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Top : New Forum Archives (2009-): : Molecular-Cloning
391. Problems with designing an experiment in identifying mutations of particular gen - (reply: 5)
392. recombinant protein form - (reply: 2)
393. restricted PCR plasmid runs slower - (reply: 2)
394. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
395. High frequency of vector religation - (reply: 10)
396. Cloned but not expressing! - (reply: 8)
397. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
398. DNA ligation and trasformation - (reply: 6)
399. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
400. Storing NovaBlue Transformation Reactions in SOC - (reply: 1)
401. How to set up simultaneous digestion? - (reply: 3)
402. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
403. Problem with ligation using pJET and pGEM for clone library - (reply: 11)
404. Freeze transformation mixture? - (reply: 2)
405. Cutting my plasmid made it larger - (reply: 7)
406. The Issue of Freezing and Using colonies of Tranformed DH5a - (reply: 7)
407. Amplification of CAG promoter problems - (reply: 4)
408. restriction enzyme - (reply: 2)
409. Recreate original plasmid by cutting out insert - (reply: 2)
410. Colony-PCR workaround - (reply: 3)
411. I have a 96 tandem array repeat clone but need half of them in new clone - (reply: 4)
412. Can I pause a bacterial culture at 4C - (reply: 2)
413. TOPO TA CLONING - LIGATION STEP - (reply: 9)
414. posting sequences of plasmid? - (reply: 3)
415. Restriction Enzyme Digest not working - (reply: 2)
416. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 1)
417. Incomplete digestion of plasmid with single enzyme - (reply: 1)
418. problem with plasmid digestion - (reply: 5)
419. Difficult Ligation - Details Inside - (reply: 9)
420. pET 32 (+) - (reply: 1)