Protocol Online logo
Top : New Forum Archives (2009-): : Molecular Biology

Positive control for Northern Blot - (Jul/14/2010 )

Hi everyone,

my boss ask me to have a good positive control for my northern blot.
I did a lot of lecture but I just saw negatif control.
So do you have a idea of what's a good controlo for a northern?

thanks

-Xavier-

Good positive control is something that you know contains the RNA sequence that you are looking for in your northerns. This could be from a cell line transfected with a plasmid.

-bob1-