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Culture Related Discussions
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Debris in cell culture - (reply: 1)
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storage of culture plates - (reply: 3)
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Sample storage for ELISA (cell culture supernatant) - (reply: 2)
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Primary culture of fibroblast cells from rat/mouse - (reply: 4)
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Addition of antibiotic to the culture medium - (reply: 1)
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HS-5 cell culture "question" - (reply: 1)
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Community feedback needed to clarify terms for a Cell Culture Ontology - (reply: 1)
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How viable is cell culture for a model? - (reply: 2)
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Help with adherent cell culture - (reply: 7)
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Co- culture experiment - (reply: 6)
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Preparing supernatant from cell culture for western blot - (reply: 4)
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Not wearing gloves during tissue culture - (reply: 5)
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All Cultures in Refrigerator Appear to Die - (reply: 2)
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Identify cell culture contamination - (reply: 2)
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Adipose stem cell culture - (reply: 5)
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Pore formation in cells after adding amphotericin B in culture medium - (reply: 2)
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Can I pause a bacterial culture at 4°C - (reply: 2)
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Injection of human cell culture conditioned media into nude mice - (reply: 1)
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Problems with extraction of RNA from primary culture - (reply: 4)
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Quick question about making culture media. - (reply: 5)
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New to organotypic slice cultures: my slices seem to disintegrate during stainin - (reply: 1)
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BaF3 cell culture in 96 well plates - (reply: 2)
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Storing Bacterial Culture - (reply: 1)
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human lymphoblast culture - (reply: 1)
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Maintainence of confluent cultures - (reply: 1)
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Questions about overnight cultures - (reply: 7)
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different type of media for cell culture - (reply: 1)
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CLOUDINESS of RIN5F cell culture - (reply: 7)
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macrophage contamination in microglial culture - (reply: 2)
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Cancer cells culture - (reply: 1)
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How to achieve uniform cell distribution in culture vessle - (reply: 5)
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Cell culture contamination - (reply: 3)
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cell culture neural cells easier? - (reply: 6)
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Question - Corticosterone, cell culture - (reply: 1)
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Osteosarcoma cell culture problem - (reply: 7)
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Primary culture of breast tumor cells - (reply: 1)
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Taking Culture Medium Samples for LDH Assay - (reply: 2)
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PBMCs culture - (reply: 1)
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culture media for pancreatic cancer stem cells - (reply: 2)
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Difference between types of bacterial culture broths? - (reply: 4)
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Mycology expert ! Identification of fungus by plate culture morphology - (reply: 5)
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Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
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Which is a better way to culture cells after thawing ? - (reply: 2)
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Greenish tinge to bacterial culture - (reply: 3)
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insulin concentration for tissue culture - (reply: 3)
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What will happen if the cell culture medium is suddenly changed to another one? - (reply: 2)
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Eliminate erythrocytes from culture - (reply: 4)
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Grevillea Acanthifolia Tissue Culture Help - (reply: 1)
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Reagent used to adjust pH of cell culture media - (reply: 3)
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Powder indomethacin in cell culture media?? - (reply: 2)
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3D MAMMALIAN TISSUE culture - (reply: 1)
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small dots in cell culture - (reply: 2)
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Sf9 cell suspension culture - (reply: 5)
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Discrepancy between tissue and cell culture - (reply: 1)
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Determining the % of GFP/CHERRY cells in co-mixed culture - (reply: 3)
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Plant suspension culture - (reply: 1)
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serumm free culture of adherent cho cells - (reply: 1)
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Unknown contaminant in Ecoli culture - (reply: 4)
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N acetyl cysteine solution for cell culture - (reply: 3)
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Unknown floating substances in culture flask - (reply: 1)
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May have incorrectly thawed a bacterial culture from -80 C - (reply: 3)
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Unknown contaminant in S. cerevisiae culture - (reply: 1)
