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Analyzing qPCR results - (Feb/08/2010 )

Hi all,
I am doing qPCR to look at whether this gene is upregulated or downregulated in WT and Mut mice. How do I calculate the relative gene expression after i calculated the Ct value for the mutant and wild type?

THanks !!!

-serundipity2009-

serundipity2009 on Feb 8 2010, 10:11 AM said:

Hi all,
I am doing qPCR to look at whether this gene is upregulated or downregulated in WT and Mut mice. How do I calculate the relative gene expression after i calculated the Ct value for the mutant and wild type?

THanks !!!



using analyse type: relative quantification in the lightcycler menu

-susanna-