PI needs something ASAP! - densitometry on westerns (Feb/10/2011 )
I have a bunch of western blot results of mitochondrial complexes which i obtained using 2D gels followed by western
PI wants me to do densitometry on them for quantitation
Now issue is - i don't have anything to compare it with. I have a single blot of complex I ... and even the molecular ladder does not show up
i can get intensity values for my band but how does that ever help me? i can't compare it with anything. is there a way i can know the quantity of my protein expressed
you can use a biotinylated ladder and process with a biotin-avidin hrp or ap (depending on which enzyme you use for your western blots).