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Culture Related Discussions
  1. immunostaining of cells in culture - (reply: 1)
  2. Help!! need to culture PC3M cell line. Any advice? - (reply: 5)
  3. FIbroblast culture - (reply: 3)
  4. lymphocytes culture - (reply: 3)
  5. Days old culture, removal of CO2, pH rise or drop? - (reply: 1)
  6. Please help, may i replenish Glutamine in cell culture medium? - (reply: 3)
  7. cholesterol in milk cultures - (reply: 6)
  8. Elisa using HEK culture medium - (reply: 14)
  9. h9c2 cells culture - (reply: 3)
  10. Culture HEK293T with poly-lysine - (reply: 2)
  11. Human insulin for cell culture? - (reply: 2)
  12. changing cell culture media - (reply: 1)
  13. DMSO stock of overnight bacterial cultures - (reply: 1)
  14. latest research in plant tissue culture field - (reply: 1)
  15. Cell culture shared with yeast? - (reply: 2)
  16. rat primary hippocampal culture - (reply: 1)
  17. Antibiotic concentration in primary culture - (reply: 4)
  18. What kind of cell culture contamination is it? - (reply: 8)
  19. What's the function of cholera toxin in cell culture medium? eg. MCF10A cell - (reply: 2)
  20. Lymphoblastoid cell culture - (reply: 9)
  21. Determining gene of interest from Cell culture lines - (reply: 2)
  22. fatty acid free BSA-cell culture - (reply: 6)
  23. Foam in Cell Culture - (reply: 1)
  24. New cell culture user with no teacher or trainer - (reply: 5)
  25. Viral RNA Extraction from Tissue Culture Supernatant - (reply: 5)
  26. Potato tissue culture pH - (reply: 5)
  27. Cell culture in candle jar - (reply: 2)
  28. Contamination in human fibroblast culture - (reply: 9)
  29. Moving "rods" in human PBMC culture? - (reply: 4)
  30. Black moving particles in the cell Culture - (reply: 2)
  31. Technical question: Why use a bacterial starter culture? - (reply: 5)
  32. cell culture - (reply: 1)
  33. What antifungal to use in Tissue Culture??? - (reply: 2)
  34. HEK 293 cell culture - (reply: 3)
  35. Cell Culture debris or contamination? - (reply: 4)
  36. cell culture media refrigeration - (reply: 4)
  37. applying cell culture protocol of protein extraction for rat intact tissue is co - (reply: 1)
  38. Dropped e.coli (BL21) culture on clothes - (reply: 3)
  39. HepG2 culture conditions - (reply: 4)
  40. Blue Bacterial Culture without X-gal - (reply: 3)
  41. cell culture confusion - (reply: 1)
  42. stable transfection suspension cultures - (reply: 1)
  43. Adjusting pH of cell culture medium for ELISA - (reply: 1)
  44. To isolate DNA, RNA and Protein from same culture flask for methylation study? - (reply: 2)
  45. Tissue culture treatment of cover slip - Tissue culture treatment without using amine/ thiol containing buffers (reply: 5)
  46. Freezing culture media - (reply: 4)
  47. mouse bone marrow MSC culture - (reply: 3)
  48. Typical "dancing black dots" but living in spheres and with pics! - culture contamination (reply: 1)
  49. Cause of Death for Cell Culture? - (reply: 6)
  50. Help me brainstorm an explanation for this cell culture contamination - Cell Culture, contamination, NIH 3t3 (reply: 7)
  51. Mystery contamination - Tissue culture (reply: 4)
  52. Tissue culture hood vacuum (aspiration) tubing - What is the best way for storing it between uses? (reply: 1)
  53. Opening a sterile reagent out of the tissue culture hood - (reply: 4)
  54. Expired media - How long after the expiration data is cell culture media good? (reply: 2)
  55. bacterial Cell free culture supernatants - Can any one help me out to prepare a cell free culture supernatant (reply: 3)
  56. Oh my cell culture problems never cease!!! - (reply: 4)
  57. why 12 h culture for plasmid prep? - (reply: 2)
  58. MSC culture stops expanding? - (reply: 1)
  59. MSC in culture - stop growing? - Mesenchymal stem cells in culture randomly stop expanding (reply: 2)
