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Top : New Forum Archives (2009-): : Molecular-Cloning
391. Directions of genes expression illustrations - (reply: 5)
392. Plasmid DNA at RT for a few days - (reply: 4)
393. TOPO TA Cloning - (reply: 1)
394. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
395. No insert in subcloning - (reply: 3)
396. Digest does not confirm presence of fragment - (reply: 5)
397. Multiway ligation problems: pBS + 3 fragments: bad pattern - (reply: 1)
398. pGL3 Promoter replacing TSS bases - (reply: 1)
399. lipofectamine 2000:DNA complex stability - (reply: 2)
400. Problems with Ecoli glycerol stock - (reply: 6)
401. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
402. CIAP dilution - (reply: 5)
403. Problems in RNAi construction by using pUC19 - (reply: 3)
404. Unable to clone in E. coli cells - (reply: 3)
405. Why tranform using DH5 alpha -> SM10 λpir?? - (reply: 3)
406. plasmid rearrangement or sabotage - (reply: 2)
407. pLDR20 Vector - (reply: 1)
408. surprising behavior of transformants needs immediate advice! - (reply: 8)
409. cloning antisense promoter in pGL3Basic - (reply: 2)
410. PhiX174 DNA - (reply: 1)
411. Competent bacterial cells - (reply: 2)
412. Replacing puramycin in a pLKO1.puro - (reply: 1)
413. colony cracking for screening of positive clones - (reply: 2)
414. deletion of large fregments - (reply: 3)
415. Linearising plasmid before transfection? - (reply: 2)
416. Smear in plasmid sample - (reply: 9)
417. about a book - (reply: 1)
418. Cloning Problem! I need help - (reply: 3)
419. Help designing primers for use in In-fushion cloning - (reply: 4)
420. Repeat of desired mutation in cloning product - (reply: 1)