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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1. Denatured plasmid for pcr - (reply: 1)
2. Possible primer dimer problem..Need solution!!! - (reply: 2)
3. When to dilute samples for qPCR - (reply: 2)
4. Some info. on PCR products stability w/respect to shipping - (reply: 3)
5. 3' RACE troubles - (reply: 1)
6. haeting RNA before electrophoresis - (reply: 1)
7. PCR Products not run well in the GEL - (reply: 1)
8. Qubit Nanodrop Quantifications - (reply: 1)
9. Decontaminating DNA - Effective Means? - (reply: 4)
10. Consistancy issue with PCR block - (reply: 1)
11. Queries regarding PCR - (reply: 3)
12. Identification of Listeria monocytogenes - (reply: 1)
13. Calculation of Taq Polymerase - (reply: 3)
14. Best Taq for colony PCR? - (reply: 5)
15. Gene expression varying over time course when it shouldn't (qRT-PCR) - (reply: 6)
16. How to test my milleq water in q PCR about contaminants - (reply: 3)
17. different amount of cDNA in qRT-PCR for target and reference genes - (reply: 3)
18. real time PCR primer design - (reply: 1)
19. Plasmid Linearization Problems - (reply: 9)
20. Plasmid calculation question (Standard Curve Quantification) - (reply: 6)
21. Increasing RNA yield from very small tissues - (reply: 3)
22. Degenerated Primers and their problems - (reply: 1)
23. Interpreting primer BLAST scores for self-complementary - (reply: 5)
24. Testing primers on 'unknown' tissue - (reply: 1)
25. Second Hand Roche Lightcycler II maching - to buy or not to buy - (reply: 1)
26. nested PCR for low viral load- HBV patient sample - (reply: 2)
27. Finding an answer as to why I have Tm but no Ct - (reply: 6)
28. Amplification in HK gene but not for target gene - (reply: 3)
29. Missing DNA Target and Effect on Non-Specific Amplicons - (reply: 1)
30. 3 RACE-PCR of cDNA ends yielded a piece of 2 different genes? - (reply: 2)