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Top : New Forum Archives (2009-): : Molecular-Cloning
631. Plasmid digestion after transformation - (reply: 4)
632. Blunt end ligation insert:vector ratio - (reply: 1)
633. PCRed on restriction sites -- how do I purify it? - (reply: 8)
634. Puzzle about plamid and its digest, plz help - (reply: 8)
635. Smear above plasmid dna - (reply: 3)
636. restriction digestion buffers contaminated with dnase - (reply: 3)
637. Bacterial Induction - (reply: 1)
638. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
639. smear restriction digest - (reply: 1)
640. How to set the Sonicator to 20%amplitude? - (reply: 3)
641. No DNA after midiprep - (reply: 2)
642. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
643. Is Kozak's full sequence necessary? or just ACCATG is enough? - (reply: 4)
644. how to store LB-agar-antibiotic- X-gal-IPTG plate - (reply: 3)
645. Impact of primer sequence on TA cloning - (reply: 3)
646. Maximum length of cDNA from RT reaction - (reply: 6)
647. overlapping PCR protocol - (reply: 12)
648. cloning an AT rich gene - (reply: 3)
649. Cloning two genes in the same plasmid - (reply: 4)
650. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
651. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
652. Problem: Cloning DNA shuffled band - (reply: 2)
653. shRNA to primary keratinocytes - (reply: 1)
654. Enzymetic digestion with ApaI and HincII - (reply: 2)
655. Ligation gel picture interpretation - (reply: 4)
656. strange subcloning problem - (reply: 9)
657. Cloned sequence is not predicted size by western blot - (reply: 1)
658. Ligation of insert to itself problem - (reply: 3)
659. double digest problem - (reply: 3)
660. Difficulty in expressing fusion protein - (reply: 1)