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quantifying the activity of a transcription factor - (Oct/21/2012 )

I am working with a transcription factor & i need to identify its activity among different time points. is there a method which can do that?

-mihoz-

You probably need some sort of reporter assay based on a known downstream target. This is usually done with luciferase assays.

-bob1-

i am sorry i needed to be more specific... i need an in vitro test not an in vivo one.

-mihoz-

Try in vitro transcription with rabbit reticulocyte lysate.

-bob1-