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Methylation Related Discussions
  1. About DNMT1 antibody - (reply: 1)
  2. strang phenonmenon in MSP experiment - (reply: 2)
  3. restriction enzymes that cut at methylated cytosines - (reply: 2)
  4. methylnick.please help me with MSP primer designing! - (reply: 3)
  5. No Product from MethylEasy Kit Bisulfite Treatment! - (reply: 3)
  6. About DAPK1 MSP primer design. - About MSP primer design. (reply: 4)
  7. Questions about primer design for BSP in plant - shouldn't we take the mC in CHG CHH context into consideration? (reply: 3)
  8. methylation specific PCR - (reply: 5)
  9. Please help me design MSP primer! - (reply: 4)
  10. MSP gives smear only - (reply: 4)
  11. Infinium MEthylation Array Validation - (reply: 1)
  12. A question about MSP - (reply: 4)
  13. Amplicon size for BSP - (reply: 3)
  14. DNA methylation analysis - doubts - (reply: 5)
  15. Panomics Promoter Methylation PCR Kit and Methylation specific Digests - (reply: 2)
  16. methylated plasmids in culture - (reply: 1)
  17. Confirming published methylation primers - (reply: 1)
  18. Cloning with methylated vector - (reply: 2)
  19. Bisulfite PCR Question - (reply: 2)
  20. CpG island prediction - (reply: 3)
  21. complete bisulfite treatment - How do you know if your DNA is completely modified by bisulfite treatm (reply: 2)
  22. MSP is not reliable! - methodology discuss. (reply: 4)
  23. MethyISEQr Bisulfite Conversion kit from AB, is it good? - (reply: 4)
  24. How long does the methylation status of a gene last after AZA treatment? - (reply: 2)
  25. please help me check BSP primers. - (reply: 6)
  26. How to study promoter. - A presentation in related to DNA methylation (reply: 1)
  27. Please help me design BSP primer. - (reply: 14)
  28. Please help me analyze BSP cloned sequencing results. - (reply: 8)
  29. DNA Methylation Analysis - MeDIP (reply: 5)
  30. DNA quality for MSP? - (reply: 2)
  31. Bisulfite Sequencing: establishment - (reply: 1)
  32. DH5 alpha methylation profile - (reply: 8)
  33. WGA problems CpG island amplification - (reply: 6)
  34. need a whole genome methylation protocol - (reply: 2)
  35. Methylation studies - New to this field... help me (reply: 1)
  36. Problem with amplifying bisulfite treated DNA from FFPE tissue - (reply: 3)
  37. Inconsistent bisulfite PCR - (reply: 8)
  38. BSP to test imprinted genes DNA methylation of oocytes - no specific or smear bands! (reply: 4)
  39. Bisulfite tx'd DNA sequence analysis - what to do with CG to TA conversion - bisulfite sequencing analysis (reply: 7)
  40. Can bisulphite treatment be used for detection of DNA methylation within bacteri - (reply: 2)
  41. DNA methylation in leucocyte - (reply: 2)
  42. Duplex MSP? - (reply: 2)
  43. Wrong PCR product after Bisulfite Treatment-reason? - (reply: 1)
  44. Bisulfite Modification Efficiency - (reply: 5)
  45. Advice on analysing Differentially Methylated Regions of a gene. - (reply: 5)
  46. ready to use primers for BSP - (reply: 4)
  47. DNA methylation direct kit - (reply: 2)
  48. Negative control shows methylation? - (reply: 1)
  49. MSP and BSP confusion - a bit confusion about MSp (reply: 4)
  50. primer design for MSP and BSP - primers must be in promoter region? (reply: 1)
  51. Bisulfite sequencing - why cloning? - (reply: 3)
  52. Illumina Golden gate methylation? - (reply: 4)
  53. Nested PCR for BSP question - (reply: 4)
  54. MSP Primer design -rules to choose primers and reasons - (reply: 2)
  55. pGEM methylation status - (reply: 6)
  56. QPCR with MSP - (reply: 3)
  57. Bisulfite: PCR condition or primer problem? - (reply: 13)
  58. Nanog Methylation and sequencing - (reply: 3)
