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Top : New Forum Archives (2009-): : Molecular-Cloning
391. cloning from cDNA but got a much shorter PCR product - (reply: 4)
392. GeneArt vs Quikchange - (reply: 1)
393. why do some colonies keep growing in the chiller? - (reply: 7)
394. Cloning & Expression Problem in HEK-293Ts - (reply: 6)
395. Difference between types of bacterial culture broths? - (reply: 4)
396. Co-transfection with 2 plasmids - (reply: 10)
397. Low passage HEK293?? - (reply: 3)
398. Vector Digestion Problem - (reply: 4)
399. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
400. Transformation with a big plasmid - (reply: 4)
401. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
402. Three way ligation problem - (reply: 6)
403. problems with recovery of dna from gel and ligation - (reply: 5)
404. Colony PCR and digestion with KpnI - (reply: 10)
405. Greenish tinge to bacterial culture - (reply: 3)
406. SURE E. coli competent cells - (reply: 1)
407. Ligation - (reply: 20)
408. E. coli growing on plates and bot not in LB medium - (reply: 10)
409. *** Human Insulin promoter sequence*** - (reply: 2)
410. digested plasmid - (reply: 2)
411. can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
412. Subcloning - RE digest and ligation and transformation - (reply: 5)
413. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
414. Making DNA Linkers for cloning purposes - (reply: 1)
415. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
416. Dam-/+ e. coli strains - (reply: 6)
417. cloning short genomic DNA fragments - (reply: 1)
418. finding the protein and nucleotide matches - (reply: 1)
419. DH10B with big construct question - (reply: 2)
420. QIAGEN Spin Columns and Minipreps - Alternatives?! - (reply: 14)