CpNpG - methylation or incomplete conversion? - (Apr/15/2005 )
Hi
I know most methylation in humans occurs at CpG sites - but what about CpNpG sites. I ask because I have detected some C at such a site when I was expecting 100% T. I put this down to incomplete conversion, but have been reading around a bit and found several examples of this. I'd be interested in other peoples opinions, and of any published examples that people could refer me to.
Cheers
Jon
-spjgjsm-
QUOTE (spjgjsm @ Apr 15 2005, 07:55 AM)
Hi
I know most methylation in humans occurs at CpG sites - but what about CpNpG sites. I ask because I have detected some C at such a site when I was expecting 100% T. I put this down to incomplete conversion, but have been reading around a bit and found several examples of this. I'd be interested in other peoples opinions, and of any published examples that people could refer me to.
Cheers
Jon
I know most methylation in humans occurs at CpG sites - but what about CpNpG sites. I ask because I have detected some C at such a site when I was expecting 100% T. I put this down to incomplete conversion, but have been reading around a bit and found several examples of this. I'd be interested in other peoples opinions, and of any published examples that people could refer me to.
Cheers
Jon
Jon,
that depends on what organism you are looking in. Sure in plants CpNpG methylation is quite common and achieved by chromomethylase. This has also been described in humans, though I have not seen it with my studies.
Nick
-methylnick-
I second Nick. It has been reported in human but not confirmed yet. Most likely an artfact.
-pcrman-