  60. Is 2% pen strep too much for cell culture? - will my cells die a painful death? (reply: 3)
  61. SPONTAEOUS DEATH OF CELLS in culture - (reply: 8)
  62. help: why plasmid yield dropped ever since the first culture - (reply: 2)
  63. contamination issue in cell culture - (reply: 1)
  64. Yeast contamination in cell culture - (reply: 8)
  65. Rat Dental Pulp Stem Cell Culture - (reply: 1)
  66. Culture of mouse macrophages - Protocol (reply: 2)
  67. 1-day old culture for DNA extraction - plausible? - (reply: 2)
  68. weird & curious problem with CHO cell culture - (reply: 3)
  69. why do my hybridomas seem to stop producing antibodies when screened 2 weeks lat - hybridoma culture (reply: 5)
  70. cell culture media - glutamine! - (reply: 2)
  71. Neuronal Cell Culture Woes - Not even making it out of the gate! (reply: 6)
  72. MDA-MB-468 culture condition - (reply: 3)
  73. CTLL-2 culture - preventing cell clumping - (reply: 2)
  74. HepG2 cells culture conditions - (reply: 2)
  75. What is the proper way to infect cell culture with virus? - (reply: 5)
  76. Help Growth for Microglia cells - In culture for 18 days and only 2-3% confluent (reply: 2)
  77. Phage Contamination of Cultures? - Phage contamination or somethign else? (reply: 3)
  78. heat inactivation of serum - cell culture (reply: 3)
  79. Black dots in cell culture - (reply: 7)
  80. mold contamination in my bacterial culture - (reply: 16)
  81. CO2 level for cell culture - (reply: 6)
  82. THP-1 culture problem - (reply: 2)
  83. sphere and suspension culture subculturing - (reply: 1)
  84. adult cell culture - (reply: 1)
  85. Insulin supplements to cell culture media - (reply: 1)
  86. Bacteria to Cell Culture - How many bacteria should I add to cell culture (reply: 2)
  87. garlic tissue culture final project - (reply: 2)
  88. topic and cell culture - (reply: 2)
  89. min6 cells immunofourescence staining - staining and culture problem (reply: 4)
  90. Can I use frozen culture supernatant for ELISA - (reply: 1)
  91. Help! How to prepare 1 x 10^6 cell - cell culture (reply: 6)
  92. Neuronal Cell Culture - Media Changes - How to change media half and half (reply: 4)
  93. Liquid culture of N. meningitidis - advice needed! (reply: 1)
  94. Cell culture fasting - (reply: 1)
  95. Hybridoma culture problem. Help! - (reply: 2)
  96. Fungal Contamination? - Suspension culture (reply: 2)
  97. solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 1)
  98. A solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 6)
  99. Trying To Culture Oil Eating Microbes? - (reply: 1)
  100. Extremely Weird Issue - CHO Cell Culture (reply: 2)
  101. cell culture clarification - I need help please (reply: 2)
  102. Nuclear extraction on non-primary culture cells - Western blot (reply: 2)
  103. Looking for "biocell" from the forum archives. - Cell culture contamination problems.... (reply: 1)
  104. UV safety in cell culture hood - (reply: 3)
  105. Info about A7r5 cell culture - (reply: 1)
  106. Mixed Glial Culture on chamber slides - Cells wont grow in chamber slides (reply: 4)
  107. cell culture media - (reply: 3)
  108. DNA Extraction from bacterial cultures - (reply: 3)
  109. Growing RAW 264.7 in suspension culture - (reply: 9)
  110. what will happen if my cell culture have exposed to unfiltered air for a few sec - (reply: 2)
  111. Storage of Bacterial Culture at 4 degrees - (reply: 2)
  112. cell culture split ratios. - (reply: 2)
  113. MDA-MB-231 cell culture issue - after thawing first passage is fine, next goes bad (reply: 7)
  114. Attention-seeking cell cultures - reduce subculture ratio? - QM7 cells do the darndest things (reply: 2)
  115. Need pH 6 Cell culture media - (reply: 1)
  116. Cell culture contamination question - what the hell are these things?? - (reply: 3)
  117. 'Stringy' contamination of cells? - Weird stringy thing in cell culture flasks (reply: 3)
