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a robust multi-gene cloning method - (Feb/02/2012 )

Here is a robust multi-gene cloning method developped in our lab. it is easy to use and require nothing special. The efficiency is much higher than that of restriction-ligation based methods.
please return your comments.


This is very nice work. I would be interested to know your approach to repetitive sequence assembly, such as a construct with two closely related variants of GFP, for example.


Really interesting. I would like to see how they behave with viral vectors construction where there are inverted repeats like in AAV or lentivirus plasmids.