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Top : New Forum Archives (2009-): : Molecular-Cloning
511. Digest does not confirm presence of fragment - (reply: 5)
512. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
513. pGL3 Promoter replacing TSS bases - (reply: 1)
514. lipofectamine 2000:DNA complex stability - (reply: 2)
515. Problems with Ecoli glycerol stock - (reply: 6)
516. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
517. CIAP dilution - (reply: 5)
518. Problems in RNAi construction by using pUC19 - (reply: 3)
519. Unable to clone in E. coli cells - (reply: 3)
520. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
521. plasmid rearrangement or sabotage - (reply: 2)
522. pLDR20 Vector - (reply: 1)
523. surprising behavior of transformants needs immediate advice! - (reply: 8)
524. cloning antisense promoter in pGL3Basic - (reply: 2)
525. PhiX174 DNA - (reply: 1)
526. Competent bacterial cells - (reply: 2)
527. Replacing puramycin in a pLKO1.puro - (reply: 1)
528. colony cracking for screening of positive clones - (reply: 2)
529. deletion of large fregments - (reply: 3)
530. Linearising plasmid before transfection? - (reply: 2)
531. Smear in plasmid sample - (reply: 9)
532. about a book - (reply: 1)
533. Cloning Problem! I need help - (reply: 3)
534. Help designing primers for use in In-fushion cloning - (reply: 4)
535. Repeat of desired mutation in cloning product - (reply: 1)
536. vector dephosphorylation - (reply: 5)
537. ura3 mutant yeast strain grows on SD-uracil- - (reply: 1)
538. DH10BAC - (reply: 3)
539. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
540. protein is not expessing in HEK 293 cells - (reply: 2)