denaturation step of melting surve analysis - (Jul/16/2009 )

Hi all~ I am new in QPCR, and i have a basic question concerning SYBR melting curve.

I am wondering why is there a default 95C denaturation prior to the drop to 60C
(i.e. --> 95C x 15sec --> 60C x 1 min --> gradual increase to 95C)
what is the purpose of this step and what if i dont do that?

Many thanks !!

chroococcidiopsis on Jul 16 2009, 07:33 PM said:

All unique PCR products have unique nucleotide composition, and depending upon that, a unique melting curve. This step is done to see that your reaction amplifies only the product of your interest (single peak), rather than some non-specific products too (more than 1 peaks).

Thanks cellcounter
But what if i dont do the 95C denaturation? that is, after PCR, i go straight to 60C and gradual increase to 95C?

many thanks !! (& sorry if this is a dumb Q..)