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Top : New Forum Archives (2009-): : Molecular-Cloning
391. plasmid digestion - problem with enzymes? - (reply: 4)
392. Problem with cloning - (reply: 7)
393. double transformation problem - (reply: 3)
394. Too many negative clones using Directional TOPO Cloning - (reply: 5)
395. competent cells - (reply: 1)
396. "Easiest" sticky end combination - (reply: 3)
397. Cloning advice - (reply: 3)
398. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
399. Cloning large fragments - (reply: 9)
400. Transformation colonies does not contain insert - (reply: 3)
401. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
402. Need urgent advise.. - (reply: 3)
403. Cloning Mitochondrial Targeting Sequence - (reply: 4)
404. problem in cloning - (reply: 2)
405. TA cloning: ligation problem or toxic construct? - (reply: 4)
406. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
407. Do I need to sequence after digestion? - (reply: 9)
408. quick change mutagenesis...... - (reply: 15)
409. Problem with double digestion: one enzyme is not working - (reply: 8)
410. Transient Transfection Controls (Gateway Technology) - (reply: 4)
411. Problems with crossover PCR - (reply: 2)
412. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
413. Two genes, same MCS - (reply: 1)
414. Including ATG in cloning? - (reply: 1)
415. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
416. Low copy vector for library generation - (reply: 2)
417. Can't find a recombinant after ligating - (reply: 4)
418. problems with plasmid miniprep - (reply: 8)
419. gene cloning - (reply: 1)
420. Is ligation really necessary? - (reply: 6)