  59. Bisulfite sequencing of PCR products after AIMS technique - (reply: 3)
  60. tool for methylation - (reply: 1)
  61. Cloning for Bisulfite sequencing - TOPO TA no results...... pGEM???? (reply: 2)
  62. ELISA/Dot blot for detection of Methylated DNA - (reply: 3)
  63. TATA BOX AND CPG ISLANDS - (reply: 1)
  64. methylation, irreversible? - (reply: 2)
  65. Gene body methylation and active gene expression - (reply: 6)
  66. DNA Methylation - Tissue specific - (reply: 4)
  67. De novo plasmid methylation in eukaryotic cells - (reply: 3)
  68. type of detection, type of bisulfite conversion, =>different results? - variability between preps and such (reply: 9)
  69. Good or not?----MethylDetector™ Bisulfite Modification Kit - Could anyone help me evaluate this protocal for bisulfate treatment (reply: 2)
  70. Controls for Bisulfite Sequencing - (reply: 13)
  71. False positives? MSP and BGS - (reply: 9)
  72. bisulfite treatment - in my hands bisulfite kit don't work well (reply: 1)
  73. Bisulfte PCR and sequencing PCR and Primer Notes - Tips and hints on PCR for bisulfite converted DNA and bisulfite primer (reply: 33)
  74. Methylation of promoter-luciferase construct - investigate methylation on promoter activity (reply: 7)
  75. Use of W primers to detect efficiency of bisulfite reactions? - (reply: 5)
  76. Does anyone know any kind of array or chip for methylation scan? - (reply: 3)
  77. Nanodrop question - Spectrophotometrical measurment of bisulfite treated DNA (reply: 15)
  78. Direct sequencing on BSP PCR products: problems with A and T stretches - (reply: 3)
  79. Methyl Primer Express from ABI - (reply: 2)
  80. ChIP and CpG island microarrays - (reply: 1)
  81. MSP/USP in human tissue - USP not working! (reply: 3)
  82. General methylation question - (reply: 1)
  83. Primer design for BSP - (reply: 12)
  84. HMW band in well after bisulfite PCR - (reply: 1)
  85. 5ug DNA can be converted in one bisulfite treatment - (reply: 8)
  86. Problems with PCR following bisulfite treatment - (reply: 11)
  87. Heavy BSP bias towards methylated DNA - Why??? (reply: 5)
  88. Which CpG island to pick? - (reply: 1)
  89. where to find the list of genes known to be methylated - (reply: 2)
  90. No PCR Product after Bisulfite Modification - (reply: 5)
  91. How to transfer the BSP sequencing results into the standard legend? - (reply: 8)
  92. Bisulfite Sequencing Technique - (reply: 8)
  93. BSP question - (reply: 2)
  94. Genome-wide methylation analysis - (reply: 4)
  95. start a methylation study - (reply: 10)
  96. need help with methylation pattern - (reply: 5)
  97. MSP and Bisulfite genomic sequencing results don't match! - (reply: 5)
  98. DNA methylation and gene silencing - (reply: 5)
  99. methylated plasmid - (reply: 3)
  100. ABI have released MethylPrimer Express! - A new primer design program for DNA methylation....For Free!!! (reply: 45)
  101. Unique unspecific product using MSP - (reply: 3)
  102. Are transiently transfected DNA methylated in mammalian cells? - (reply: 3)
  103. Bisulfite sequencing primer - (reply: 4)
  104. MSP Primer Sets (Herman Papers) - (reply: 1)
  105. BSP, MSP - many different ways - (reply: 4)
  106. Methylated + bisulfite-treated DNA works better than w/o methylation - Desulfonation responsible? (reply: 8)
  107. DNA methylation vs gene expression - (reply: 3)
  108. BSP Primer placement over KNOWN unmethylated CpG's - Can this be done? (reply: 3)
  109. Bisulfite sequencing results - how to analyze (reply: 1)
  110. Bisulfite treatment? - (reply: 1)
  111. Positive Control for Methylation Studies in BSP sequencing - A "Universally" methylated Gene ?? (reply: 7)
  112. MSP - (reply: 1)
  113. MSP PCR - (reply: 2)
