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Top : New Forum Archives (2009-): : Molecular-Cloning
391. Problems with Digestion? - (reply: 7)
392. plasmid digestion - problem with enzymes? - (reply: 4)
393. Problem with cloning - (reply: 7)
394. double transformation problem - (reply: 3)
395. Too many negative clones using Directional TOPO Cloning - (reply: 5)
396. competent cells - (reply: 1)
397. "Easiest" sticky end combination - (reply: 3)
398. Cloning advice - (reply: 3)
399. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
400. Cloning large fragments - (reply: 9)
401. Transformation colonies does not contain insert - (reply: 3)
402. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
403. Need urgent advise.. - (reply: 3)
404. Cloning Mitochondrial Targeting Sequence - (reply: 4)
405. problem in cloning - (reply: 2)
406. TA cloning: ligation problem or toxic construct? - (reply: 4)
407. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
408. Do I need to sequence after digestion? - (reply: 9)
409. quick change mutagenesis...... - (reply: 15)
410. Problem with double digestion: one enzyme is not working - (reply: 8)
411. Transient Transfection Controls (Gateway Technology) - (reply: 4)
412. Problems with crossover PCR - (reply: 2)
413. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
414. Two genes, same MCS - (reply: 1)
415. Including ATG in cloning? - (reply: 1)
416. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
417. Low copy vector for library generation - (reply: 2)
418. Can't find a recombinant after ligating - (reply: 4)
419. problems with plasmid miniprep - (reply: 8)
420. gene cloning - (reply: 1)