  118. Culture Media for stem cells - (reply: 1)
  119. bacterial culture - (reply: 4)
  120. 700-80% confluent culture - (reply: 1)
  121. E. coli not growing in liquid cultures :( - (reply: 4)
  122. (CELL CULTURE) Serious advice needed regarding my career..? - (reply: 2)
  123. Change of colour in cell culture media...? - (reply: 2)
  124. Toluidine blue staining for cell culture - (reply: 1)
  125. cell culture - 3D cell culture (reply: 1)
  126. Aspergillus in cell culture room - HELP!!! how hardcore to decontami - Cell culture, contamination (reply: 6)
  127. MEF culture contamination? - Contaminated cell culture? (reply: 2)
  128. rat primary cortical neurons - early death in culture (reply: 4)
  129. IP my protein from culture supernatant - (reply: 2)
  130. Tissue culture contamination - can't get rid of it!!! (reply: 6)
  131. what type of media for gingiva fibroblast tissue culture? - (reply: 3)
  132. Enzyme to Histology contrast in vessel culture - (reply: 4)
  133. cardiomyocytes primary culture - (reply: 3)
  134. how we count the passages in cell cultures? - (reply: 5)
  135. Primary neurones culture - Survival problem primary neurones culture (reply: 5)
  136. cell culture urgent!! - (reply: 2)
  137. Cell culture medium instead of Nucleofector solution?? - (reply: 3)
  138. Issue on mycoplasma detection and treatment in cell culture - (reply: 3)
  139. fibroblasts viability - cell culture method (reply: 1)
  140. Contamination in SH-SY5Y cultures, mycoplasma? - (reply: 2)
  141. how to stain suspension culture? - (reply: 1)
  142. E. Coli culture in microplate - (reply: 5)
  143. cell culture media - with or w/o L-glutamine? (reply: 2)
  144. Diluting rifampicin for culture use - (reply: 2)
  145. problems with adherent CHO culture - (reply: 4)
  146. Growing primary neuronal cultures on plastic? - Anyone used commercially available pre-treated plates? (reply: 2)
  147. Half life o acetaminophen - on culture media (reply: 1)
  148. Sodium Pyruvate - What is it's use in (NIH 3T3) cell culture? (reply: 4)
  149. overgrown culture - (reply: 7)
  150. Culture Needed: Burkholderia cepacia complex - Possible Collaboration or Culture Exchange (reply: 1)
  151. Adding drug to cultured cells - Cell culture, HEK293, drug (reply: 3)
  152. Whole Kidney primary cell culture - (reply: 1)
  153. What should be the goal amount of protein to get from purification? - Trying to figure out how much culture I need to make (reply: 3)
  154. What is DME medium for Hybridoma cell culture - is DME medium and DMEM medium are the same for hybridoma culture?? (reply: 1)
  155. Is there a problem by adding HCL to the cell culture medium in order to manipula - (reply: 1)
  156. Cell culture - (reply: 7)
  157. Dimethyl Sulfate (DMS) as carrier in cell cultures - (reply: 1)
  158. Very spall particulates vibrating in my culture - (reply: 2)
  159. SFFV promoter in DRG cultures - (reply: 2)
  160. Contamination in cell cultures - (reply: 9)
  161. Bacteria culture and growth curve? - (reply: 1)
  162. Macrophage dinamic in culture - (reply: 2)
  163. Opinion: Is my culture contaminated (yeasts)? - Pictures includede (reply: 2)
  164. Growing liquid cultures in microfuge tubes? - (reply: 3)
  165. Fibroblast cell culture - (reply: 1)
  166. Cell Culture procedure(manual, protocole, what ever ;) - (reply: 1)
  167. western blot using culture media - (reply: 4)
  168. Solutions for Collection and Culture of Dust Mites - appreciate any protienal ideas (reply: 5)
  169. What is this ? - Cell culture contaminant (reply: 2)
  170. How to culture cells after sorting? - (reply: 3)
  171. Cell culture media - require information on media (reply: 3)
  172. 'Black dots' in cell culture - (reply: 2)
  173. Starter culture dilution (preparation of competent cells) - I don't know how much should I dilute my starter culture... (reply: 3)
