Protocol Online logo
Top : New Forum Archives (2009-): : Molecular Biology

Co-immunoprecipitation - (Aug/14/2011 )

Hi there,

I'll be doing a co-inmunoprecipitation, and I would like to know 2 things:
- what concentration of bead is used in relation to amount of protein? And
- what amount of antibody is used for the concentration of bead used?



20 ul of beads with 1-1.5 ug of antibody