ampicillin for LB agar - (Mar/11/2010 )
hi, i want to make 8 LB Agar plates, with 100ug/ml of ampicillin in each plate. i have 63mg of ampicillin and i am not sure how to dilute and aliqout the ampicillin to the desired concentration. am going to prepare 500ml of LB Agar. so how do i prepare ampicillin to get 100ug/ml per plate and how much do i have to administer into the 500ml LB Agar? appreciate it if anyone can help. thanks!
azazel on Mar 12 2010, 10:21 AM said:
Dissolve 50mg of amp in 500uL of MQ and add 500ul of this antibiotic to 500mL Agar.
okay, thanks so much for the info!