  174. Stem cells, primary culture - Literature - What are some good papers to read to get started in this area? (reply: 3)
  175. culture medium sterilization - (reply: 3)
  176. Modifying collagen stiffness / rigidity in 3d cell culture - (reply: 1)
  177. liquid nitrogen in cell culture - (reply: 2)
  178. differential cell counting in mixed cultures - (reply: 1)
  179. Culture Grow well but No Plasmid - (reply: 2)
  180. membrane type of cell culture filtration - (reply: 3)
  181. Sodium Hypochlorite to kill bacteria - Alternatives to autoclaving bacterial cultures (reply: 6)
  182. culture medium - remove something (reply: 1)
  183. Culturing cell in absence of any growth factors - Cell culture minus growth factors (reply: 2)
  184. Yeast contamination in cell culture - hood to blame? - (reply: 6)
  185. keratinocyte culture - (reply: 1)
  186. bicarbonate in cell culture: powder v. liquid? - (reply: 3)
  187. To break cell clumps - suspension culture related (reply: 4)
  188. hepes for culture out of co2 incubator? - have you done this before/know about it? (reply: 1)
  189. adding fungicidies to the media in plant tissue culture - (reply: 2)
  190. About tissue culture 96 well plates - (reply: 4)
  191. shRNA selection in suspension cell culture - (reply: 6)
  192. Ethanol/methanol/dmso for cell culture - (reply: 1)
  193. stored cytokines from supernatant cell culture - stored cytokines from supernatant cell culture (reply: 3)
  194. THP-1 culture protocol - (reply: 3)
  195. cell culture in 2 stack flask - (reply: 1)
  196. Hypoxia Treatment For Cell Culture - (reply: 4)
  197. Cell culture media - DMEM and DMEM/F-12 (reply: 1)
  198. Slow cell culture growth? - (reply: 3)
  199. cell aging - cell culture (reply: 2)
  200. HKG for neuronal cultures...? - (reply: 1)
  201. Cyanobacteria culture sterilization - (reply: 1)
  202. Do histology staningings work in cell culture? - (reply: 2)
  203. Stimulating/Drugging Mammilian Cells - What is the proper way to add drugs or stimulants to a cell culture? (reply: 3)
  204. solve MCF-7 culture trouble - (reply: 4)
  205. tissue culture and its problem??? - (reply: 6)
  206. HL-60 cultures - (reply: 1)
  207. Request Genomic DNA of Burkholderia mallei - possible: collaboration or exchange DNA with pseudomallei culture (reply: 8)
  208. DMSO and cellular culture - (reply: 6)
  209. Cell culture contamination - "outsider I.D." (reply: 2)
  210. SH-SYS5 cell culture for dummies... - (reply: 4)
  211. collagen staining in cell culture - (reply: 3)
  212. Hydrogen Peroxide (H2O2) Generator - Decontaminating tissue culture lab with H2O2. (reply: 1)
  213. antibiotic stability in culture medium - (reply: 1)
  214. Serum free culture media 293T cells? - (reply: 1)
  215. protein expression - Preparation of starter cultures (reply: 15)
  216. Culture conditions for cell treatments - (reply: 4)
  217. Macrophage cell culture - Strange particles persist in an otherwise healty looking MF culture (reply: 5)
  218. PFA Quenching in Staining of 2D and 3D MDCK Cultures - (reply: 1)
  219. Cell Culture and Pipette Tips - (reply: 4)
  220. Which cell line culture has endogenous Wnt1 expression? - (reply: 2)
  221. methylotrophic bacteria culture contamination - (reply: 1)
  222. CHO or VERO? - Cell culture (reply: 2)
  223. Oxygen diffusion through cell culture medium - (reply: 2)
  224. Culture of NIH3T3-CD40L - (reply: 5)
  225. help: bovine Intestinal Epithelial Cell Culture protocol - (reply: 4)
  226. MCMV culture and in vivo infection questions - (reply: 1)
  227. western blot problem: cell culture VS mouse tissue - (reply: 4)
  228. Mouse Renal fibroblast Primary Culture - (reply: 2)
  229. Overnight SURE cell culture in LB-carbenecillin - (reply: 4)
  230. Culture of soil bacteria - (reply: 11)
  231. how will i be able to culture tumor cells? - (reply: 3)
  232. Subculture of Vero cells - Tissue Culture (reply: 1)
