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511. Help! Problems with SDM with QUikChange II XL site-directed mutagenesis kit - (reply: 2)
512. RE digestion - (reply: 4)
513. HELP!!! - (reply: 3)
514. problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
515. Cloning of ITS region - (reply: 6)
516. preparation of LB/ampicillin/IPTG/X-gal plate - (reply: 3)
517. knockdown: yes; GFP: no ?! what is this? - (reply: 2)
518. SmaI blunt end ligation problem... - (reply: 3)
519. AAV construct? - (reply: 1)
520. No bands on agarose gel after restriction digestion. - (reply: 10)
521. inverse pcr - (reply: 2)
522. Viral cDNA Library - (reply: 3)
523. metylated cloning vector - restriction digest of the insert (reply: 2)
524. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
525. invitro mutagenesis using stragene kit - (reply: 4)
526. Secretion cloning vectors - (reply: 1)
527. SM10 lambda pir covers my plates - U$ 10.00 Amazon.com gift card for the most helpful feedback (reply: 4)
528. complementing a double knockout in E. coli - (reply: 1)
529. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
530. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
531. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
532. which G418 concentration and treatment period should be used? - (reply: 2)
533. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
534. TOPO TA Cloning of WGA products? - Is this possible? (reply: 2)
535. Chemical competent E.coli always from single colony ? - (reply: 2)
536. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
537. Strange results of cloning - insert always reverse-oriented (reply: 2)
538. electroporation - (reply: 2)
539. transfection or infection - difference? (reply: 1)
540. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
512. RE digestion - (reply: 4)
513. HELP!!! - (reply: 3)
514. problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
515. Cloning of ITS region - (reply: 6)
516. preparation of LB/ampicillin/IPTG/X-gal plate - (reply: 3)
517. knockdown: yes; GFP: no ?! what is this? - (reply: 2)
518. SmaI blunt end ligation problem... - (reply: 3)
519. AAV construct? - (reply: 1)
520. No bands on agarose gel after restriction digestion. - (reply: 10)
521. inverse pcr - (reply: 2)
522. Viral cDNA Library - (reply: 3)
523. metylated cloning vector - restriction digest of the insert (reply: 2)
524. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
525. invitro mutagenesis using stragene kit - (reply: 4)
526. Secretion cloning vectors - (reply: 1)
527. SM10 lambda pir covers my plates - U$ 10.00 Amazon.com gift card for the most helpful feedback (reply: 4)
528. complementing a double knockout in E. coli - (reply: 1)
529. dephosphorilation of vector ends - could SAP alter sticky ends? (reply: 3)
530. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
531. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
532. which G418 concentration and treatment period should be used? - (reply: 2)
533. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
534. TOPO TA Cloning of WGA products? - Is this possible? (reply: 2)
535. Chemical competent E.coli always from single colony ? - (reply: 2)
536. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
537. Strange results of cloning - insert always reverse-oriented (reply: 2)
538. electroporation - (reply: 2)
539. transfection or infection - difference? (reply: 1)
540. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)