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Top : New Forum Archives (2009-): : Molecular-Cloning
511. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
512. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
513. Directions of genes expression illustrations - (reply: 5)
514. Plasmid DNA at RT for a few days - (reply: 4)
515. TOPO TA Cloning - (reply: 1)
516. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
517. No insert in subcloning - (reply: 3)
518. Digest does not confirm presence of fragment - (reply: 5)
519. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
520. pGL3 Promoter replacing TSS bases - (reply: 1)
521. lipofectamine 2000:DNA complex stability - (reply: 2)
522. Problems with Ecoli glycerol stock - (reply: 6)
523. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
524. CIAP dilution - (reply: 5)
525. Problems in RNAi construction by using pUC19 - (reply: 3)
526. Unable to clone in E. coli cells - (reply: 3)
527. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
528. plasmid rearrangement or sabotage - (reply: 2)
529. pLDR20 Vector - (reply: 1)
530. surprising behavior of transformants needs immediate advice! - (reply: 8)
531. cloning antisense promoter in pGL3Basic - (reply: 2)
532. PhiX174 DNA - (reply: 1)
533. Competent bacterial cells - (reply: 2)
534. Replacing puramycin in a pLKO1.puro - (reply: 1)
535. colony cracking for screening of positive clones - (reply: 2)
536. deletion of large fregments - (reply: 3)
537. Linearising plasmid before transfection? - (reply: 2)
538. Smear in plasmid sample - (reply: 9)
539. about a book - (reply: 1)
540. Cloning Problem! I need help - (reply: 3)