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Culture Supernatant Gives O.D even in the uncoated wells - (reply: 4)
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Establishing a primary tissue culture from surgically excised tumor - (reply: 1)
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SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
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contamination in yeast cell culture - (reply: 8)
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Tissue culture contamination like bacteria inside the cells. What is this kind o - (reply: 1)
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Antibiotics in culture - any difference in salt form vs pure? - (reply: 1)
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Culture media for BGMK-hDAF - (reply: 3)
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Undefinable particles in cell culture flask - (reply: 2)
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immunostaining of cells in culture - (reply: 1)
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Help!! need to culture PC3M cell line. Any advice? - (reply: 5)
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FIbroblast culture - (reply: 3)
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lymphocytes culture - (reply: 3)
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Days old culture, removal of CO2, pH rise or drop? - (reply: 1)
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Please help, may i replenish Glutamine in cell culture medium? - (reply: 3)
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cholesterol in milk cultures - (reply: 6)
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Elisa using HEK culture medium - (reply: 14)
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h9c2 cells culture - (reply: 5)
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Culture HEK293T with poly-lysine - (reply: 2)
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Human insulin for cell culture? - (reply: 2)
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changing cell culture media - (reply: 1)
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DMSO stock of overnight bacterial cultures - (reply: 1)
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latest research in plant tissue culture field - (reply: 1)
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Cell culture shared with yeast? - (reply: 2)
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rat primary hippocampal culture - (reply: 1)
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Antibiotic concentration in primary culture - (reply: 4)
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What kind of cell culture contamination is it? - (reply: 8)
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What's the function of cholera toxin in cell culture medium? eg. MCF10A cell - (reply: 2)
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Mouse splenocytes dying after 3 days in culture - (reply: 1)
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Lymphoblastoid cell culture - (reply: 9)
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Determining gene of interest from Cell culture lines - (reply: 2)
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fatty acid free BSA-cell culture - (reply: 6)
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Foam in Cell Culture - (reply: 1)
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New cell culture user with no teacher or trainer - (reply: 5)
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Viral RNA Extraction from Tissue Culture Supernatant - (reply: 5)
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Potato tissue culture pH - (reply: 5)
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Cell culture in candle jar - (reply: 2)
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Contamination in human fibroblast culture - (reply: 9)
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Moving "rods" in human PBMC culture? - (reply: 5)
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Black moving particles in the cell Culture - (reply: 2)
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Technical question: Why use a bacterial starter culture? - (reply: 5)
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cell culture - (reply: 1)
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What antifungal to use in Tissue Culture??? - (reply: 2)
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HEK 293 cell culture - (reply: 3)
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Cell Culture debris or contamination? - (reply: 4)
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cell culture media refrigeration - (reply: 4)
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applying cell culture protocol of protein extraction for rat intact tissue is co - (reply: 1)
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Dropped e.coli (BL21) culture on clothes - (reply: 3)
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HepG2 culture conditions - (reply: 4)
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Blue Bacterial Culture without X-gal - (reply: 3)
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cell culture confusion - (reply: 1)
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stable transfection suspension cultures - (reply: 1)
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Adjusting pH of cell culture medium for ELISA - (reply: 1)
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To isolate DNA, RNA and Protein from same culture flask for methylation study? - (reply: 2)
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Tissue culture treatment of cover slip - Tissue culture treatment without using amine/ thiol containing buffers (reply: 5)
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Freezing culture media - (reply: 4)
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mouse bone marrow MSC culture - (reply: 6)
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Typical "dancing black dots" but living in spheres and with pics! - culture contamination (reply: 1)
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Cause of Death for Cell Culture? - (reply: 5)
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Help me brainstorm an explanation for this cell culture contamination - Cell Culture, contamination, NIH 3t3 (reply: 7)
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Mystery contamination - Tissue culture (reply: 4)
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Tissue culture hood vacuum (aspiration) tubing - What is the best way for storing it between uses? (reply: 1)
