Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
391. Few colonies frm sticky end cloning - (reply: 3)
392. transformation problem - (reply: 8)
393. Problems with Digestion? - (reply: 7)
394. plasmid digestion - problem with enzymes? - (reply: 4)
395. Problem with cloning - (reply: 7)
396. double transformation problem - (reply: 3)
397. Too many negative clones using Directional TOPO Cloning - (reply: 5)
398. competent cells - (reply: 1)
399. "Easiest" sticky end combination - (reply: 3)
400. Cloning advice - (reply: 3)
401. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
402. Cloning large fragments - (reply: 9)
403. Transformation colonies does not contain insert - (reply: 3)
404. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
405. Need urgent advise.. - (reply: 3)
406. Cloning Mitochondrial Targeting Sequence - (reply: 4)
407. problem in cloning - (reply: 2)
408. TA cloning: ligation problem or toxic construct? - (reply: 4)
409. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
410. Do I need to sequence after digestion? - (reply: 9)
411. quick change mutagenesis...... - (reply: 15)
412. Problem with double digestion: one enzyme is not working - (reply: 8)
413. Transient Transfection Controls (Gateway Technology) - (reply: 4)
414. Problems with crossover PCR - (reply: 2)
415. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
416. Two genes, same MCS - (reply: 1)
417. Including ATG in cloning? - (reply: 1)
418. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
419. Low copy vector for library generation - (reply: 2)
420. Can't find a recombinant after ligating - (reply: 4)