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Top : New Forum Archives (2009-): : Molecular-Cloning
391. transformation problem - (reply: 8)
392. Problems with Digestion? - (reply: 7)
393. plasmid digestion - problem with enzymes? - (reply: 4)
394. Problem with cloning - (reply: 7)
395. double transformation problem - (reply: 3)
396. Too many negative clones using Directional TOPO Cloning - (reply: 5)
397. competent cells - (reply: 1)
398. "Easiest" sticky end combination - (reply: 3)
399. Cloning advice - (reply: 3)
400. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
401. Cloning large fragments - (reply: 9)
402. Transformation colonies does not contain insert - (reply: 3)
403. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
404. Need urgent advise.. - (reply: 3)
405. Cloning Mitochondrial Targeting Sequence - (reply: 4)
406. problem in cloning - (reply: 2)
407. TA cloning: ligation problem or toxic construct? - (reply: 4)
408. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
409. Do I need to sequence after digestion? - (reply: 9)
410. quick change mutagenesis...... - (reply: 15)
411. Problem with double digestion: one enzyme is not working - (reply: 8)
412. Transient Transfection Controls (Gateway Technology) - (reply: 4)
413. Problems with crossover PCR - (reply: 2)
414. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
415. Two genes, same MCS - (reply: 1)
416. Including ATG in cloning? - (reply: 1)
417. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
418. Low copy vector for library generation - (reply: 2)
419. Can't find a recombinant after ligating - (reply: 4)
420. problems with plasmid miniprep - (reply: 8)