dual luciferase data analysis - (Jun/27/2011 )
I am new to the Dual Luciferase system, so i needed some guidance. I am using Promega's dual luciferase kit.
So i did an assay transfecting cells with either
1. control TA-luc(ff)/renilla.luc or
For data analysis I divided the TEST(ff) numbers with its Renilla luc numbers.
What do i do with the control ? Do i divide control TA-luc(ff) numbers over its Renilla luc numbers, and plot it next to the test as separate bars..
do i divide the TEST ratios over the control TA-luc ratios and plot as a single bar.
Any help would be appreciated.
In general, I will always prefer a two bar graph. By doing so you are actually showing a control. If you normalize with the empty vector (TA-luc) it is like if you asking to the reader: 'believe me, I normalized'. If you show it, the reader will be more likely to believe the same data because his brain will normalize himself while seeing the graph.
In particular, I will not normalize twice, because the second normalization is going to delete the first one.
---------- x -------- = -------
thanks a lot.. this helps..