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fibroblasts viability - cell culture method (Jun/03/2010 )

Hi,
i'm a student and a newbie in the field of cell culture and the problem relates to my thesis project. I would like to test the cell viability of fibrioblasts on a rough surface. Previously light microscopy images has been used and viability has been determined based on the cell's morphology, which for me seems to be a time consuming method and not that reliable! First alternative I thought of was to stain the cell and based on that count the cells, so my first question:

Is there any live and dead stains that can be combined that can be observed in a light microspopy?

This will probably solve the reliable problem, but not the time consuming.. so I thought of an MTT-assay, which will quantify the viable cells. If I am correct? I have two questions here:

Will this method work with fibroblast?

How precise is this method? it is probable that the results of the surfaces tested do not differ that much.

Best regards
Jess.

-jessica86-

Hi there,
MTT and MTS are quite stardard viability test. it should be ok to be used.
However, u did mention about rough surface.. wat u mean by that?

BTW, are u malaysian?

-tongck-