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Top : New Forum Archives (2009-): : Molecular-Cloning
511. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
512. No insert in subcloning - (reply: 3)
513. Digest does not confirm presence of fragment - (reply: 5)
514. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
515. pGL3 Promoter replacing TSS bases - (reply: 1)
516. lipofectamine 2000:DNA complex stability - (reply: 2)
517. Problems with Ecoli glycerol stock - (reply: 6)
518. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
519. CIAP dilution - (reply: 5)
520. Problems in RNAi construction by using pUC19 - (reply: 3)
521. Unable to clone in E. coli cells - (reply: 3)
522. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
523. plasmid rearrangement or sabotage - (reply: 2)
524. pLDR20 Vector - (reply: 1)
525. surprising behavior of transformants needs immediate advice! - (reply: 8)
526. cloning antisense promoter in pGL3Basic - (reply: 2)
527. PhiX174 DNA - (reply: 1)
528. Competent bacterial cells - (reply: 2)
529. Replacing puramycin in a pLKO1.puro - (reply: 1)
530. colony cracking for screening of positive clones - (reply: 2)
531. deletion of large fregments - (reply: 3)
532. Linearising plasmid before transfection? - (reply: 2)
533. Smear in plasmid sample - (reply: 9)
534. about a book - (reply: 1)
535. Cloning Problem! I need help - (reply: 3)
536. Help designing primers for use in In-fushion cloning - (reply: 4)
537. Repeat of desired mutation in cloning product - (reply: 1)
538. vector dephosphorylation - (reply: 5)
539. ura3 mutant yeast strain grows on SD-uracil- - (reply: 1)
540. DH10BAC - (reply: 3)