global methylation level - (Nov/24/2005 )

Hi,

Does anybody use 5-MeC antibody to detect global DNA methylation level?

Thanks,

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If u ask the search machine GOOGLE - vvv.google.com
u obtain 131 possible answers:
some examples


Modulation by celecoxib and difluoromethylornithine of the ...
(A) Representative dot blot analysis using the antibody to 5-MeC in DNA. ...
reverse global DNA hypomethylation implies that blocking DNA methylation is not ...
carcin.oxfordjournals.org/cgi/content/full/25/10/1917 - Panašūs puslapiai

Arsenic-induced DNA hypomethylation affects chromosomal ...
Then, the antibody against 5-methylcytosine (anti-5-MeC, Megabase Research Products,
... Correlation between DNA methylation levels and instability markers ...
carcin.oxfordjournals.org/cgi/content/full/25/3/413 - Panašūs puslapiai
[ Daugiau rezultatų iš carcin.oxfordjournals.org ]

Reduced levels of poly(ADP-ribosyl)ation result in chromatin ...
... individual cells after indirect immunolabeling with anti-5MeC antibodies. ...
Effect of Limited DNA Methylation Reprogramming in the Normal Sheep Embryo ...
www.fasebj.org/cgi/content/abstract/13/1/89 - Panašūs puslapiai

Reduced levels of poly(ADP-ribosyl)ation result in chromatin ...
This DNA methylation pattern, which is defined during embryonic development (18),
... The cells were then incubated with monoclonal anti-5MeC antibodies as ...
www.fasebj.org/cgi/content/full/13/1/89 - Panašūs puslapiai

[PDF] DNA demethylation is directly related to tumour progression ...
Dokumento formatas: PDF/Adobe Acrobat - HTML kopija
tissues was the first method used to evaluate the global. methylation status of
genomic DNA, giving an average value. of 5-MeC percentage in the whole ...
147.52.72.117/OR/2003/volume10/number3/545.pdf - Panašūs puslapiai


P.S.good luck - it's very important to obtain understanding about global methylation level when cancer research

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HPLC , HPCE and LC-MS are much more sensitive than using the anti 5-methylcytosine.

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