Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
511. TOPO TA Cloning Troubleshooting - (reply: 1)
512. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
513. Tricistronic vector - (reply: 1)
514. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
515. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
516. Directions of genes expression illustrations - (reply: 5)
517. Plasmid DNA at RT for a few days - (reply: 4)
518. TOPO TA Cloning - (reply: 1)
519. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
520. No insert in subcloning - (reply: 3)
521. Digest does not confirm presence of fragment - (reply: 5)
522. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
523. pGL3 Promoter replacing TSS bases - (reply: 1)
524. lipofectamine 2000:DNA complex stability - (reply: 2)
525. Problems with Ecoli glycerol stock - (reply: 6)
526. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
527. CIAP dilution - (reply: 5)
528. Problems in RNAi construction by using pUC19 - (reply: 3)
529. Unable to clone in E. coli cells - (reply: 3)
530. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
531. plasmid rearrangement or sabotage - (reply: 2)
532. pLDR20 Vector - (reply: 1)
533. surprising behavior of transformants needs immediate advice! - (reply: 8)
534. cloning antisense promoter in pGL3Basic - (reply: 2)
535. PhiX174 DNA - (reply: 1)
536. Competent bacterial cells - (reply: 2)
537. Replacing puramycin in a pLKO1.puro - (reply: 1)
538. colony cracking for screening of positive clones - (reply: 2)
539. deletion of large fregments - (reply: 3)
540. Linearising plasmid before transfection? - (reply: 2)