Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
391. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
392. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
393. How to select competent E. coli cells? - (reply: 7)
394. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
395. cDNA amplification problem - (reply: 4)
396. Ligation: two basic questions - (reply: 1)
397. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)
398. Unexpected base after deletion mutagenesis - (reply: 3)
399. Confirmation of ligation - (reply: 7)
400. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
401. Ligation of large ammount of insert (no transformation) - (reply: 7)
402. PCR and restriction enzyme digestion - (reply: 3)
403. Probelm with double digestion - (reply: 1)
404. Cloning not working, all steps checked, clueless - (reply: 1)
405. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
406. recombinant protein form - (reply: 2)
407. restricted PCR plasmid runs slower - (reply: 2)
408. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
409. High frequency of vector religation - (reply: 10)
410. Cloned but not expressing! - (reply: 8)
411. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
412. DNA ligation and trasformation - (reply: 6)
413. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
414. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
415. How to set up simultaneous digestion? - (reply: 3)
416. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
417. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
418. Freeze transformation mixture? - (reply: 2)
419. Cutting my plasmid made it larger - (reply: 7)
420. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)