Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
511. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
512. Another cloning hell....no transformants - (reply: 1)
513. Agrobacterium LBA4404 transformation - (reply: 1)
514. TOPO TA Cloning Troubleshooting - (reply: 1)
515. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
516. Tricistronic vector - (reply: 1)
517. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
518. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
519. Directions of genes expression illustrations - (reply: 5)
520. Plasmid DNA at RT for a few days - (reply: 4)
521. TOPO TA Cloning - (reply: 1)
522. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
523. No insert in subcloning - (reply: 3)
524. Digest does not confirm presence of fragment - (reply: 5)
525. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
526. pGL3 Promoter replacing TSS bases - (reply: 1)
527. lipofectamine 2000:DNA complex stability - (reply: 2)
528. Problems with Ecoli glycerol stock - (reply: 6)
529. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
530. CIAP dilution - (reply: 5)
531. Problems in RNAi construction by using pUC19 - (reply: 3)
532. Unable to clone in E. coli cells - (reply: 3)
533. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
534. plasmid rearrangement or sabotage - (reply: 2)
535. pLDR20 Vector - (reply: 1)
536. surprising behavior of transformants needs immediate advice! - (reply: 8)
537. cloning antisense promoter in pGL3Basic - (reply: 2)
538. PhiX174 DNA - (reply: 1)
539. Competent bacterial cells - (reply: 2)
540. Replacing puramycin in a pLKO1.puro - (reply: 1)