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Top : New Forum Archives (2009-): : Molecular-Cloning
391. Restriction Enzyme Digest not working - (reply: 2)
392. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 1)
393. Incomplete digestion of plasmid with single enzyme - (reply: 1)
394. problem with plasmid digestion - (reply: 5)
395. Difficult Ligation - Details Inside - (reply: 9)
396. pET 32 (+) - (reply: 1)
397. Alternative to pET Vectorsystem - (reply: 2)
398. pEGFP C-1/N-1 Cloning - (reply: 11)
399. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
400. Problems with growing out transformants in liquid LB - (reply: 3)
401. Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
402. Ethidiumbromide - (reply: 3)
403. TRYPSIN - (reply: 4)
404. Problem with cloning - what is wrong? - (reply: 6)
405. cloning: band at different site than desired after RE digestion - (reply: 11)
406. GST TAG - (reply: 3)
407. Residues on the N terminal GST Tag - (reply: 1)
408. Where to place the polyA signal? - (reply: 1)
409. identification of target sequence - (reply: 3)
410. cloning pcr insert into plasmid - (reply: 2)
411. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
412. Large quantity heat shock trouble? - (reply: 3)
413. molecular cloning - (reply: 7)
414. Cannot see insert with colony pcr - (reply: 6)
415. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
416. help with a vector - (reply: 3)
417. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
418. Another cloning hell....no transformants - (reply: 1)
419. Agrobacterium LBA4404 transformation - (reply: 1)
420. TOPO TA Cloning Troubleshooting - (reply: 1)