - (reply: 1)
9. Having problem with primers for qPCR - (reply: 4)
10. strange raw data - - (reply: 3)
11. need a method for oligonucleotide visualization - (reply: 5)
12. Bad fragment amplification - (reply: 4)
13. problem with ChiP q-PCR - (reply: 4)
14. suitable single-copy gene target for fungal qPCR - (reply: 4)
15. Increasing specificity of allele-specific Tq probes? - (reply: 2)
16. No amplification with TRAPEZE kit! - (reply: 1)
17. qPCR of Intronless gene - (reply: 4)
18. cDNA not enough-variability - (reply: 2)
19. Stargazer PCR problems - (reply: 3)
20. General PCR discussion - (reply: 8)
21. User error or pipette contamination - (reply: 3)
22. Reverse transcription 101 question! - (reply: 5)
23. Real time PCR doubt - (reply: 5)
24. High Ct values in ref gene as well as target gene - (reply: 3)
25. GADPH does not express - (reply: 1)
26. qPCR reference gene testing help - (reply: 1)
27. protocol to relieve melanin inhibition of PCR - (reply: 4)
28. No bands despite different primers and conditions and TAqs - (reply: 8)
29. Multiplex TaqMan-like Assay PCR Efficiency - (reply: 3)
30. Wrong PCR product - (reply: 2)