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Opening a sterile reagent out of the tissue culture hood - (reply: 4)
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Expired media - How long after the expiration data is cell culture media good? (reply: 2)
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bacterial Cell free culture supernatants - Can any one help me out to prepare a cell free culture supernatant (reply: 3)
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Oh my cell culture problems never cease!!! - (reply: 4)
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why 12 h culture for plasmid prep? - (reply: 2)
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MSC culture stops expanding? - (reply: 1)
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MSC in culture - stop growing? - Mesenchymal stem cells in culture randomly stop expanding (reply: 2)
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Is 2% pen strep too much for cell culture? - will my cells die a painful death? (reply: 3)
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SPONTAEOUS DEATH OF CELLS in culture - (reply: 8)
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help: why plasmid yield dropped ever since the first culture - (reply: 2)
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contamination issue in cell culture - (reply: 1)
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Yeast contamination in cell culture - (reply: 8)
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Rat Dental Pulp Stem Cell Culture - (reply: 1)
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Culture of mouse macrophages - Protocol (reply: 2)
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1-day old culture for DNA extraction - plausible? - (reply: 2)
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weird & curious problem with CHO cell culture - (reply: 3)
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why do my hybridomas seem to stop producing antibodies when screened 2 weeks lat - hybridoma culture (reply: 5)
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cell culture media - glutamine! - (reply: 2)
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Neuronal Cell Culture Woes - Not even making it out of the gate! (reply: 7)
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MDA-MB-468 culture condition - (reply: 3)
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CTLL-2 culture - preventing cell clumping - (reply: 2)
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HepG2 cells culture conditions - (reply: 2)
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What is the proper way to infect cell culture with virus? - (reply: 5)
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Help Growth for Microglia cells - In culture for 18 days and only 2-3% confluent (reply: 2)
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Phage Contamination of Cultures? - Phage contamination or somethign else? (reply: 3)
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heat inactivation of serum - cell culture (reply: 3)
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Black dots in cell culture - (reply: 7)
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mold contamination in my bacterial culture - (reply: 16)
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CO2 level for cell culture - (reply: 6)
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THP-1 culture problem - (reply: 2)
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sphere and suspension culture subculturing - (reply: 1)
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adult cell culture - (reply: 1)
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Insulin supplements to cell culture media - (reply: 1)
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Bacteria to Cell Culture - How many bacteria should I add to cell culture (reply: 2)
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garlic tissue culture final project - (reply: 2)
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topic and cell culture - (reply: 2)
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min6 cells immunofourescence staining - staining and culture problem (reply: 4)
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Can I use frozen culture supernatant for ELISA - (reply: 1)
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Help! How to prepare 1 x 10^6 cell - cell culture (reply: 6)
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Neuronal Cell Culture - Media Changes - How to change media half and half (reply: 4)
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Liquid culture of N. meningitidis - advice needed! (reply: 1)
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Cell culture fasting - (reply: 1)
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Hybridoma culture problem. Help! - (reply: 2)
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Fungal Contamination? - Suspension culture (reply: 2)
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solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 1)
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A solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 6)
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Trying To Culture Oil Eating Microbes? - (reply: 1)
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Extremely Weird Issue - CHO Cell Culture (reply: 2)
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cell culture clarification - I need help please (reply: 2)
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Nuclear extraction on non-primary culture cells - Western blot (reply: 2)
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Looking for "biocell" from the forum archives. - Cell culture contamination problems.... (reply: 1)
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UV safety in cell culture hood - (reply: 3)
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Info about A7r5 cell culture - (reply: 1)
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Mixed Glial Culture on chamber slides - Cells wont grow in chamber slides (reply: 4)
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cell culture media - (reply: 3)
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DNA Extraction from bacterial cultures - (reply: 3)
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Growing RAW 264.7 in suspension culture - (reply: 9)
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what will happen if my cell culture have exposed to unfiltered air for a few sec - (reply: 2)
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Storage of Bacterial Culture at 4 degrees - (reply: 2)
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cell culture split ratios. - (reply: 2)
