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Real-time efficiency question - (Jun/05/2013 )

cDNA was left out on the bench top over the weekend. qPCR was run anyway to see if the samples could be salvaged, but the efficiency numbers were in the 200-250% range. The thought is that the cDNA degraded and so the primers are sticking to pieces of DNA in excess of what would be expected. Is this correct?


Run it on a gel and see what kind of banding patterns you have. Your hypothesis is a possibility. Just depends on what the curves look like.