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Top : New Forum Archives (2009-): : Molecular-Cloning
511. CIAP dilution - (reply: 5)
512. Problems in RNAi construction by using pUC19 - (reply: 3)
513. Unable to clone in E. coli cells - (reply: 3)
514. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
515. plasmid rearrangement or sabotage - (reply: 2)
516. pLDR20 Vector - (reply: 1)
517. surprising behavior of transformants needs immediate advice! - (reply: 8)
518. cloning antisense promoter in pGL3Basic - (reply: 2)
519. PhiX174 DNA - (reply: 1)
520. Competent bacterial cells - (reply: 2)
521. Replacing puramycin in a pLKO1.puro - (reply: 1)
522. colony cracking for screening of positive clones - (reply: 2)
523. deletion of large fregments - (reply: 3)
524. Linearising plasmid before transfection? - (reply: 2)
525. Smear in plasmid sample - (reply: 9)
526. about a book - (reply: 1)
527. Cloning Problem! I need help - (reply: 3)
528. Help designing primers for use in In-fushion cloning - (reply: 4)
529. Repeat of desired mutation in cloning product - (reply: 1)
530. vector dephosphorylation - (reply: 5)
531. ura3 mutant yeast strain grows on SD-uracil- - (reply: 1)
532. DH10BAC - (reply: 3)
533. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
534. protein is not expessing in HEK 293 cells - (reply: 2)
535. Restriction problems - (reply: 4)
536. cloning a gene in to a plasmid - (reply: 5)
537. no product on gel after restriction digestion - (reply: 3)
538. Gateway technology. - (reply: 4)
539. Primer concentration for cDNA synthesis using GSP - (reply: 1)
540. cloning from cDNA but got a much shorter PCR product - (reply: 4)