The sensitivity of MSP - (Dec/27/2005 )
Hi,pcrman,now I have been doing the MSP study.I have got beautiful bands with primers designed
by Methprimer in the positive and negative cell line.The next step i want to do is to check out the
sensitive of the MSP.Could you tell me how to do ?Or What i should do next after I got the bands
I want? Thank you very much!!
Do you mean you want to confirm whether the MSP results are true?
I think for every new pair of MSP primers, the result has to be confirmed by bisulfite sequencing using BSP primers that cover the MSP region.
>>What i should do next after I got the bands
The bands already tell you the methylation status of your samples. bands from M pair means methylation, from U pair unmethylation.