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Top : New Forum Archives (2009-): : Molecular-Cloning
631. Methods of plasmid transformation? only heat shock? - (reply: 1)
632. Digestion - (reply: 2)
633. Digestion of Genomic DNA - (reply: 1)
634. Isolation of a Gene of Interest for Subcloning - (reply: 8)
635. E.coli grow on neg. control - (reply: 1)
636. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
637. Need help troubleshooting digest. - (reply: 1)
638. Competent cells question: JM101 vs DH5alpha - (reply: 2)
639. How to avoid plasmid instability - (reply: 4)
640. Question Help - (reply: 1)
641. Shear-induced damage of plasmid by vortexing - (reply: 1)
642. Problems with blunt end ligation - (reply: 3)
643. Problem with Transforming E. coli DH10Bac - (reply: 8)
644. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
645. Heat inactivate ligase - (reply: 5)
646. DMSO stock of overnight bacterial cultures - (reply: 1)
647. Recommended competent cell for transformation of large plasmids? - (reply: 4)
648. Prolem in screening of hygromycin concentration - (reply: 1)
649. Primers for Introduction of new restriction sites to a vector - (reply: 3)
650. problem in cloning involving partial digestion - (reply: 1)
651. Why perform restriction enzyme digestion again? - (reply: 1)
652. Cotransient Transfections DNA Requirements - (reply: 3)
653. which software to draw the scheme of cloning strategy - (reply: 5)
654. Help with restriction analysis - (reply: 1)
655. Sequencing of PCR product - (reply: 4)
656. KanR sequence - (reply: 2)
657. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
658. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
659. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
660. Plasmid digestion after transformation - (reply: 4)