Can I use frozen culture supernatant for ELISA - (Jan/07/2011 )
I am trying to measure IgG secretion of cells in culture. I need to take samples every 2 days during an 8-day period. I am wondering if I could collect the samples and freez them untill the end of the culture and then measure them all at once? Thank you!
Hola, Yes you can, at the time to do an ELISA, you collect all the store samples, and commercial controls to follow a purification or a cellular production. The problem would be that the samples would be degradated, but freezing isnīt the case excep in some repeated freezing and tawing process. Buena suerte