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Top : New Forum Archives (2009-): : Molecular-Cloning
391. double transformation problem - (reply: 3)
392. Too many negative clones using Directional TOPO Cloning - (reply: 5)
393. competent cells - (reply: 1)
394. "Easiest" sticky end combination - (reply: 3)
395. Cloning advice - (reply: 3)
396. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
397. Cloning large fragments - (reply: 9)
398. Transformation colonies does not contain insert - (reply: 3)
399. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
400. Need urgent advise.. - (reply: 3)
401. Cloning Mitochondrial Targeting Sequence - (reply: 4)
402. problem in cloning - (reply: 2)
403. TA cloning: ligation problem or toxic construct? - (reply: 4)
404. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
405. Do I need to sequence after digestion? - (reply: 9)
406. quick change mutagenesis...... - (reply: 15)
407. Problem with double digestion: one enzyme is not working - (reply: 8)
408. Transient Transfection Controls (Gateway Technology) - (reply: 4)
409. Problems with crossover PCR - (reply: 2)
410. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
411. Two genes, same MCS - (reply: 1)
412. Including ATG in cloning? - (reply: 1)
413. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
414. Low copy vector for library generation - (reply: 2)
415. Can't find a recombinant after ligating - (reply: 4)
416. problems with plasmid miniprep - (reply: 8)
417. gene cloning - (reply: 1)
418. Is ligation really necessary? - (reply: 6)
419. problem with ligation of linker/adaptor - (reply: 2)
420. GFP -weak signal -why? - (reply: 1)