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qPCR multiplex- impact of the fluorophore on probe annealing temperature - (Mar/08/2013 )

Hi all,

Does anyone knows if the annealing temperature of probes is altered if an fluorophore and/or a quencher is link to the probe? Does all fluorophore has the same impact?

Thanks

-dan_-

Run a PCR and check

-jerryshelly1-