Can I redo a linearization - (Mar/31/2010 )

Hi All,

Got a bit of a problem. I was linearising a plasmid, cutting with BamH1 in one tube and Xba1 in the second (making sense and antisense probes) but I made a silly mistake and mixed up the buffers. Can I clean up the mix and isolate the plasmid again and redo???

Any help would be brilliant

Cheers

Yes, your DNA is still there. It might even be cut correctly. Most restriction enzymes will cut even if it is the wrong buffer.