positive control for MSP - (Sep/16/2004 )

Hi there!

Like many of you, I´m also starting to make methylation spesific PCR, and I would like to know what is the best way to get totally methylated human DNA as a positive control for my PCRs?

I guess the negative control would be human sperm DNA.

Yours, Daisy

To get fully methylated DNA as a positive control, you can treat plasimd DNA with SssI methyltransferase which catalyzes de novo methylation on CpG sites. You can also purchase such DNA from commercial sources such as Chemicon.