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391. Transforming bacteria with plasmid from filter paper, months later - (reply: 5)
392. XhoI digestion - (reply: 12)
393. Lenti vector ligation - (reply: 2)
394. Cloning from a pGEMT Easy vector - (reply: 3)
395. Distance between RE sites for double digestion - (reply: 5)
396. Plasmid compatible with pBAD24 - (reply: 1)
397. I cant get colony - (reply: 3)
398. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
399. Vector with inducible/repressible system - (reply: 1)
400. cloning problem - (reply: 9)
401. Cloning genes into plant vector - (reply: 1)
402. Do amino acids and his tag on vector affect protein of interest? - (reply: 4)
403. Three-way ligation in two steps? - (reply: 3)
404. How does circular, uncut pGEM-T migrate? - (reply: 2)
405. Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)
406. 3-way ligation of 2 annealed dsDNAs and pGEM-T Easy - (reply: 6)
407. Cloning App Released from Life Technologies - (reply: 2)
408. gateway cloning: is BP-cloning "reversible" by LR-reaction? - (reply: 2)
409. Dissolve IPTG in EtOH - (reply: 3)
410. problem with transient transfection - (reply: 6)
411. Recombinant construct looks smaller than empty vector - (reply: 3)
412. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
413. hygromycin concentration for e-coli selection - (reply: 1)
414. orientation of promoter into viral construct - (reply: 1)
415. problems with PCR confirmation of insert . HELP ! - (reply: 1)
416. "CpG methylation: Blocked by some combinations of overlapping" - (reply: 1)
417. Loss of promoter during ligation - (reply: 2)
418. Enzyme restriction - (reply: 8)
419. Colony PCR Problem!!!! - (reply: 5)
420. Removing unwanted sequence from pUC19 - (reply: 4)
392. XhoI digestion - (reply: 12)
393. Lenti vector ligation - (reply: 2)
394. Cloning from a pGEMT Easy vector - (reply: 3)
395. Distance between RE sites for double digestion - (reply: 5)
396. Plasmid compatible with pBAD24 - (reply: 1)
397. I cant get colony - (reply: 3)
398. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
399. Vector with inducible/repressible system - (reply: 1)
400. cloning problem - (reply: 9)
401. Cloning genes into plant vector - (reply: 1)
402. Do amino acids and his tag on vector affect protein of interest? - (reply: 4)
403. Three-way ligation in two steps? - (reply: 3)
404. How does circular, uncut pGEM-T migrate? - (reply: 2)
405. Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)
406. 3-way ligation of 2 annealed dsDNAs and pGEM-T Easy - (reply: 6)
407. Cloning App Released from Life Technologies - (reply: 2)
408. gateway cloning: is BP-cloning "reversible" by LR-reaction? - (reply: 2)
409. Dissolve IPTG in EtOH - (reply: 3)
410. problem with transient transfection - (reply: 6)
411. Recombinant construct looks smaller than empty vector - (reply: 3)
412. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
413. hygromycin concentration for e-coli selection - (reply: 1)
414. orientation of promoter into viral construct - (reply: 1)
415. problems with PCR confirmation of insert . HELP ! - (reply: 1)
416. "CpG methylation: Blocked by some combinations of overlapping" - (reply: 1)
417. Loss of promoter during ligation - (reply: 2)
418. Enzyme restriction - (reply: 8)
419. Colony PCR Problem!!!! - (reply: 5)
420. Removing unwanted sequence from pUC19 - (reply: 4)