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Top : New Forum Archives (2009-): : Molecular-Cloning
391. Problem with 3 way ligation - (reply: 2)
392. Query regarding primers for quick change mutagenesis - (reply: 3)
393. What is NEB's CutSmart Buffer? - (reply: 5)
394. Enzyme cutting: low efficiency - (reply: 5)
395. Issue with Xba1 and Dam methylation - (reply: 7)
396. Restriction site rearrangement - (reply: 1)
397. Mutation in cloning - (reply: 6)
398. Must the terminator be in frame? - (reply: 1)
399. sequencing problems - (reply: 2)
400. Understand the basics of molecular cloning - (reply: 6)
401. Maintainence of transformed cells - (reply: 5)
402. Restriction digest band shift? - (reply: 1)
403. problem in cloning PCR primer design with restriction site - (reply: 4)
404. quick change mutagenesis...... - (reply: 1)
405. cloneJet (Fermentas) ligation buffer "stability" - (reply: 1)
406. Help for pGEM-T easy vector ligation problem - (reply: 2)
407. Co-transformation - (reply: 2)
408. Problems regarding point mutations.... - (reply: 4)
409. Question regarding yeast strain with ura3-52 mutation - (reply: 1)
410. pkp 59 vector - (reply: 2)
411. Vector insert ratio in cloning - (reply: 3)
412. polymerase to use for cloning - (reply: 4)
413. Oligo insert cloning - (reply: 3)
414. Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
415. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
416. How to select competent E. coli cells? - (reply: 7)
417. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
418. cDNA amplification problem - (reply: 4)
419. Ligation: two basic questions - (reply: 1)
420. Allelic Exchange in Pseudomonas aeruginosa - (reply: 1)