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What happen to my northern blot? - (Dec/04/2010 )

What might be the problem if my northern blots show very weak signals or have lots of smeary stuff?


transfer problems, running conditions, RNase presence, low radio-nucleide incorporation (or DIG if you are using it), not enough substrate (DIG), annealing temp too high or too low... etc,

Pretty much anything. Can you post your protocol?