chloroform extraction---become dark yellow - (May/06/2010 )
Today when I did phenol/chloroform extraction for the ChIP DNA, I found something strange. After adding chloroform to the aquar phase which extracted from phenol/chloroform/Isoamylalcohol, the organic phase turned to dark yellow...I found maybe due to chloroform is old, because the chloroform I used was a bit yellow...phenol/chlo/Isoamyl should be ok, it was a commercial one, the color is still light yellow...Is there anyone knows what is the dark yellow thing, will it break the DNA???
jenny0827 on May 6 2010, 10:06 PM said:
normally the phenol can turn yellow if old and oxidised...it's not recommended to use it then...
Oxidation of the phenol produces a pink/brown compound, and this compound will cause nicking of your DNA and degradation of your RNA.