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Top : New Forum Archives (2009-): : Molecular-Cloning
391. Cloning large fragments - (reply: 9)
392. Transformation colonies does not contain insert - (reply: 3)
393. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
394. Need urgent advise.. - (reply: 3)
395. Cloning Mitochondrial Targeting Sequence - (reply: 4)
396. problem in cloning - (reply: 2)
397. TA cloning: ligation problem or toxic construct? - (reply: 4)
398. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
399. Do I need to sequence after digestion? - (reply: 9)
400. quick change mutagenesis...... - (reply: 15)
401. Problem with double digestion: one enzyme is not working - (reply: 8)
402. Transient Transfection Controls (Gateway Technology) - (reply: 4)
403. Problems with crossover PCR - (reply: 2)
404. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
405. Two genes, same MCS - (reply: 1)
406. Including ATG in cloning? - (reply: 1)
407. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
408. Low copy vector for library generation - (reply: 2)
409. Can't find a recombinant after ligating - (reply: 4)
410. problems with plasmid miniprep - (reply: 8)
411. gene cloning - (reply: 1)
412. Is ligation really necessary? - (reply: 6)
413. problem with ligation of linker/adaptor - (reply: 2)
414. GFP -weak signal -why? - (reply: 1)
415. Ligation troubleshooting! Please help! - (reply: 5)
416. Truncated gene Cloning - (reply: 1)
417. Screening for ligation and transformation result - (reply: 2)
418. Problem with 3 way ligation - (reply: 2)
419. Query regarding primers for quick change mutagenesis - (reply: 3)
420. What is NEB's CutSmart Buffer? - (reply: 5)