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any one tried in-vitro expression system ? - Roche is the best ? (Dec/14/2009 )

I want to use a in-vitro expression system for fast and high-thoughput protein expression.....

but these kit are quite expensive.....

do you know which one is the best ?

Thx :lol:


I'm using Ambion mMessage machine for RNA transcription and promega rabbit reticulocyte lysate system for in vitro translation. I've used it three times and got mixed results. I don't know if the problem lies with transcription or translation or my technique. Anyway my bit of opinion though.