Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
391. Where to place the polyA signal? - (reply: 1)
392. identification of target sequence - (reply: 3)
393. cloning pcr insert into plasmid - (reply: 2)
394. TOPO TA CLONING - MOLAR RATIO - (reply: 2)
395. Large quantity heat shock trouble? - (reply: 3)
396. molecular cloning - (reply: 7)
397. Cannot see insert with colony pcr - (reply: 6)
398. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
399. help with a vector - (reply: 3)
400. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
401. Another cloning hell....no transformants - (reply: 1)
402. Agrobacterium LBA4404 transformation - (reply: 1)
403. TOPO TA Cloning Troubleshooting - (reply: 1)
404. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
405. Tricistronic vector - (reply: 1)
406. gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
407. How would you find a certain tissue specific promoter for my construct? - (reply: 2)
408. Directions of genes expression illustrations - (reply: 5)
409. Plasmid DNA at RT for a few days - (reply: 4)
410. TOPO TA Cloning - (reply: 1)
411. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
412. No insert in subcloning - (reply: 3)
413. Digest does not confirm presence of fragment - (reply: 5)
414. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
415. pGL3 Promoter replacing TSS bases - (reply: 1)
416. lipofectamine 2000:DNA complex stability - (reply: 2)
417. Problems with Ecoli glycerol stock - (reply: 6)
418. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
419. CIAP dilution - (reply: 5)
420. Problems in RNAi construction by using pUC19 - (reply: 3)