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heat inactivation of serum - cell culture (Feb/07/2011 )

Hello,

I am working with blood cells, and in this case it is important to inactivate the serum to be used in the culture. My colleague did that at 37C. Does this ensure the inactivation? I see that many people do it at 56C for 30 mins.

Thanks in advance

Hussein

-Hussein El Saghire-

37 is not enough - otherwise the serum would be inactivated in a normal animal (cows also function at about 37). 56 deg C for 45 minutes is how I have always done it.

-bob1-

bob1 on Tue Feb 8 22:23:21 2011 said:


37 is not enough - otherwise the serum would be inactivated in a normal animal (cows also function at about 37). 56 deg C for 45 minutes is how I have always done it.


Can u tell me pls why is there a need to inactivate serum ? Sorry for a very basic question.

-jasmine0507-

It depends on what you are working with, but the serum contains a bunch of stuff from the blood that can cause activation of things like the complement cascade if you are working with immunogen responsive cells such as lymphocytes.

-bob1-