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restriction digestion - (Feb/16/2011 )

Hi all

Can the restricted plasmid with overhangs stay stable when stored in -20C before using for cloning purpose? Do the overhangs get damaged by thawing once?

-deespike-

constant freeze thawing can degrade the DNA but it should be ok once.
cells should be viable for up to a year at -70oC though

-jc545staffy-