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Top : New Forum Archives (2009-): : Molecular-Cloning
511. Co-transfection with 2 plasmids - (reply: 10)
512. Low passage HEK293?? - (reply: 3)
513. Vector Digestion Problem - (reply: 4)
514. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
515. Transformation with a big plasmid - (reply: 4)
516. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
517. Three way ligation problem - (reply: 6)
518. problems with recovery of dna from gel and ligation - (reply: 5)
519. Colony PCR and digestion with KpnI - (reply: 10)
520. Greenish tinge to bacterial culture - (reply: 3)
521. SURE E. coli competent cells - (reply: 1)
522. Ligation - (reply: 20)
523. E. coli growing on plates and bot not in LB medium - (reply: 10)
524. *** Human Insulin promoter sequence*** - (reply: 2)
525. digested plasmid - (reply: 2)
526. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
527. Subcloning - RE digest and ligation and transformation - (reply: 5)
528. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
529. Making DNA Linkers for cloning purposes - (reply: 1)
530. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
531. Dam-/+ e. coli strains - (reply: 6)
532. cloning short genomic DNA fragments - (reply: 1)
533. finding the protein and nucleotide matches - (reply: 1)
534. DH10B with big construct question - (reply: 2)
535. QIAGEN Spin Columns and Minipreps - Alternatives?! - (reply: 15)
536. cloning to pET28a - (reply: 1)
537. Weird Library Transformation Results - (reply: 10)
538. Injecting mice with lentivirus - (reply: 1)
539. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
540. Alkaline phosphate treatment of vector SalI to ligate it to insert SalI site - (reply: 8)