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Top : New Forum Archives (2009-): : Molecular-Cloning
511. Simoltanious Multi-restriction digestion - (reply: 3)
512. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
513. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
514. Restriction digestion problems - (reply: 3)
515. should I do blunt end my pcr product before ligation - (reply: 2)
516. Maxiprep of freeze/thawed pellet. - (reply: 1)
517. Digest with AarI and Pml I - (reply: 1)
518. Help with CGL gene cloning problems - (reply: 1)
519. screening for GPF - (reply: 10)
520. Creating a pGEM-T easy + insert sequence map - (reply: 1)
521. where to get vector overexpressing caveolin-1? - (reply: 2)
522. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
523. pINDUCER series - (reply: 1)
524. LZRS vector details - (reply: 1)
525. 12.4kb vector 6.7kb insert - (reply: 2)
526. Stable transfection - (reply: 2)
527. Using normal competent cells in electroporation method? - (reply: 1)
528. an effective way to do a yeast colony pcr - (reply: 2)
529. pGEM-T Easy vector contamination - (reply: 2)
530. recover old transformed DH5 - (reply: 1)
531. NZY+Broth can be replaced by LB Broth in mutagenesis exp - (reply: 2)
532. oligo(dt) 15 vs random primers - (reply: 3)
533. I need bynary vector pART27 - (reply: 1)
534. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
535. Why don't I get the expected entry clones when I use the gateway system?? - (reply: 2)
536. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
537. cloning into Pgem t easy - (reply: 1)
538. How to get pure protein of a gene - (reply: 3)
539. How to insert genes into chromosome of E. coli - (reply: 1)
540. Gene synthesis - (reply: 2)