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Top : New Forum Archives (2009-): : Molecular-Cloning
511. Cannot see insert with colony pcr - (reply: 6)
512. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
513. help with a vector - (reply: 3)
514. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
515. Another cloning hell....no transformants - (reply: 1)
516. Agrobacterium LBA4404 transformation - (reply: 1)
517. TOPO TA Cloning Troubleshooting - (reply: 1)
518. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
519. Tricistronic vector - (reply: 1)
520. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
521. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
522. Directions of genes expression illustrations - (reply: 5)
523. Plasmid DNA at RT for a few days - (reply: 4)
524. TOPO TA Cloning - (reply: 1)
525. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
526. No insert in subcloning - (reply: 3)
527. Digest does not confirm presence of fragment - (reply: 5)
528. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
529. pGL3 Promoter replacing TSS bases - (reply: 1)
530. lipofectamine 2000:DNA complex stability - (reply: 2)
531. Problems with Ecoli glycerol stock - (reply: 6)
532. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
533. CIAP dilution - (reply: 5)
534. Problems in RNAi construction by using pUC19 - (reply: 3)
535. Unable to clone in E. coli cells - (reply: 3)
536. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
537. plasmid rearrangement or sabotage - (reply: 2)
538. pLDR20 Vector - (reply: 1)
539. surprising behavior of transformants needs immediate advice! - (reply: 8)
540. cloning antisense promoter in pGL3Basic - (reply: 2)