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20x SSC - northern transfer (Nov/15/2009 )

plz let me know
why is 20x SSC buffer used in the transfer of nucleic acids-why is a high strength buffer used?
thanks in advance

-ashpra-

ashpra on Nov 16 2009, 01:19 PM said:

plz let me know
why is 20x SSC buffer used in the transfer of nucleic acids-why is a high strength buffer used?
thanks in advance



well i ve never done a norhern blot but my concept says higher salt.. better diffusion.. lesser time for blotting!!!

-Pradeep Iyer-

Pradeep Iyer on Nov 16 2009, 12:35 AM said:

ashpra on Nov 16 2009, 01:19 PM said:

plz let me know
why is 20x SSC buffer used in the transfer of nucleic acids-why is a high strength buffer used?
thanks in advance



well i ve never done a norhern blot but my concept says higher salt.. better diffusion.. lesser time for blotting!!!


thank u for the reply but I think your answer defies the principle of diffusion...

-ashpra-

ooooops i meant capillary.. sorry for tat!!!

-Pradeep Iyer-