Storage Of Bisulfite treated DNA - Storage conditions (Jun/20/2005 )
I would like to know about Storage conditions for Bisufite treated DNA for long periods of time ( around six months). I treated the DNA by using Ez DNA methylation kit and i diluted the treated DNA for PCR amplification with sterile Water for ten times. So far i came across methods for storing the treated DNA , like (1)Resuspending the treated DNA in TE buffer (2) Storing the treated DNA in 20-100 µL of sterile 1mM Tris-cl with pH8 at -20°C etc. But i want to know about some more methods that are used sucessfully for storing Bisufite treated DNA diluted with sterile water and also for non diluted Bisulphite treated DNA.
I think both should work. Some of my colleagues even leave modified DNA at 4C without problem. I have used TE (10 mM Tris-HCl, 0.1 mM EDTA, pH 7.5) or EB (10 mM Tris-HCl, pH8.0) or water. Just make sure they don't interfere downstream PCR reaction. I always store my DNA at -20C.
Water is not the ideal solution to dissolve DNA as it is not buffered and variations in pH will lead to DNA instability and degradation.
I tend to stick with TE as it is buffered and the EDTA should prevent any enzymatic degradation should you get contamination.