How long is "overnight"? - (Sep/18/2010 )
Often we need to prepare overnight culture. But how many hours is considered overnight?
Sometimes i prepare my o/n culture in the morning and sometimes evening...
-hianghao-
Cultures grow pretty good in abt 8hrs
overnight is usually from 5.00pm to next day 8.am
-ranvi-
To me, it's starting the culture at the end of the work day and growing it until the beginning of the next work day. Usually if I'm writing a protocol, however, I'll say "grow overnight for 16 hrs" or "grow overnight 24 hrs" or "grow overnight 12-16 hours". Something along those lines so that it's not too cryptic.
-fishdoc-
16 hours or thereabouts, I would say 16-18 hours.
-bob1-