  114. Puzzle about methylation on plant microRNA - (reply: 1)
  115. Success with Direct Sequening of Bisulfite Pcr Products - (reply: 47)
  116. How long can Bisulfite treated DNA be stored? - (reply: 6)
  117. MSP buffer composition - (reply: 1)
  118. CpG calculation? - (reply: 3)
  119. MSP condition - (reply: 4)
  120. MSP trouble - (reply: 4)
  121. Trouble with primers of BSP - (reply: 3)
  122. DNA methylation content (degree) - (reply: 2)
  123. Two bands with MSP - (reply: 6)
  124. How to determine the DNA methylation of the whole gene - Primer design for BSP (reply: 18)
  125. Methylation Analysis-New methods? - (reply: 7)
  126. shorter PCR products after bisulfite treatment and cloning - (reply: 5)
  127. whole blood for dna methylation? - (reply: 2)
  128. How to eliminate primer dimer in MSP - (reply: 20)
  129. The sensitivity of MSP - (reply: 1)
  130. CpG island definition - (reply: 1)
  131. Nested MSP - (reply: 3)
  132. BSP - favors amplification of unmethylated template? - (reply: 2)
  133. Which polymerase for bisulfite sequencing PCR - (reply: 3)
  134. Starting to work with methylation - (reply: 1)
  135. Methylation and expression of imprinted genes - (reply: 3)
  136. global methylation level - (reply: 2)
  137. Mouse MSP primers - (reply: 11)
  138. question after getting BSP result - (reply: 2)
  139. HPLC protocol for methylation quantification - (reply: 21)
  140. Help!Checking the nested Primer - P15 gene nested MSP (reply: 2)
  141. How to determine the result of my msp? - methylated or unmethylated? (reply: 5)
  142. p16 methylation in pituitary adenomas - p16 in pituitary adenomas (reply: 2)
  143. What the hell is CpG island methylator phenotype (CIMP)? - (reply: 6)
  144. MSP result - (reply: 3)
  145. Which MSP & BGS primer design tool do you "swear" by? - (reply: 9)
  146. Which DNA fragment as control for complete bisulfite conversion - (reply: 6)
  147. MSP product size - (reply: 1)
  148. How to tell incomplete bisulfite treament from real methylation - (reply: 1)
  149. Does methylation matter? - (reply: 1)
  150. MSP question - (reply: 1)
  151. Allele specific cloning efficiency after bisulfite treatment - (reply: 4)
  152. Do you believe CpG methylation pattern? - (reply: 7)
  153. multiple products after MSP - (reply: 6)
  154. CpG methylation - (reply: 7)
  155. Southern blot v. bisulfite sequencing - which is better to detect methylation? (reply: 1)
  156. CpG methylation - Methylation on GpC also possible? (reply: 2)
  157. MSP primers not working - (reply: 1)
  158. problems with bisulfite PCR - (reply: 8)
  159. methylation sensitive restriction site - (reply: 2)
  160. About MSP result ! - why always have two bands (reply: 3)
  161. Non CpG cytosine methylation - Global methylation analysis of somatic tissue (reply: 1)
  162. Does it help to add antisense primer first for bisulfite PCR? - (reply: 2)
  163. Bisulfite treated DNA specific primer set critique? - (reply: 18)
  164. No product/primer dimers after BSP - (reply: 1)
  165. interesting flower - plant methylation (reply: 4)
  166. Bisulfite genomic sequencing - Bisulfite genomic sequencing (reply: 1)
  167. Unpurifed DNA for methylation - (reply: 1)
  168. One 'T' missing on one allele after bisulfite treatment - (reply: 8)
  169. bisufite treated products - (reply: 5)
  170. Primer for BSP - (reply: 1)
  171. BSP cycles and primers - Is it OK to use the same primer set but with more (reply: 6)
  172. Troubleshooting problem in PCR for bisulfite Treated DNA - (reply: 1)
  173. Does Temperature Alter DNA Methylation Status - Temperature and Methylation (reply: 1)
  174. Genes with no CpG islands... - (reply: 6)
