Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
511. Difference between types of bacterial culture broths? - (reply: 4)
512. Co-transfection with 2 plasmids - (reply: 10)
513. Low passage HEK293?? - (reply: 3)
514. Vector Digestion Problem - (reply: 4)
515. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
516. Transformation with a big plasmid - (reply: 4)
517. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
518. Three way ligation problem - (reply: 6)
519. problems with recovery of dna from gel and ligation - (reply: 5)
520. Colony PCR and digestion with KpnI - (reply: 10)
521. Greenish tinge to bacterial culture - (reply: 3)
522. SURE E. coli competent cells - (reply: 1)
523. Ligation - (reply: 20)
524. E. coli growing on plates and bot not in LB medium - (reply: 10)
525. *** Human Insulin promoter sequence*** - (reply: 2)
526. digested plasmid - (reply: 2)
527. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
528. Subcloning - RE digest and ligation and transformation - (reply: 5)
529. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
530. Making DNA Linkers for cloning purposes - (reply: 1)
531. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
532. Dam-/+ e. coli strains - (reply: 6)
533. cloning short genomic DNA fragments - (reply: 1)
534. finding the protein and nucleotide matches - (reply: 1)
535. DH10B with big construct question - (reply: 2)
536. QIAGEN Spin Columns and Minipreps - Alternatives?! - (reply: 15)
537. cloning to pET28a - (reply: 1)
538. Weird Library Transformation Results - (reply: 10)
539. Injecting mice with lentivirus - (reply: 1)
540. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)