a robust multi-gene cloning method - (Feb/01/2012 )
Here is a robust multi-gene cloning method developped in our lab. it is easy to use and require nothing special. The efficiency is much higher than that of restriction-ligation based methods. http://dx.plos.org/10.1371/journal.pone.0030267
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This is very nice work. I would be interested to know your approach to repetitive sequence assembly, such as a construct with two closely related variants of GFP, for example.
Really interesting. I would like to see how they behave with viral vectors construction where there are inverted repeats like in AAV or lentivirus plasmids.