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Top : New Forum Archives (2009-): : Molecular-Cloning
631. Pls help me with this plasmid!!! - (reply: 1)
632. Problems with Restriction Digest Results - (reply: 7)
633. Methods of plasmid transformation? only heat shock? - (reply: 1)
634. Digestion - (reply: 2)
635. Digestion of Genomic DNA - (reply: 1)
636. Isolation of a Gene of Interest for Subcloning - (reply: 8)
637. E.coli grow on neg. control - (reply: 1)
638. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
639. Need help troubleshooting digest. - (reply: 1)
640. Competent cells question: JM101 vs DH5alpha - (reply: 2)
641. How to avoid plasmid instability - (reply: 4)
642. Question Help - (reply: 1)
643. Shear-induced damage of plasmid by vortexing - (reply: 1)
644. Problems with blunt end ligation - (reply: 3)
645. Problem with Transforming E. coli DH10Bac - (reply: 8)
646. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
647. Heat inactivate ligase - (reply: 5)
648. DMSO stock of overnight bacterial cultures - (reply: 1)
649. Recommended competent cell for transformation of large plasmids? - (reply: 4)
650. Prolem in screening of hygromycin concentration - (reply: 1)
651. Primers for Introduction of new restriction sites to a vector - (reply: 3)
652. problem in cloning involving partial digestion - (reply: 1)
653. Why perform restriction enzyme digestion again? - (reply: 1)
654. Cotransient Transfections DNA Requirements - (reply: 3)
655. which software to draw the scheme of cloning strategy - (reply: 5)
656. Help with restriction analysis - (reply: 1)
657. Sequencing of PCR product - (reply: 4)
658. KanR sequence - (reply: 2)
659. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
660. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)