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Top : New Forum Archives (2009-): : Molecular-Cloning
391. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
392. Do I need to sequence after digestion? - (reply: 9)
393. quick change mutagenesis...... - (reply: 15)
394. Problem with double digestion: one enzyme is not working - (reply: 8)
395. Transient Transfection Controls (Gateway Technology) - (reply: 4)
396. Problems with crossover PCR - (reply: 2)
397. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
398. Two genes, same MCS - (reply: 1)
399. Including ATG in cloning? - (reply: 1)
400. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
401. Low copy vector for library generation - (reply: 2)
402. Can't find a recombinant after ligating - (reply: 4)
403. problems with plasmid miniprep - (reply: 8)
404. gene cloning - (reply: 1)
405. Is ligation really necessary? - (reply: 6)
406. problem with ligation of linker/adaptor - (reply: 2)
407. GFP -weak signal -why? - (reply: 1)
408. Ligation troubleshooting! Please help! - (reply: 5)
409. Truncated gene Cloning - (reply: 1)
410. Screening for ligation and transformation result - (reply: 2)
411. Problem with 3 way ligation - (reply: 2)
412. Query regarding primers for quick change mutagenesis - (reply: 3)
413. What is NEB's CutSmart Buffer? - (reply: 5)
414. Enzyme cutting: low efficiency - (reply: 5)
415. Issue with Xba1 and Dam methylation - (reply: 7)
416. Restriction site rearrangement - (reply: 1)
417. Mutation in cloning - (reply: 6)
418. Must the terminator be in frame? - (reply: 1)
419. sequencing problems - (reply: 2)
420. Understand the basics of molecular cloning - (reply: 6)