Storage Of Bisulfite treated DNA - Storage conditions (Jun/20/2005 )

Hi,
I would like to know about Storage conditions for Bisufite treated DNA for long periods of time ( around six months). I treated the DNA by using Ez DNA methylation kit and i diluted the treated DNA for PCR amplification with sterile Water for ten times. So far i came across methods for storing the treated DNA , like (1)Resuspending the treated DNA in TE buffer (2) Storing the treated DNA in 20-100 µL of sterile 1mM Tris-cl with pH8 at -20°C etc. But i want to know about some more methods that are used sucessfully for storing Bisufite treated DNA diluted with sterile water and also for non diluted Bisulphite treated DNA.

Ravi

Hi Ravi,

I think both should work. Some of my colleagues even leave modified DNA at 4C without problem. I have used TE (10 mM Tris-HCl, 0.1 mM EDTA, pH 7.5) or EB (10 mM Tris-HCl, pH8.0) or water. Just make sure they don't interfere downstream PCR reaction. I always store my DNA at -20C.

Water is not the ideal solution to dissolve DNA as it is not buffered and variations in pH will lead to DNA instability and degradation.

I tend to stick with TE as it is buffered and the EDTA should prevent any enzymatic degradation should you get contamination.

Good luck!

Nick