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Top : New Forum Archives (2009-): : Molecular-Cloning
631. cloning into Pgem t easy - (reply: 1)
632. How to get pure protein of a gene - (reply: 3)
633. How to insert genes into chromosome of E. coli - (reply: 1)
634. Gene synthesis - (reply: 2)
635. I canīt obtain the amount of DNA required for ligation of blunt ends - (reply: 2)
636. Pls help me with this plasmid!!! - (reply: 1)
637. Problems with Restriction Digest Results - (reply: 7)
638. Methods of plasmid transformation? only heat shock? - (reply: 1)
639. Digestion - (reply: 2)
640. Digestion of Genomic DNA - (reply: 1)
641. Isolation of a Gene of Interest for Subcloning - (reply: 8)
642. E.coli grow on neg. control - (reply: 1)
643. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
644. Need help troubleshooting digest. - (reply: 1)
645. Competent cells question: JM101 vs DH5alpha - (reply: 2)
646. How to avoid plasmid instability - (reply: 4)
647. Question Help - (reply: 1)
648. Shear-induced damage of plasmid by vortexing - (reply: 1)
649. Problems with blunt end ligation - (reply: 3)
650. Problem with Transforming E. coli DH10Bac - (reply: 8)
651. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
652. Heat inactivate ligase - (reply: 5)
653. DMSO stock of overnight bacterial cultures - (reply: 1)
654. Recommended competent cell for transformation of large plasmids? - (reply: 4)
655. Prolem in screening of hygromycin concentration - (reply: 1)
656. Primers for Introduction of new restriction sites to a vector - (reply: 3)
657. problem in cloning involving partial digestion - (reply: 1)
658. Why perform restriction enzyme digestion again? - (reply: 1)
659. Cotransient Transfections DNA Requirements - (reply: 3)
660. which software to draw the scheme of cloning strategy - (reply: 5)