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Top : New Forum Archives (2009-): : Molecular-Cloning
391. "Easiest" sticky end combination - (reply: 3)
392. Cloning advice - (reply: 3)
393. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
394. Cloning large fragments - (reply: 9)
395. Transformation colonies does not contain insert - (reply: 3)
396. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
397. Need urgent advise.. - (reply: 3)
398. Cloning Mitochondrial Targeting Sequence - (reply: 4)
399. problem in cloning - (reply: 2)
400. TA cloning: ligation problem or toxic construct? - (reply: 4)
401. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
402. Do I need to sequence after digestion? - (reply: 9)
403. quick change mutagenesis...... - (reply: 15)
404. Problem with double digestion: one enzyme is not working - (reply: 8)
405. Transient Transfection Controls (Gateway Technology) - (reply: 4)
406. Problems with crossover PCR - (reply: 2)
407. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
408. Two genes, same MCS - (reply: 1)
409. Including ATG in cloning? - (reply: 1)
410. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
411. Low copy vector for library generation - (reply: 2)
412. Can't find a recombinant after ligating - (reply: 4)
413. problems with plasmid miniprep - (reply: 8)
414. gene cloning - (reply: 1)
415. Is ligation really necessary? - (reply: 6)
416. problem with ligation of linker/adaptor - (reply: 2)
417. GFP -weak signal -why? - (reply: 1)
418. Ligation troubleshooting! Please help! - (reply: 5)
419. Truncated gene Cloning - (reply: 1)
420. Screening for ligation and transformation result - (reply: 2)