methylated plasmid - (Jun/08/2006 )
I am trying to transfect a bacterium that is sensitive to methylation but my plasmids are from a bacterium that methylates. Can I transfect an E. coli that doesn't methylate plasmids and receive unmethylated plasmids?
many common used E.coli sgtrains have been modified not to have DNA methylase,such as DH5a,Top10,you can just try!Good luck!
Check out stratagene, invitrogen, neb for e. coli that doesn't methylate, most strains (the mentioned DH5a, Top10) do methylate, but there are some dam- and dcm- ones around.
links (probably other companies supply them as well):
Thanks for the help. I have found one from New England Biolabs