Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
511. an effective way to do a yeast colony pcr - (reply: 2)
512. pGEM-T Easy vector contamination - (reply: 2)
513. recover old transformed DH5 - (reply: 1)
514. NZY+Broth can be replaced by LB Broth in mutagenesis exp - (reply: 2)
515. oligo(dt) 15 vs random primers - (reply: 3)
516. I need bynary vector pART27 - (reply: 1)
517. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
518. Why don't I get the expected entry clones when I use the gateway system?? - (reply: 2)
519. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
520. cloning into Pgem t easy - (reply: 1)
521. How to get pure protein of a gene - (reply: 3)
522. How to insert genes into chromosome of E. coli - (reply: 1)
523. Gene synthesis - (reply: 2)
524. I canīt obtain the amount of DNA required for ligation of blunt ends - (reply: 2)
525. Pls help me with this plasmid!!! - (reply: 1)
526. Problems with Restriction Digest Results - (reply: 7)
527. Methods of plasmid transformation? only heat shock? - (reply: 1)
528. Digestion - (reply: 2)
529. Digestion of Genomic DNA - (reply: 1)
530. Isolation of a Gene of Interest for Subcloning - (reply: 8)
531. E.coli grow on neg. control - (reply: 1)
532. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
533. Need help troubleshooting digest. - (reply: 1)
534. Competent cells question: JM101 vs DH5alpha - (reply: 2)
535. How to avoid plasmid instability - (reply: 4)
536. Question Help - (reply: 1)
537. Shear-induced damage of plasmid by vortexing - (reply: 1)
538. Problems with blunt end ligation - (reply: 3)
539. Problem with Transforming E. coli DH10Bac - (reply: 8)
540. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)