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Top : New Forum Archives (2009-): : Molecular-Cloning
511. Pls help me with this plasmid!!! - (reply: 1)
512. Problems with Restriction Digest Results - (reply: 7)
513. Methods of plasmid transformation? only heat shock? - (reply: 1)
514. Digestion - (reply: 2)
515. Digestion of Genomic DNA - (reply: 1)
516. Isolation of a Gene of Interest for Subcloning - (reply: 8)
517. E.coli grow on neg. control - (reply: 1)
518. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
519. Need help troubleshooting digest. - (reply: 1)
520. Competent cells question: JM101 vs DH5alpha - (reply: 2)
521. How to avoid plasmid instability - (reply: 4)
522. Question Help - (reply: 1)
523. Shear-induced damage of plasmid by vortexing - (reply: 1)
524. Problems with blunt end ligation - (reply: 3)
525. Problem with Transforming E. coli DH10Bac - (reply: 8)
526. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
527. Heat inactivate ligase - (reply: 5)
528. DMSO stock of overnight bacterial cultures - (reply: 1)
529. Recommended competent cell for transformation of large plasmids? - (reply: 4)
530. Prolem in screening of hygromycin concentration - (reply: 1)
531. Primers for Introduction of new restriction sites to a vector - (reply: 3)
532. problem in cloning involving partial digestion - (reply: 1)
533. Why perform restriction enzyme digestion again? - (reply: 1)
534. Cotransient Transfections DNA Requirements - (reply: 3)
535. which software to draw the scheme of cloning strategy - (reply: 5)
536. Help with restriction analysis - (reply: 1)
537. Sequencing of PCR product - (reply: 4)
538. KanR sequence - (reply: 2)
539. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
540. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)