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Top : New Forum Archives (2009-): : Molecular-Cloning
511. pGL3 Promoter replacing TSS bases - (reply: 1)
512. lipofectamine 2000:DNA complex stability - (reply: 2)
513. Problems with Ecoli glycerol stock - (reply: 6)
514. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
515. CIAP dilution - (reply: 5)
516. Problems in RNAi construction by using pUC19 - (reply: 3)
517. Unable to clone in E. coli cells - (reply: 3)
518. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
519. plasmid rearrangement or sabotage - (reply: 2)
520. pLDR20 Vector - (reply: 1)
521. surprising behavior of transformants needs immediate advice! - (reply: 8)
522. cloning antisense promoter in pGL3Basic - (reply: 2)
523. PhiX174 DNA - (reply: 1)
524. Competent bacterial cells - (reply: 2)
525. Replacing puramycin in a pLKO1.puro - (reply: 1)
526. colony cracking for screening of positive clones - (reply: 2)
527. deletion of large fregments - (reply: 3)
528. Linearising plasmid before transfection? - (reply: 2)
529. Smear in plasmid sample - (reply: 9)
530. about a book - (reply: 1)
531. Cloning Problem! I need help - (reply: 3)
532. Help designing primers for use in In-fushion cloning - (reply: 4)
533. Repeat of desired mutation in cloning product - (reply: 1)
534. vector dephosphorylation - (reply: 5)
535. ura3 mutant yeast strain grows on SD-uracil- - (reply: 1)
536. DH10BAC - (reply: 3)
537. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
538. protein is not expessing in HEK 293 cells - (reply: 2)
539. Restriction problems - (reply: 4)
540. cloning a gene in to a plasmid - (reply: 5)