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MDA-MB-231 cell culture issue - after thawing first passage is fine, next goes bad (reply: 7)
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Attention-seeking cell cultures - reduce subculture ratio? - QM7 cells do the darndest things (reply: 2)
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Need pH 6 Cell culture media - (reply: 1)
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Cell culture contamination question - what the hell are these things?? - (reply: 3)
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'Stringy' contamination of cells? - Weird stringy thing in cell culture flasks (reply: 3)
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Culture Media for stem cells - (reply: 1)
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bacterial culture - (reply: 4)
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700-80% confluent culture - (reply: 1)
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E. coli not growing in liquid cultures :( - (reply: 4)
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(CELL CULTURE) Serious advice needed regarding my career..? - (reply: 2)
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Change of colour in cell culture media...? - (reply: 2)
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Toluidine blue staining for cell culture - (reply: 1)
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cell culture - 3D cell culture (reply: 1)
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Aspergillus in cell culture room - HELP!!! how hardcore to decontami - Cell culture, contamination (reply: 6)
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MEF culture contamination? - Contaminated cell culture? (reply: 2)
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rat primary cortical neurons - early death in culture (reply: 4)
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IP my protein from culture supernatant - (reply: 2)
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Tissue culture contamination - can't get rid of it!!! (reply: 6)
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what type of media for gingiva fibroblast tissue culture? - (reply: 3)
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Enzyme to Histology contrast in vessel culture - (reply: 4)
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cardiomyocytes primary culture - (reply: 3)
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how we count the passages in cell cultures? - (reply: 5)
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Primary neurones culture - Survival problem primary neurones culture (reply: 5)
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cell culture urgent!! - (reply: 2)
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Cell culture medium instead of Nucleofector solution?? - (reply: 3)
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Issue on mycoplasma detection and treatment in cell culture - (reply: 3)
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fibroblasts viability - cell culture method (reply: 1)
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Contamination in SH-SY5Y cultures, mycoplasma? - (reply: 2)
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how to stain suspension culture? - (reply: 1)
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E. Coli culture in microplate - (reply: 5)
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cell culture media - with or w/o L-glutamine? (reply: 2)
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Diluting rifampicin for culture use - (reply: 2)
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problems with adherent CHO culture - (reply: 4)
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Growing primary neuronal cultures on plastic? - Anyone used commercially available pre-treated plates? (reply: 2)
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Half life o acetaminophen - on culture media (reply: 1)
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Sodium Pyruvate - What is it's use in (NIH 3T3) cell culture? (reply: 4)
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overgrown culture - (reply: 7)
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Culture Needed: Burkholderia cepacia complex - Possible Collaboration or Culture Exchange (reply: 1)
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Adding drug to cultured cells - Cell culture, HEK293, drug (reply: 3)
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Whole Kidney primary cell culture - (reply: 1)
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What should be the goal amount of protein to get from purification? - Trying to figure out how much culture I need to make (reply: 3)
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What is DME medium for Hybridoma cell culture - is DME medium and DMEM medium are the same for hybridoma culture?? (reply: 1)
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Is there a problem by adding HCL to the cell culture medium in order to manipula - (reply: 1)
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Cell culture - (reply: 7)
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Dimethyl Sulfate (DMS) as carrier in cell cultures - (reply: 1)
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Very spall particulates vibrating in my culture - (reply: 2)
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SFFV promoter in DRG cultures - (reply: 2)
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Contamination in cell cultures - (reply: 9)
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Bacteria culture and growth curve? - (reply: 1)
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Macrophage dinamic in culture - (reply: 2)
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Opinion: Is my culture contaminated (yeasts)? - Pictures includede (reply: 2)
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Growing liquid cultures in microfuge tubes? - (reply: 3)
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Fibroblast cell culture - (reply: 1)
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Cell Culture procedure(manual, protocole, what ever ;) - (reply: 1)
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western blot using culture media - (reply: 4)
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Solutions for Collection and Culture of Dust Mites - appreciate any protienal ideas (reply: 5)
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What is this ? - Cell culture contaminant (reply: 2)
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How to culture cells after sorting? - (reply: 3)
