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Any body has any idea on why DNA samples loaded on Native PAGE dont migrate all at the sam level?why this would happen? any suggestion?

-Fhannan-

different size, salt content of the sample, tilted gel (not level),...

show us a picture

-mdfenko-

No, they are all with the same size see the attached... Is it because the running buffer is not ok or APS is not fresh enough?
Attached Image

-Fhannan-

bubbles under the center of the gel (between the plates) could cause this.

-mdfenko-