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Top : New Forum Archives (2009-): : Molecular-Cloning
631. Digestion - (reply: 2)
632. Digestion of Genomic DNA - (reply: 1)
633. Isolation of a Gene of Interest for Subcloning - (reply: 8)
634. E.coli grow on neg. control - (reply: 1)
635. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
636. Need help troubleshooting digest. - (reply: 1)
637. Competent cells question: JM101 vs DH5alpha - (reply: 2)
638. How to avoid plasmid instability - (reply: 4)
639. Question Help - (reply: 1)
640. Shear-induced damage of plasmid by vortexing - (reply: 1)
641. Problems with blunt end ligation - (reply: 3)
642. Problem with Transforming E. coli DH10Bac - (reply: 8)
643. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
644. Heat inactivate ligase - (reply: 5)
645. DMSO stock of overnight bacterial cultures - (reply: 1)
646. Recommended competent cell for transformation of large plasmids? - (reply: 4)
647. Prolem in screening of hygromycin concentration - (reply: 1)
648. Primers for Introduction of new restriction sites to a vector - (reply: 3)
649. problem in cloning involving partial digestion - (reply: 1)
650. Why perform restriction enzyme digestion again? - (reply: 1)
651. Cotransient Transfections DNA Requirements - (reply: 3)
652. which software to draw the scheme of cloning strategy - (reply: 5)
653. Help with restriction analysis - (reply: 1)
654. Sequencing of PCR product - (reply: 4)
655. KanR sequence - (reply: 2)
656. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
657. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
658. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
659. Plasmid digestion after transformation - (reply: 4)
660. Blunt end ligation insert:vector ratio - (reply: 1)