Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
511. Problems with Ecoli glycerol stock - (reply: 6)
512. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
513. CIAP dilution - (reply: 5)
514. Problems in RNAi construction by using pUC19 - (reply: 3)
515. Unable to clone in E. coli cells - (reply: 3)
516. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
517. plasmid rearrangement or sabotage - (reply: 2)
518. pLDR20 Vector - (reply: 1)
519. surprising behavior of transformants needs immediate advice! - (reply: 8)
520. cloning antisense promoter in pGL3Basic - (reply: 2)
521. PhiX174 DNA - (reply: 1)
522. Competent bacterial cells - (reply: 2)
523. Replacing puramycin in a pLKO1.puro - (reply: 1)
524. colony cracking for screening of positive clones - (reply: 2)
525. deletion of large fregments - (reply: 3)
526. Linearising plasmid before transfection? - (reply: 2)
527. Smear in plasmid sample - (reply: 9)
528. about a book - (reply: 1)
529. Cloning Problem! I need help - (reply: 3)
530. Help designing primers for use in In-fushion cloning - (reply: 4)
531. Repeat of desired mutation in cloning product - (reply: 1)
532. vector dephosphorylation - (reply: 5)
533. ura3 mutant yeast strain grows on SD-uracil- - (reply: 1)
534. DH10BAC - (reply: 3)
535. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
536. protein is not expessing in HEK 293 cells - (reply: 2)
537. Restriction problems - (reply: 4)
538. cloning a gene in to a plasmid - (reply: 5)
539. no product on gel after restriction digestion - (reply: 3)
540. Gateway technology. - (reply: 4)