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Cell culture media - require information on media (reply: 3)
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'Black dots' in cell culture - (reply: 2)
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Starter culture dilution (preparation of competent cells) - I don't know how much should I dilute my starter culture... (reply: 3)
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Stem cells, primary culture - Literature - What are some good papers to read to get started in this area? (reply: 3)
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culture medium sterilization - (reply: 3)
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Modifying collagen stiffness / rigidity in 3d cell culture - (reply: 1)
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liquid nitrogen in cell culture - (reply: 2)
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differential cell counting in mixed cultures - (reply: 1)
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Culture Grow well but No Plasmid - (reply: 2)
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membrane type of cell culture filtration - (reply: 3)
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Sodium Hypochlorite to kill bacteria - Alternatives to autoclaving bacterial cultures (reply: 6)
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culture medium - remove something (reply: 1)
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Culturing cell in absence of any growth factors - Cell culture minus growth factors (reply: 1)
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Yeast contamination in cell culture - hood to blame? - (reply: 6)
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keratinocyte culture - (reply: 1)
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bicarbonate in cell culture: powder v. liquid? - (reply: 3)
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To break cell clumps - suspension culture related (reply: 4)
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hepes for culture out of co2 incubator? - have you done this before/know about it? (reply: 1)
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adding fungicidies to the media in plant tissue culture - (reply: 2)
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About tissue culture 96 well plates - (reply: 4)
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shRNA selection in suspension cell culture - (reply: 6)
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Ethanol/methanol/dmso for cell culture - (reply: 1)
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stored cytokines from supernatant cell culture - stored cytokines from supernatant cell culture (reply: 3)
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THP-1 culture protocol - (reply: 4)
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cell culture in 2 stack flask - (reply: 1)
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Hypoxia Treatment For Cell Culture - (reply: 4)
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Cell culture media - DMEM and DMEM/F-12 (reply: 1)
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Slow cell culture growth? - (reply: 3)
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cell aging - cell culture (reply: 2)
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HKG for neuronal cultures...? - (reply: 1)
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Cyanobacteria culture sterilization - (reply: 1)
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Do histology staningings work in cell culture? - (reply: 2)
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Stimulating/Drugging Mammilian Cells - What is the proper way to add drugs or stimulants to a cell culture? (reply: 3)
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solve MCF-7 culture trouble - (reply: 4)
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tissue culture and its problem??? - (reply: 6)
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HL-60 cultures - (reply: 1)
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Request Genomic DNA of Burkholderia mallei - possible: collaboration or exchange DNA with pseudomallei culture (reply: 8)
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DMSO and cellular culture - (reply: 6)
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presentation about plant tissue culture - (reply: 1)
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Cell culture contamination - "outsider I.D." (reply: 2)
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SH-SYS5 cell culture for dummies... - (reply: 4)
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collagen staining in cell culture - (reply: 5)
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Hydrogen Peroxide (H2O2) Generator - Decontaminating tissue culture lab with H2O2. (reply: 1)
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antibiotic stability in culture medium - (reply: 1)
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Serum free culture media 293T cells? - (reply: 1)
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protein expression - Preparation of starter cultures (reply: 15)
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Culture conditions for cell treatments - (reply: 4)
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Macrophage cell culture - Strange particles persist in an otherwise healty looking MF culture (reply: 5)
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PFA Quenching in Staining of 2D and 3D MDCK Cultures - (reply: 1)
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Cell Culture and Pipette Tips - (reply: 4)
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Which cell line culture has endogenous Wnt1 expression? - (reply: 2)
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methylotrophic bacteria culture contamination - (reply: 1)
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CHO or VERO? - Cell culture (reply: 2)
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Oxygen diffusion through cell culture medium - (reply: 2)
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Culture of NIH3T3-CD40L - (reply: 5)
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help: bovine Intestinal Epithelial Cell Culture protocol - (reply: 4)
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MCMV culture and in vivo infection questions - (reply: 1)
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western blot problem: cell culture VS mouse tissue - (reply: 4)
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Mouse Renal fibroblast Primary Culture - (reply: 2)
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Overnight SURE cell culture in LB-carbenecillin - (reply: 4)