  233. Glycerol stock - must we use fresh culture? - (reply: 6)
  234. strange dots in cell culture flask?? - U87 cell line culture has strange white dots (reply: 4)
  235. Plaques in my culture flask - (reply: 2)
  236. bacterial contamination in cell culture - (reply: 3)
  237. THP-1 cell culture - (reply: 1)
  238. is it useful to grow two fungal cultures together for production of enzyme? - (reply: 7)
  239. OD of bacteria at different culture volume - (reply: 6)
  240. Isolation of secreted proteins from culture media - (reply: 2)
  241. neuronal cultures detached from laminin- and fibronectin-coated plate but not po - (reply: 4)
  242. Fc Blocking in cell cultures - (reply: 1)
  243. Adherent cells versus suspension cells - how can i convert adherent cell culture to suspension culture (reply: 6)
  244. Qustion about MDA-MB-157 cells culture - (reply: 2)
  245. Folic acid solution for cell culture - (reply: 2)
  246. Methanol toxicity in cell culture - (reply: 15)
  247. Re-using Culture flasks - (reply: 19)
  248. Neuro2A and Neuroblastoma B104- how to culture? - (reply: 2)
  249. Neuro2A and Neuroblastoma B104- how to culture? - (reply: 3)
  250. DMSO usage in cell culture - (reply: 9)
  251. Cell culture container - (reply: 2)
  252. Cell culture conditions and antigen expression - (reply: 1)
  253. SiRNA- gene silencing in brain Organotypic slice cultures - (reply: 3)
  254. Dying Monocytes - Problem with cell culture of monocytes (reply: 1)
  255. Neuronal cell culture - why use neonatal brains? - (reply: 1)
  256. freezing of E.coli culture prior DNA prep - (reply: 5)
  257. Cell culture contamination (do not know what it was) - (reply: 1)
  258. culture C6/36 insect cell lines - (reply: 1)
  259. cell culture best practices - virus immortalized and non-immortalized cells (reply: 1)
  260. Culturing QT6 cell line - Ideal culture conditions (reply: 2)
  261. coating tissue culture lates with fibronectin - (reply: 1)
  262. wierd clot in LB culture - (reply: 5)
  263. Problem Maintaining pH in Culture Media - (reply: 4)
  264. Kits for estradiol, FSH, LH or androgens analysis in culture medium - has anybody used any (reply: 2)
  265. Intestinal cell culture: Protocol required - (reply: 1)
  266. Carbon di oxide free cell culture - (reply: 1)
  267. fibroblast primary culture - (reply: 8)
  268. What's this in my culture??? - Debris in liquid culture (reply: 3)
  269. Adding glycerol to nutrient broth culture? - (reply: 2)
  270. cell culture guideline from abcam - (reply: 5)
  271. Cancer cell culture exploded onto my face - (reply: 8)
  272. Limitations of Animal Tissue Cultures - (reply: 11)
  273. filter tips or regular tips for tissue culture? - (reply: 4)
  274. Adult Neurosphere Cultures - (reply: 4)
  275. Treating glassware for tissue culture - (reply: 3)
  276. SVZ culture preparation contamination - (reply: 1)
  277. rat versus mouse neuron culture - (reply: 2)
  278. Why the pH value decreased seriously in my algal culture? - (reply: 2)
  279. Why the pH value decreased seriously in algal culture? - (reply: 7)
  280. Culture media for Nitrifying Organisms - (reply: 1)
  281. Chikungunya Virus Culture - Chikungunya Virus culture in mammalian cells form serum sample of huma (reply: 2)
  282. EB formation - type of culture dish to use (reply: 2)
  283. Protein extraction from cell culture media - (reply: 9)
  284. Intestinal epithelial cell culture - (reply: 4)
  285. Effect of salt concentration on bacterial culture - (reply: 4)
  286. Chilling Bacterial Cultures Before Centrifugation - (reply: 3)
  287. Hepatocyte culture - (reply: 4)
  288. Buffers that are good for cell culture. - need buffering capacity around pH 5-7 (reply: 3)
  289. Culture of rat cortical neurons - E16 or E18? (Archived) - (reply: 2)
  290. E. coli colonies didn't grow up in liquid culture. -HELP- - (reply: 9)
  291. How to culture PC3 cell line - (reply: 3)