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Top : New Forum Archives (2009-): : Molecular-Cloning
511. Colonies from transformation plate are not growing...:( - (reply: 5)
512. Problem with ligating a small insert (LoxP site) - (reply: 5)
513. Is the genomic DNA similar among different cell lines? - (reply: 4)
514. molecular cloning - (reply: 3)
515. Swich selection in plasmid - (reply: 3)
516. Sub-cloning and transformation - (reply: 17)
517. cloning without phosphorylation - (reply: 7)
518. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
519. A good vector to make gene library? - (reply: 5)
520. problems with midiprep - (reply: 3)
521. Seeking pECFP.N1 and pET 42 Vectors - (reply: 3)
522. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
523. 900bp insertion into PIRESnEO,problem - (reply: 2)
524. fusion of cDNA expressing protein with gfp - (reply: 1)
525. Did my EcoRI restriction digestion work? picture included! - (reply: 49)
526. Problem with plasmids transformation - (reply: 6)
527. Cloning genetic sequences of proteins. - (reply: 1)
528. problem of restriction enzyme digestion - (reply: 3)
529. Site-directed, PCR works but no colonies - (reply: 2)
530. Simoltanious Multi-restriction digestion - (reply: 3)
531. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
532. can you recommend a gateway mammalian expression vector? Please? :) - (reply: 1)
533. Restriction digestion problems - (reply: 3)
534. should I do blunt end my pcr product before ligation - (reply: 2)
535. Maxiprep of freeze/thawed pellet. - (reply: 1)
536. Digest with AarI and Pml I - (reply: 1)
537. Help with CGL gene cloning problems - (reply: 1)
538. screening for GPF - (reply: 10)
539. Creating a pGEM-T easy + insert sequence map - (reply: 1)
540. where to get vector overexpressing caveolin-1? - (reply: 2)