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Culture of soil bacteria - (reply: 11)
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how will i be able to culture tumor cells? - (reply: 3)
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Subculture of Vero cells - Tissue Culture (reply: 1)
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Glycerol stock - must we use fresh culture? - (reply: 6)
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strange dots in cell culture flask?? - U87 cell line culture has strange white dots (reply: 4)
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Plaques in my culture flask - (reply: 2)
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bacterial contamination in cell culture - (reply: 3)
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THP-1 cell culture - (reply: 1)
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is it useful to grow two fungal cultures together for production of enzyme? - (reply: 7)
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OD of bacteria at different culture volume - (reply: 6)
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Isolation of secreted proteins from culture media - (reply: 2)
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neuronal cultures detached from laminin- and fibronectin-coated plate but not po - (reply: 5)
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Adherent cells versus suspension cells - how can i convert adherent cell culture to suspension culture (reply: 6)
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Qustion about MDA-MB-157 cells culture - (reply: 2)
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Folic acid solution for cell culture - (reply: 2)
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Methanol toxicity in cell culture - (reply: 15)
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Re-using Culture flasks - (reply: 24)
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Neuro2A and Neuroblastoma B104- how to culture? - (reply: 2)
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Neuro2A and Neuroblastoma B104- how to culture? - (reply: 3)
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DMSO usage in cell culture - (reply: 9)
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Cell culture container - (reply: 2)
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Cell culture conditions and antigen expression - (reply: 1)
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SiRNA- gene silencing in brain Organotypic slice cultures - (reply: 3)
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Neuronal cell culture - why use neonatal brains? - (reply: 1)
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freezing of E.coli culture prior DNA prep - (reply: 5)
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Cell culture contamination (do not know what it was) - (reply: 1)
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culture C6/36 insect cell lines - (reply: 1)
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cell culture best practices - virus immortalized and non-immortalized cells (reply: 1)
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Culturing QT6 cell line - Ideal culture conditions (reply: 2)
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coating tissue culture lates with fibronectin - (reply: 1)
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wierd clot in LB culture - (reply: 5)
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Problem Maintaining pH in Culture Media - (reply: 4)
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Kits for estradiol, FSH, LH or androgens analysis in culture medium - has anybody used any (reply: 2)
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Intestinal cell culture: Protocol required - (reply: 1)
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Carbon di oxide free cell culture - (reply: 1)
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fibroblast primary culture - (reply: 8)
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What's this in my culture??? - Debris in liquid culture (reply: 3)
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Adding glycerol to nutrient broth culture? - (reply: 2)
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cell culture guideline from abcam - (reply: 5)
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Cancer cell culture exploded onto my face - (reply: 8)
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Limitations of Animal Tissue Cultures - (reply: 11)
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filter tips or regular tips for tissue culture? - (reply: 4)
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Adult Neurosphere Cultures - (reply: 4)
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Treating glassware for tissue culture - (reply: 3)
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SVZ culture preparation contamination - (reply: 1)
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rat versus mouse neuron culture - (reply: 2)
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Why the pH value decreased seriously in my algal culture? - (reply: 2)
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Why the pH value decreased seriously in algal culture? - (reply: 7)
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Culture media for Nitrifying Organisms - (reply: 1)
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Chikungunya Virus Culture - Chikungunya Virus culture in mammalian cells form serum sample of huma (reply: 2)
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EB formation - type of culture dish to use (reply: 2)
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Protein extraction from cell culture media - (reply: 9)
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Intestinal epithelial cell culture - (reply: 4)
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Effect of salt concentration on bacterial culture - (reply: 4)
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Chilling Bacterial Cultures Before Centrifugation - (reply: 3)
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Hepatocyte culture - (reply: 4)
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Buffers that are good for cell culture. - need buffering capacity around pH 5-7 (reply: 3)
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Culture of rat cortical neurons - E16 or E18? (Archived) - (reply: 2)
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E. coli colonies didn't grow up in liquid culture. -HELP- - (reply: 9)
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How to culture PC3 cell line - (reply: 3)