Methylated cytosine nucleotide - (Jun/19/2007 )
Does anyone kwnow where to order a methylated cytosine nucleotide? dCTP-methylated?
I believe Roche makes it. You should be aware that the melting temperature of G - 5m-C bonds is higher than GC bonds, and you may need to adjust the annealing temperature and denaturing temperature of your PCR reaction to compensate. There seems to be no agreement on the name of this chemical, or its abbreviation, so you probably have to try many different ones. I think Epicentre might also supply it.