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Top : New Forum Archives (2009-): : Molecular-Cloning
631. Ligation needed after SDM with PfuX? - (reply: 3)
632. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
633. Cloning promoter (overlap with another gene!) - (reply: 3)
634. bacterial transfeomation - (reply: 2)
635. Loss of insert? - (reply: 1)
636. Sequencing trouble - (reply: 7)
637. Platinum-A cell trasnfection toruble - (reply: 1)
638. Cloning a promoter - (reply: 2)
639. pcDNA 3.1 His Expression - (reply: 2)
640. Cloning two fragments into a vector - (reply: 3)
641. confirmation of insert in to vector - (reply: 3)
642. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
643. Question about Molar ratio - (reply: 4)
644. Ligation problem - (reply: 16)
645. double digestion with sac1, hindIII - (reply: 3)
646. self-ligation Anneled oligos - (reply: 7)
647. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
648. Looking for a GFP plasmid (non-amp) - (reply: 3)
649. clonning problem (recombined by-products) - (reply: 8)
650. Genecloning three types of molecules? - (reply: 11)
651. help in digesting DNA fragment using restriction enzyme - (reply: 2)
652. Tansformation fails ,how can i make it ? - (reply: 3)
653. pelB leader - how to use - (reply: 1)
654. Transformation bacteria heat chock and incubation? - (reply: 2)
655. useing a uniqeu enzyme for both insert and vector digestion - (reply: 14)
656. ligation of pcr product into expression vector - (reply: 4)
657. amp plates containing xgal/iptg with expression vector - (reply: 6)
658. Strange Vector Problems - (reply: 3)
659. white colonies - (reply: 2)
660. how to design a kozak sequence in front of the start codon - (reply: 1)