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Top : New Forum Archives (2009-): : Molecular-Cloning
511. Agrobacterium LBA4404 transformation - (reply: 1)
512. TOPO TA Cloning Troubleshooting - (reply: 1)
513. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
514. Tricistronic vector - (reply: 1)
515. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
516. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
517. Directions of genes expression illustrations - (reply: 5)
518. Plasmid DNA at RT for a few days - (reply: 4)
519. TOPO TA Cloning - (reply: 1)
520. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
521. No insert in subcloning - (reply: 3)
522. Digest does not confirm presence of fragment - (reply: 5)
523. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
524. pGL3 Promoter replacing TSS bases - (reply: 1)
525. lipofectamine 2000:DNA complex stability - (reply: 2)
526. Problems with Ecoli glycerol stock - (reply: 6)
527. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
528. CIAP dilution - (reply: 5)
529. Problems in RNAi construction by using pUC19 - (reply: 3)
530. Unable to clone in E. coli cells - (reply: 3)
531. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
532. plasmid rearrangement or sabotage - (reply: 2)
533. pLDR20 Vector - (reply: 1)
534. surprising behavior of transformants needs immediate advice! - (reply: 8)
535. cloning antisense promoter in pGL3Basic - (reply: 2)
536. PhiX174 DNA - (reply: 1)
537. Competent bacterial cells - (reply: 2)
538. Replacing puramycin in a pLKO1.puro - (reply: 1)
539. colony cracking for screening of positive clones - (reply: 2)
540. deletion of large fregments - (reply: 3)