restriction digestion - (Feb/16/2011 )
Hi all
Can the restricted plasmid with overhangs stay stable when stored in -20C before using for cloning purpose? Do the overhangs get damaged by thawing once?
-deespike-
constant freeze thawing can degrade the DNA but it should be ok once.
cells should be viable for up to a year at -70oC though
-jc545staffy-