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Top : New Forum Archives (2009-): : Molecular-Cloning
511. lipofectamine 2000:DNA complex stability - (reply: 2)
512. Problems with Ecoli glycerol stock - (reply: 6)
513. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
514. CIAP dilution - (reply: 5)
515. Problems in RNAi construction by using pUC19 - (reply: 3)
516. Unable to clone in E. coli cells - (reply: 3)
517. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
518. plasmid rearrangement or sabotage - (reply: 2)
519. pLDR20 Vector - (reply: 1)
520. surprising behavior of transformants needs immediate advice! - (reply: 8)
521. cloning antisense promoter in pGL3Basic - (reply: 2)
522. PhiX174 DNA - (reply: 1)
523. Competent bacterial cells - (reply: 2)
524. Replacing puramycin in a pLKO1.puro - (reply: 1)
525. colony cracking for screening of positive clones - (reply: 2)
526. deletion of large fregments - (reply: 3)
527. Linearising plasmid before transfection? - (reply: 2)
528. Smear in plasmid sample - (reply: 9)
529. about a book - (reply: 1)
530. Cloning Problem! I need help - (reply: 3)
531. Help designing primers for use in In-fushion cloning - (reply: 4)
532. Repeat of desired mutation in cloning product - (reply: 1)
533. vector dephosphorylation - (reply: 5)
534. ura3 mutant yeast strain grows on SD-uracil- - (reply: 1)
535. DH10BAC - (reply: 3)
536. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
537. protein is not expessing in HEK 293 cells - (reply: 2)
538. Restriction problems - (reply: 4)
539. cloning a gene in to a plasmid - (reply: 5)
540. no product on gel after restriction digestion - (reply: 3)