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dissociation curves - (Aug/06/2013 )

Please find the attached files for dissocoation curves from qpcr 

 do they look fine????

please suggest if they are not good.


thanx in advance

Attached File


No. That is typical of an instrument issue. You also have some high temp peaks indicative of mis-priming. That's usually due to primer design. Also, your Supermix can cause problems if its not ideal. Check out this tutorial for Supermix help.