methylation sensitive restriction site - (Aug/31/2005 )
I have to cut the XbaI site in pEGFP-C1 where the restriction site is methylation sensitive. The plasmid DNA is miniprep from DH5a strain. What kinds of competent cell should I select for transformation to get the demethylated DNA?
E coli JM110 cells are dam- and dcm-
One shot from invitrogen works great...