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Top : New Forum Archives (2009-): : Molecular-Cloning
511. mamallian expression vector that has FLAG tag and GFP - (reply: 3)
512. Ligation into a BAC? - (reply: 2)
513. oligo continually appears less on gel compared to other same load - (reply: 2)
514. Is it possible to make your own Rosetta(DE3) pLysS by transforming pLysS-Rosetta - (reply: 1)
515. plasmid tetR and tet promoter - (reply: 2)
516. unable to amplify an infectious retrovirus clone - (reply: 4)
517. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
518. No bands at all after double digestion - (reply: 1)
519. Digest for insert orientation - (reply: 4)
520. Colonies from transformation plate are not growing...:( - (reply: 5)
521. Problem with ligating a small insert (LoxP site) - (reply: 5)
522. Is the genomic DNA similar among different cell lines? - (reply: 4)
523. molecular cloning - (reply: 3)
524. Swich selection in plasmid - (reply: 3)
525. Sub-cloning and transformation - (reply: 17)
526. cloning without phosphorylation - (reply: 7)
527. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
528. A good vector to make gene library? - (reply: 5)
529. problems with midiprep - (reply: 3)
530. Seeking pECFP.N1 and pET 42 Vectors - (reply: 3)
531. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
532. 900bp insertion into PIRESnEO,problem - (reply: 2)
533. fusion of cDNA expressing protein with gfp - (reply: 1)
534. Did my EcoRI restriction digestion work? picture included! - (reply: 49)
535. Problem with plasmids transformation - (reply: 6)
536. Cloning genetic sequences of proteins. - (reply: 1)
537. problem of restriction enzyme digestion - (reply: 3)
538. Site-directed, PCR works but no colonies - (reply: 2)
539. Simoltanious Multi-restriction digestion - (reply: 3)
540. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)