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Opening a sterile reagent out of the tissue culture hood - (Jun/19/2011 )

I accidentally opened a sterile container of DMSO out of the tissue culture hood. It was sterile, so it was not supposed to be opened outside. I did not open it all the way and immediately closed the bottle back. What are the chances that it is contaminated?

-distalless-

The chances are actually fairly low... however do you want to take that risk???

-bob1-

If you really want to use it: fill a tube with LB and a tube with YP, add your dmso and incubate at the appropiate temperatures, check after a few days for growth

-fysio lab-

or, just sterile filter it....

-briguy7-

Note - many filter types will dissolve in DMSO, choose a membrane type that doesn't!

-bob1-