MethyISEQr Bisulfite Conversion kit from AB, is it good? - (Jun/02/2008 )

I have never touched methylation analysis before. My boss threw me a project to analyze methylation status of a promoter region. I have the MethyISEQr Bisulfite Conversion kit from Applied Biosystems. But I find few people here discussed this kit. Is it good? Does any people here have experience with this kit?
In additon, many protocols require to digest your DNA with appropriate restriction enzyme at the very beginning. But the MethyISEQr Bisulfite kit protocol doesn't need to do this. Is it normal?

Thanks

-macrosky-

QUOTE (macrosky @ Jun 2 2008, 06:32 PM)

For bisulfite conversion, ZYMO Research methylation kit I used is very easy. For methylation analysis, you can use sybr green -based qMSP.

-larryking-

I have used the Qiagen Epitect kit and had plenty of success with it, and this doesn't involve a digestion step beforehand.
Just give the kit a go and see if it works for you. If you are convinced that your PCR conditions and primers are good and have no amplification perhaps then you could try another kit.

-Davo-

QUOTE (macrosky @ Jun 2 2008, 02:32 PM)

In additon, many protocols require to digest your DNA with appropriate restriction enzyme at the very beginning. But the MethyISEQr Bisulfite kit protocol doesn't need to do this. Is it normal?

Indeed you do not require shearing or pre-digestion of genomic DNA prior to the use of most conversion kits.

good luck!

Nick

-methylnick-

sneth approves this kit.

methyl conversion kits these days are all pretty standard.

-sneth-