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Top : New Forum Archives (2009-): : Molecular-Cloning
511. E. coli growing on plates and bot not in LB medium - (reply: 10)
512. *** Human Insulin promoter sequence*** - (reply: 2)
513. digested plasmid - (reply: 2)
514. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
515. Subcloning - RE digest and ligation and transformation - (reply: 5)
516. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
517. Making DNA Linkers for cloning purposes - (reply: 1)
518. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
519. Dam-/+ e. coli strains - (reply: 6)
520. cloning short genomic DNA fragments - (reply: 1)
521. finding the protein and nucleotide matches - (reply: 1)
522. DH10B with big construct question - (reply: 2)
523. QIAGEN Spin Columns and Minipreps - Alternatives?! - (reply: 15)
524. cloning to pET28a - (reply: 1)
525. Weird Library Transformation Results - (reply: 10)
526. Injecting mice with lentivirus - (reply: 1)
527. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
528. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)
529. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
530. Ligation into a BAC? - (reply: 2)
531. oligo continually appears less on gel compared to other same load - (reply: 2)
532. Is it possible to make your own Rosetta(DE3) pLysS by transforming pLysS-Rosetta - (reply: 1)
533. plasmid tetR and tet promoter - (reply: 2)
534. unable to amplify an infectious retrovirus clone - (reply: 4)
535. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
536. No bands at all after double digestion - (reply: 1)
537. Digest for insert orientation - (reply: 4)
538. Colonies from transformation plate are not growing...:( - (reply: 5)
539. Problem with ligating a small insert (LoxP site) - (reply: 5)
540. Is the genomic DNA similar among different cell lines? - (reply: 4)