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Do you know good sequencing companies? - Sanger sequencing (Mar/04/2011 )

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Hi there,

I was wondering if you have a favorite sequencing company? I am specifically looking for companies that do Sanger sequencing (for full 16s rDNA). However, there are so many companies out there that it is a bit overwhelming. So I thought I'll check what your opinions are. Please let me know if there is a company you trust. Thanks!

Cheers,
Verena

-Wolverena-

Macrogen

-BioMiha-

I would prefere a company that is somehow near your lab (if possible on the same continent) ...so you do not have to send samples all around the world (allthough some people do send samples for sanger sequencing e.g. from Europe to Korea and that is a bit weird to me). Normally, sanger sequencing is a state of the art technique and read lengths >1000bp are more or less standard ...i think it is most important to purify the DNA in a good way because when it is of poor quality the sequencing will fail no matter what company you have choosen.

Regards,
p

BioMiha on Sat Mar 5 11:47:24 2011 said:


Macrogen

-pDNA-

Source BioScience are good in Europe, they have labs in UK, Irelan and Germany. Where are you?

-DrDocter-

pDNA on Sun Mar 6 11:27:02 2011 said:


I would prefere a company that is somehow near your lab (if possible on the same continent) ...so you do not have to send samples all around the world (allthough some people do send samples for sanger sequencing e.g. from Europe to Korea and that is a bit weird to me). Normally, sanger sequencing is a state of the art technique and read lengths >1000bp are more or less standard ...i think it is most important to purify the DNA in a good way because when it is of poor quality the sequencing will fail no matter what company you have choosen.

Regards,
p

BioMiha on Sat Mar 5 11:47:24 2011 said:


Macrogen



I agree with you, but we use macrogen too.. just because its soo cheap.. Altough I dont agree with it because the impact (CO2, airplance etc) is too big. But, money still rules ... and sending it to macrogen still stays very cheap <_<

-pito-

how cheap is it? .../per run?

Regards,
p

pito on Mon Mar 7 12:51:27 2011 said:


pDNA on Sun Mar 6 11:27:02 2011 said:


I would prefere a company that is somehow near your lab (if possible on the same continent) ...so you do not have to send samples all around the world (allthough some people do send samples for sanger sequencing e.g. from Europe to Korea and that is a bit weird to me). Normally, sanger sequencing is a state of the art technique and read lengths >1000bp are more or less standard ...i think it is most important to purify the DNA in a good way because when it is of poor quality the sequencing will fail no matter what company you have choosen.

Regards,
p

BioMiha on Sat Mar 5 11:47:24 2011 said:


Macrogen



I agree with you, but we use macrogen too.. just because its soo cheap.. Altough I dont agree with it because the impact (CO2, airplance etc) is too big. But, money still rules ... and sending it to macrogen still stays very cheap <_<

-pDNA-

4/run, free shipping for over 20 samples

-BioMiha-

pito on Mon Mar 7 12:51:27 2011 said:


pDNA on Sun Mar 6 11:27:02 2011 said:

I would prefere a company that is somehow near your lab (if possible on the same continent) ...so you do not have to send samples all around the world (allthough some people do send samples for sanger sequencing e.g. from Europe to Korea and that is a bit weird to me). Normally, sanger sequencing is a state of the art technique and read lengths >1000bp are more or less standard ...i think it is most important to purify the DNA in a good way because when it is of poor quality the sequencing will fail no matter what company you have choosen. Regards,p

BioMiha on Sat Mar 5 11:47:24 2011 said:

Macrogen
I agree with you, but we use macrogen too.. just because its soo cheap.. Altough I dont agree with it because the impact (CO2, airplance etc) is too big. But, money still rules ... and sending it to macrogen still stays very cheap <_<

As for the impact. I'm pretty sure there are easier ways to help our planet than to stop shipping samples to Korea?

-BioMiha-

for sure there are better ways ...i agree with you!
...but you know things add up!!! Sequencing from Korea, Apples from Chile, Bananas from Costa Rica, Clothes from China, Shoes from Vietnam and so on! I know we live in a globalized world ...if we want to stop pollution/climate change we have to overthink our habits.

Okay, i pay 0.5 more per run ...but therefore have a lower carbon footprint since i send my samples 500 km instead of 10000 km.
Think about that ...and how many researchers are on this planet ...and if everyone sends his samples all around the world just because of the mony and due to the fact that someone does it cheaper ...where we will end up?

So, i do not blame Macrogen ...it is for sure a good company ...and i like Korea ...so it has nothing to do with that ...just to make that clear :)

Regards,
p


As for the impact. I'm pretty sure there are easier ways to help our planet than to stop shipping samples to Korea?

-pDNA-

BioMiha on Mon Mar 7 20:36:02 2011 said:


pito on Mon Mar 7 12:51:27 2011 said:


pDNA on Sun Mar 6 11:27:02 2011 said:

I would prefere a company that is somehow near your lab (if possible on the same continent) ...so you do not have to send samples all around the world (allthough some people do send samples for sanger sequencing e.g. from Europe to Korea and that is a bit weird to me). Normally, sanger sequencing is a state of the art technique and read lengths >1000bp are more or less standard ...i think it is most important to purify the DNA in a good way because when it is of poor quality the sequencing will fail no matter what company you have choosen. Regards,p

BioMiha on Sat Mar 5 11:47:24 2011 said:

Macrogen
I agree with you, but we use macrogen too.. just because its soo cheap.. Altough I dont agree with it because the impact (CO2, airplance etc) is too big. But, money still rules ... and sending it to macrogen still stays very cheap <_<

As for the impact. I'm pretty sure there are easier ways to help our planet than to stop shipping samples to Korea?


No... if you keep thinking like that, then you can justify everything....

Really, do you think its normal to send a package so many miles away just to save a buck? Its not only the airplane... its everything..
Its the mentality in general.
+ you keep supporting the fact that they have cheaper workers over there....

Its not amazing that there isnt a company in europe that can do it for the same price...

-pito-
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