0.5% crystal violet - how to make (Aug/19/2009 )
Hi,
I have to make 0.5% crystal violet in 25% methanol. So, if I understand correct is the following calculation corect for making 10ml total volme:
1) 0.05g crystal violet
2) 2.5ml 25% methanol
3) 7.5ml water.
Or do i have to dissolve the crystal violet in 10ml of 25%methanol?
-SF_HK-
SF_HK on Aug 19 2009, 05:03 PM said:
Hi,
I have to make 0.5% crystal violet in 25% methanol. So, if I understand correct is the following calculation corect for making 10ml total volme:
1) 0.05g crystal violet
2) 2.5ml 25% methanol
3) 7.5ml water.
Or do i have to dissolve the crystal violet in 10ml of 25%methanol?
I have to make 0.5% crystal violet in 25% methanol. So, if I understand correct is the following calculation corect for making 10ml total volme:
1) 0.05g crystal violet
2) 2.5ml 25% methanol
3) 7.5ml water.
Or do i have to dissolve the crystal violet in 10ml of 25%methanol?
You need to add 0.05g crystal violet + 2.5ml 100% methanol + 7.5ml water. This way you'll have 0.5% crytal violet in 25% methanol.
Your calculations give 0.625% methanol.
-almost a doctor-
Dissolve crystal violet in methanol first, then add DW.
-genehunter-