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Top : New Forum Archives (2009-): : Molecular-Cloning
391. competent cells - (reply: 1)
392. "Easiest" sticky end combination - (reply: 3)
393. Cloning advice - (reply: 3)
394. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
395. Cloning large fragments - (reply: 9)
396. Transformation colonies does not contain insert - (reply: 3)
397. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
398. Need urgent advise.. - (reply: 3)
399. Cloning Mitochondrial Targeting Sequence - (reply: 4)
400. problem in cloning - (reply: 2)
401. TA cloning: ligation problem or toxic construct? - (reply: 4)
402. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
403. Do I need to sequence after digestion? - (reply: 9)
404. quick change mutagenesis...... - (reply: 15)
405. Problem with double digestion: one enzyme is not working - (reply: 8)
406. Transient Transfection Controls (Gateway Technology) - (reply: 4)
407. Problems with crossover PCR - (reply: 2)
408. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
409. Two genes, same MCS - (reply: 1)
410. Including ATG in cloning? - (reply: 1)
411. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
412. Low copy vector for library generation - (reply: 2)
413. Can't find a recombinant after ligating - (reply: 4)
414. problems with plasmid miniprep - (reply: 8)
415. gene cloning - (reply: 1)
416. Is ligation really necessary? - (reply: 6)
417. problem with ligation of linker/adaptor - (reply: 2)
418. GFP -weak signal -why? - (reply: 1)
419. Ligation troubleshooting! Please help! - (reply: 5)
420. Truncated gene Cloning - (reply: 1)