Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
511. How to get pure protein of a gene - (reply: 3)
512. How to insert genes into chromosome of E. coli - (reply: 1)
513. Gene synthesis - (reply: 2)
514. I canīt obtain the amount of DNA required for ligation of blunt ends - (reply: 2)
515. Pls help me with this plasmid!!! - (reply: 1)
516. Problems with Restriction Digest Results - (reply: 7)
517. Methods of plasmid transformation? only heat shock? - (reply: 1)
518. Digestion - (reply: 2)
519. Digestion of Genomic DNA - (reply: 1)
520. Isolation of a Gene of Interest for Subcloning - (reply: 8)
521. E.coli grow on neg. control - (reply: 1)
522. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
523. Need help troubleshooting digest. - (reply: 1)
524. Competent cells question: JM101 vs DH5alpha - (reply: 2)
525. How to avoid plasmid instability - (reply: 4)
526. Question Help - (reply: 1)
527. Shear-induced damage of plasmid by vortexing - (reply: 1)
528. Problems with blunt end ligation - (reply: 3)
529. Problem with Transforming E. coli DH10Bac - (reply: 8)
530. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
531. Heat inactivate ligase - (reply: 5)
532. DMSO stock of overnight bacterial cultures - (reply: 1)
533. Recommended competent cell for transformation of large plasmids? - (reply: 4)
534. Prolem in screening of hygromycin concentration - (reply: 1)
535. Primers for Introduction of new restriction sites to a vector - (reply: 3)
536. problem in cloning involving partial digestion - (reply: 1)
537. Why perform restriction enzyme digestion again? - (reply: 1)
538. Cotransient Transfections DNA Requirements - (reply: 3)
539. which software to draw the scheme of cloning strategy - (reply: 5)
540. Help with restriction analysis - (reply: 1)