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Top : New Forum Archives (2009-): : Molecular-Cloning
511. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
512. help with a vector - (reply: 3)
513. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
514. Another cloning hell....no transformants - (reply: 1)
515. Agrobacterium LBA4404 transformation - (reply: 1)
516. TOPO TA Cloning Troubleshooting - (reply: 1)
517. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
518. Tricistronic vector - (reply: 1)
519. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
520. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
521. Directions of genes expression illustrations - (reply: 5)
522. Plasmid DNA at RT for a few days - (reply: 4)
523. TOPO TA Cloning - (reply: 1)
524. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
525. No insert in subcloning - (reply: 3)
526. Digest does not confirm presence of fragment - (reply: 5)
527. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
528. pGL3 Promoter replacing TSS bases - (reply: 1)
529. lipofectamine 2000:DNA complex stability - (reply: 2)
530. Problems with Ecoli glycerol stock - (reply: 6)
531. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
532. CIAP dilution - (reply: 5)
533. Problems in RNAi construction by using pUC19 - (reply: 3)
534. Unable to clone in E. coli cells - (reply: 3)
535. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
536. plasmid rearrangement or sabotage - (reply: 2)
537. pLDR20 Vector - (reply: 1)
538. surprising behavior of transformants needs immediate advice! - (reply: 8)
539. cloning antisense promoter in pGL3Basic - (reply: 2)
540. PhiX174 DNA - (reply: 1)