magnetic beads - Magnetic beads (Jun/05/2009 )
Hello,
Does anyone have any idea if we could make our own magentic beads with poly T's attached to it? That is buy only the beads from a company and then coat it with the poly T's?
Would like to work and see if this is doable in the lab and also to see if I can try and use any other primer specific to an organism to capture the RNA.
thekid
-thekid-
thekid on Jun 5 2009, 11:29 AM said:
Hello,
Does anyone have any idea if we could make our own magentic beads with poly T's attached to it? That is buy only the beads from a company and then coat it with the poly T's?
Would like to work and see if this is doable in the lab and also to see if I can try and use any other primer specific to an organism to capture the RNA.
thekid
Does anyone have any idea if we could make our own magentic beads with poly T's attached to it? That is buy only the beads from a company and then coat it with the poly T's?
Would like to work and see if this is doable in the lab and also to see if I can try and use any other primer specific to an organism to capture the RNA.
thekid
You can get carboxylic beads (COOH) and order amine-oligos (either oligo-dT or specific primer) and conjugate both things using EDC reaction (the beads will come with protocol). I've used Invitrogen's MyOne before with specific primers to capture my target and worked really nice. Could PCR straight from them too.
-almost a doctor-
thekid on Jun 5 2009, 02:29 AM said:
Hello,
Does anyone have any idea if we could make our own magentic beads with poly T's attached to it? That is buy only the beads from a company and then coat it with the poly T's?
Would like to work and see if this is doable in the lab and also to see if I can try and use any other primer specific to an organism to capture the RNA.
thekid
Does anyone have any idea if we could make our own magentic beads with poly T's attached to it? That is buy only the beads from a company and then coat it with the poly T's?
Would like to work and see if this is doable in the lab and also to see if I can try and use any other primer specific to an organism to capture the RNA.
thekid
you can, I did something similar with non-magnetic beads.
the procedure, however, has it's tricky parts (mostly because EDC, a coupling reagent, is not stable).
this protocol might be of interest to you:
http://www.miraibio.com/view-document-deta...etic-beads.html
-warsel-