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Top : New Forum Archives (2009-): : Molecular-Cloning
391. Problem with double digestion: one enzyme is not working - (reply: 8)
392. Transient Transfection Controls (Gateway Technology) - (reply: 4)
393. Problems with crossover PCR - (reply: 2)
394. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
395. Two genes, same MCS - (reply: 1)
396. Including ATG in cloning? - (reply: 1)
397. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
398. Low copy vector for library generation - (reply: 2)
399. Can't find a recombinant after ligating - (reply: 4)
400. problems with plasmid miniprep - (reply: 8)
401. gene cloning - (reply: 1)
402. Is ligation really necessary? - (reply: 6)
403. problem with ligation of linker/adaptor - (reply: 2)
404. GFP -weak signal -why? - (reply: 1)
405. Ligation troubleshooting! Please help! - (reply: 5)
406. Truncated gene Cloning - (reply: 1)
407. Screening for ligation and transformation result - (reply: 2)
408. Problem with 3 way ligation - (reply: 2)
409. Query regarding primers for quick change mutagenesis - (reply: 3)
410. What is NEB's CutSmart Buffer? - (reply: 5)
411. Enzyme cutting: low efficiency - (reply: 5)
412. Issue with Xba1 and Dam methylation - (reply: 7)
413. Restriction site rearrangement - (reply: 1)
414. Mutation in cloning - (reply: 6)
415. Must the terminator be in frame? - (reply: 1)
416. sequencing problems - (reply: 2)
417. Understand the basics of molecular cloning - (reply: 6)
418. Maintainence of transformed cells - (reply: 5)
419. Restriction digest band shift? - (reply: 1)
420. problem in cloning PCR primer design with restriction site - (reply: 4)