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Top : New Forum Archives (2009-): : Molecular-Cloning
511. Large quantity heat shock trouble? - (reply: 3)
512. molecular cloning - (reply: 7)
513. Cannot see insert with colony pcr - (reply: 6)
514. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
515. help with a vector - (reply: 3)
516. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
517. Another cloning hell....no transformants - (reply: 1)
518. Agrobacterium LBA4404 transformation - (reply: 1)
519. TOPO TA Cloning Troubleshooting - (reply: 1)
520. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
521. Tricistronic vector - (reply: 1)
522. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
523. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
524. Directions of genes expression illustrations - (reply: 5)
525. Plasmid DNA at RT for a few days - (reply: 4)
526. TOPO TA Cloning - (reply: 1)
527. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
528. No insert in subcloning - (reply: 3)
529. Digest does not confirm presence of fragment - (reply: 5)
530. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
531. pGL3 Promoter replacing TSS bases - (reply: 1)
532. lipofectamine 2000:DNA complex stability - (reply: 2)
533. Problems with Ecoli glycerol stock - (reply: 6)
534. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
535. CIAP dilution - (reply: 5)
536. Problems in RNAi construction by using pUC19 - (reply: 3)
537. Unable to clone in E. coli cells - (reply: 3)
538. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
539. plasmid rearrangement or sabotage - (reply: 2)
540. pLDR20 Vector - (reply: 1)