  175. what is genomic DNA size after bisulfite treatment? - (reply: 3)
  176. Can these CpG islands be representative? - (reply: 1)
  177. Use of restriction enzyme digestion before bisulfite conversion - What is the purpose of r-enzyme digestion? (reply: 2)
  178. MSP bands - both in methylated and unmethylated DNA (reply: 3)
  179. General CpG island questions - (reply: 15)
  180. Help: about the MSP result ! - how to explain the My MSP result? (reply: 1)
  181. About MSP positive control - Choose what to methylate as positive control (reply: 5)
  182. Storage Of Bisulfite treated DNA - Storage conditions (reply: 2)
  183. Starting with DNA methylation analysis - (reply: 11)
  184. methylation in CpG island - (reply: 5)
  185. Why proofreading Taq can't be used in MSP - (reply: 6)
  186. Problems with MSP - Unable to pick up bands for methylation (reply: 1)
  187. Plasmid methylation problem - Plasmid methylation (reply: 3)
  188. Reading chromatograms after bisulfite sequencing - Bisulfite sequencing (reply: 7)
  189. Analysis of CpG islands and promoters for methylation study - (reply: 10)
  190. Different methylation with normal Taq and High fidelityTaq - Should I use non-fidelTaq instead of High fidelity (reply: 4)
  191. Bisulfite sequencing -- Positive control? - bifsulfite sequencing (reply: 3)
  192. DNA extraction for methylation - DNA from cell-free serum for methylation treatment (reply: 4)
  193. CpG islands in complement - (reply: 1)
  194. CpNpG - methylation or incomplete conversion? - (reply: 2)
  195. Why can the MSP represent the methylation of CpG island? - (reply: 3)
  196. Multiplex MSP? - (reply: 4)
  197. Folate And Methylation - (reply: 56)
  198. Designing methylation primers on long CpG island - (reply: 14)
  199. Bisulfite sequencing problems - T>C conversion (reply: 13)
  200. Define a CpG island - (reply: 2)
  201. bisulphite treatment for MSP - bisulphite treatment for MSP (reply: 7)
  202. Bisulfite sequencing problems - when CpG sites are too much... (reply: 4)
  203. Amplify long fragment from bisulfite treated template - for bisulfite sequencing (reply: 19)
  204. mC not in CpG? Primer selection - (reply: 1)
  205. Enzyme digestion before bisulfite treatment - (reply: 1)
  206. I want to learn "MSP" - (reply: 2)
  207. ranting about methylation analysis - (reply: 2)
  208. Starting methylation analysis, how! - (reply: 1)
  209. Primer design for MSP queries - (reply: 3)
  210. Is there a PCR length limitation after Bisulfite treatment? - (reply: 3)
  211. The actual bisulfite treatment - protocol and troubleshooting (reply: 58)
  212. PCR more difficult in methylated CpG region? - (reply: 7)
  213. Assessing methylation levels - not CpG specific but across CpG island (reply: 3)
  214. Negative control for MSP - (reply: 4)
  215. why don't do MSP product sequencing - (reply: 2)
  216. Problems with bisulfite modification - Problems with bisulfite modification (reply: 3)
  217. Methylation difficulties - (reply: 6)
  218. MSP - specificity - (reply: 2)
  219. Bisulfite sequencing - Does strand matter?? (reply: 3)
  220. positive control for MSP - (reply: 3)
  221. DNA hypo-methylation analysis - Check if a CpG island is hypomethylated (reply: 1)
  222. methylation primers - problems with primers (reply: 1)
  223. Methylation and restriction digestion problem - (reply: 8)
  224. Bisulfite modification kits and PCR - (reply: 5)
  225. PCR Cloning - primer design and CpG methylation (reply: 4)
  226. CpG Island length variation - How variable? (reply: 3)
  227. bisulfite pcr primers - (reply: 1)
  228. methylation specific PCR (MSP) - reproducibilty? (reply: 1)
  229. Software for MSP (methylation-specific PCR) primer design - (reply: 3)