mold contamination in my bacterial culture - (Jan/25/2011 )
hello everybody!
I have a problem, my cell cultures have mold contamination since one month, checked the media, its ok and i changed the laminar hood, do cleaning as much as, still couldnt get rid of them 
Does anybody have any suggestion for killing mold,
please helping
thank you
P.S. my cultures are C. perfringens...
So the media is ok?
Then either your bacterial sample is contaminated or the flow/working proces is not good.
You have a bacterial sample in a tube or?
And the medium, you do make fresh medium?
Do others work in your lab with molds?
Spores are everywhere..
Hola, in cell cultures we use amphotericyn to avoid mold contaminations unless sometimes they appears. I donīt now clostridium sensitivity, but why donīt you try seed your cultute in a plate in isolation and take a new clean clostridium colony?Buena suerte
I would not use AmphoB in this case, you do not know anything about the contaminant and too many fungi have a resistance against it.
Is your culture of C perfingens really kept anaerob? Because if it is you should take a closer look at the mould...not many moulds can grow really anaerobically. You should check.
You could try heating your culture to 80/90 °C for some minutes (e.g. 10 min), C perfingens should be able to survive this, but most fungi will be killed. You can also make a time/temperature experiment (temperature resistance test), plate it and at some point the mould should disappear.
pito on Tue Jan 25 22:04:55 2011 said:
So the media is ok?
Then either your bacterial sample is contaminated or the flow/working proces is not good.
You have a bacterial sample in a tube or?
And the medium, you do make fresh medium?
Do others work in your lab with molds?
Spores are everywhere..
thank you,
I try all of them but unfortunatly:( the result doent change it:(
there are not only molds in tube but also media:(
gebirgsziege on Wed Jan 26 08:06:53 2011 said:
I would not use AmphoB in this case, you do not know anything about the contaminant and too many fungi have a resistance against it.
Is your culture of C perfingens really kept anaerob? Because if it is you should take a closer look at the mould...not many moulds can grow really anaerobically. You should check.
You could try heating your culture to 80/90 °C for some minutes (e.g. 10 min), C perfingens should be able to survive this, but most fungi will be killed. You can also make a time/temperature experiment (temperature resistance test), plate it and at some point the mould should disappear.
thanks gebirgsziege!
I will try heating the culture as a result the vegetative. C. perfringens turn to spore form that can be problem for me because I need veg. perfringens...
rickyvets on Wed Jan 26 21:39:18 2011 said:
pito on Tue Jan 25 22:04:55 2011 said:
So the media is ok?
Then either your bacterial sample is contaminated or the flow/working proces is not good.
You have a bacterial sample in a tube or?
And the medium, you do make fresh medium?
Do others work in your lab with molds?
Spores are everywhere..
thank you,
I try all of them but unfortunatly:( the result doent change it:(
there are not only molds in tube but also media:(
you have molds in your media? Even when you make fresh media ?
The heating might be a problem for you, but as allready been said here: yours grow without oxigen.. the molds need (most of them) oxigen...
pito on Wed Jan 26 22:06:23 2011 said:
rickyvets on Wed Jan 26 21:39:18 2011 said:
pito on Tue Jan 25 22:04:55 2011 said:
So the media is ok?
Then either your bacterial sample is contaminated or the flow/working proces is not good.
You have a bacterial sample in a tube or?
And the medium, you do make fresh medium?
Do others work in your lab with molds?
Spores are everywhere..
thank you,
I try all of them but unfortunatly:( the result doent change it:(
there are not only molds in tube but also media:(
you have molds in your media? Even when you make fresh media ?
The heating might be a problem for you, but as allready been said here: yours grow without oxigen.. the molds need (most of them) oxigen...
of course you r right how it can grow without oxigen but ı dont understand... ı look the media and the tubes still there are molds a short while ago:( I have used anaerocult and anaerobic jar for anaerobic condition on the other hand parafilm and liqued paraffin for tubes...
rickyvets on Wed Jan 26 21:47:46 2011 said:
gebirgsziege on Wed Jan 26 08:06:53 2011 said:
I would not use AmphoB in this case, you do not know anything about the contaminant and too many fungi have a resistance against it.
Is your culture of C perfingens really kept anaerob? Because if it is you should take a closer look at the mould...not many moulds can grow really anaerobically. You should check.
You could try heating your culture to 80/90 °C for some minutes (e.g. 10 min), C perfingens should be able to survive this, but most fungi will be killed. You can also make a time/temperature experiment (temperature resistance test), plate it and at some point the mould should disappear.
thanks gebirgsziege!
I will try heating the culture as a result the vegetative. C. perfringens turn to spore form that can be problem for me because I need veg. perfringens...
But when you subculture you pure bacteria more than twice the spores should be away.
If even the fresh medium without inoculation is contaminated, you have either a problem with your aseptic technique or your autoclave. You should check this!
gebirgsziege on Thu Jan 27 08:10:29 2011 said:
rickyvets on Wed Jan 26 21:47:46 2011 said:
gebirgsziege on Wed Jan 26 08:06:53 2011 said:
I would not use AmphoB in this case, you do not know anything about the contaminant and too many fungi have a resistance against it.
Is your culture of C perfingens really kept anaerob? Because if it is you should take a closer look at the mould...not many moulds can grow really anaerobically. You should check.
You could try heating your culture to 80/90 °C for some minutes (e.g. 10 min), C perfingens should be able to survive this, but most fungi will be killed. You can also make a time/temperature experiment (temperature resistance test), plate it and at some point the mould should disappear.
thanks gebirgsziege!
I will try heating the culture as a result the vegetative. C. perfringens turn to spore form that can be problem for me because I need veg. perfringens...
But when you subculture you pure bacteria more than twice the spores should be away.
If even the fresh medium without inoculation is contaminated, you have either a problem with your aseptic technique or your autoclave. You should check this!
I suppose there is no point in subcultering them if the medium itself is contaminated.
But as you allready said: there is something wrong..
Anyway: did you store the medium (do not add anything) anaerobically? Just try this for once (if you still have some left over without contamination) and see if you still get growth in your medium (or is all your medium allready been contaminated?)
(its possible the contamination happens during the storage in an aerobic storage room.. on the other hand: it should really happen... but you never know I suppose).