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Suppression Subtractive Hybridization for methylation study - (Mar/16/2005 )


when i read journals about SSH on differentially methylated sequences between two pools of dna, all of them use a marker called "haeII-digested phiX174 DNA marker. Why is this marker so special ? Can I use a normal DNA marker instead, like GENERULER 1kb DNA marker? THanks



u could use other marker. to my limted knowledge, phi174/HaeII marker
was used just as they have used it as one control in mock exp.
Nothing else special .