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Top : New Forum Archives (2009-): : Molecular-Cloning
391. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
392. Cloning large fragments - (reply: 9)
393. Transformation colonies does not contain insert - (reply: 3)
394. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
395. Need urgent advise.. - (reply: 3)
396. Cloning Mitochondrial Targeting Sequence - (reply: 4)
397. problem in cloning - (reply: 2)
398. TA cloning: ligation problem or toxic construct? - (reply: 4)
399. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
400. Do I need to sequence after digestion? - (reply: 9)
401. quick change mutagenesis...... - (reply: 15)
402. Problem with double digestion: one enzyme is not working - (reply: 8)
403. Transient Transfection Controls (Gateway Technology) - (reply: 4)
404. Problems with crossover PCR - (reply: 2)
405. gateway destination vector grows on kanamycin plates !!!!! - (reply: 1)
406. Two genes, same MCS - (reply: 1)
407. Including ATG in cloning? - (reply: 1)
408. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
409. Low copy vector for library generation - (reply: 2)
410. Can't find a recombinant after ligating - (reply: 4)
411. problems with plasmid miniprep - (reply: 8)
412. gene cloning - (reply: 1)
413. Is ligation really necessary? - (reply: 6)
414. problem with ligation of linker/adaptor - (reply: 2)
415. GFP -weak signal -why? - (reply: 1)
416. Ligation troubleshooting! Please help! - (reply: 5)
417. Truncated gene Cloning - (reply: 1)
418. Screening for ligation and transformation result - (reply: 2)
419. Problem with 3 way ligation - (reply: 2)
420. Query regarding primers for quick change mutagenesis - (reply: 3)