Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
631. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
632. Why don't I get the expected entry clones when I use the gateway system?? - (reply: 2)
633. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
634. cloning into Pgem t easy - (reply: 1)
635. How to get pure protein of a gene - (reply: 3)
636. How to insert genes into chromosome of E. coli - (reply: 1)
637. Gene synthesis - (reply: 2)
638. I canīt obtain the amount of DNA required for ligation of blunt ends - (reply: 2)
639. Pls help me with this plasmid!!! - (reply: 1)
640. Problems with Restriction Digest Results - (reply: 7)
641. Methods of plasmid transformation? only heat shock? - (reply: 1)
642. Digestion - (reply: 2)
643. Digestion of Genomic DNA - (reply: 1)
644. Isolation of a Gene of Interest for Subcloning - (reply: 8)
645. E.coli grow on neg. control - (reply: 1)
646. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
647. Need help troubleshooting digest. - (reply: 1)
648. Competent cells question: JM101 vs DH5alpha - (reply: 2)
649. How to avoid plasmid instability - (reply: 4)
650. Question Help - (reply: 1)
651. Shear-induced damage of plasmid by vortexing - (reply: 1)
652. Problems with blunt end ligation - (reply: 3)
653. Problem with Transforming E. coli DH10Bac - (reply: 8)
654. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
655. Heat inactivate ligase - (reply: 5)
656. DMSO stock of overnight bacterial cultures - (reply: 1)
657. Recommended competent cell for transformation of large plasmids? - (reply: 4)
658. Prolem in screening of hygromycin concentration - (reply: 1)
659. Primers for Introduction of new restriction sites to a vector - (reply: 3)
660. problem in cloning involving partial digestion - (reply: 1)