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Top : New Forum Archives (2009-): : Molecular-Cloning
511. Tricistronic vector - (reply: 1)
512. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
513. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
514. Directions of genes expression illustrations - (reply: 5)
515. Plasmid DNA at RT for a few days - (reply: 4)
516. TOPO TA Cloning - (reply: 1)
517. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
518. No insert in subcloning - (reply: 3)
519. Digest does not confirm presence of fragment - (reply: 5)
520. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
521. pGL3 Promoter replacing TSS bases - (reply: 1)
522. lipofectamine 2000:DNA complex stability - (reply: 2)
523. Problems with Ecoli glycerol stock - (reply: 6)
524. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
525. CIAP dilution - (reply: 5)
526. Problems in RNAi construction by using pUC19 - (reply: 3)
527. Unable to clone in E. coli cells - (reply: 3)
528. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
529. plasmid rearrangement or sabotage - (reply: 2)
530. pLDR20 Vector - (reply: 1)
531. surprising behavior of transformants needs immediate advice! - (reply: 8)
532. cloning antisense promoter in pGL3Basic - (reply: 2)
533. PhiX174 DNA - (reply: 1)
534. Competent bacterial cells - (reply: 2)
535. Replacing puramycin in a pLKO1.puro - (reply: 1)
536. colony cracking for screening of positive clones - (reply: 2)
537. deletion of large fregments - (reply: 3)
538. Linearising plasmid before transfection? - (reply: 2)
539. Smear in plasmid sample - (reply: 9)
540. about a book - (reply: 1)