Protocol Online logo
Top : New Forum Archives (2009-): : Molecular Biology

In-situ hybridization - (Mar/12/2013 )

Could someone tell why do we do in situ hybridization? Is it only for finding information regarding localization of gene expression in tissue and to find mRNA content in the tissue?

-Mad researcher-

In situ hybridization is used for specific mRNA localization in tissue and a method of tissue specific expression for a gene.
Very useful for promotor studies as you have no need to tag your gene with gus or other sequence, then no further transformation in model plant and screening and then expression studies.
You can apply direct and save your 1-2 years time with In situ hybridization.
Good luck

Dr. Rupesh Kumar Singh
PhD (Plant Biotechnology)
Department of Biotechnology
Kumaun University, Nainital,
Uttarakhand, India-263139
Mob- +91-9911896375

-DrRupesh Kumar Rajput-