Analyzing qPCR results - (Feb/08/2010 )
Hi all,
I am doing qPCR to look at whether this gene is upregulated or downregulated in WT and Mut mice. How do I calculate the relative gene expression after i calculated the Ct value for the mutant and wild type?
THanks !!!
-serundipity2009-
serundipity2009 on Feb 8 2010, 10:11 AM said:
Hi all,
I am doing qPCR to look at whether this gene is upregulated or downregulated in WT and Mut mice. How do I calculate the relative gene expression after i calculated the Ct value for the mutant and wild type?
THanks !!!
I am doing qPCR to look at whether this gene is upregulated or downregulated in WT and Mut mice. How do I calculate the relative gene expression after i calculated the Ct value for the mutant and wild type?
THanks !!!
using analyse type: relative quantification in the lightcycler menu
-susanna-