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Top : New Forum Archives (2009-): : Molecular-Cloning
631. Is Kozak's full sequence necessary? or just ACCATG is enough? - (reply: 4)
632. how to store LB-agar-antibiotic- X-gal-IPTG plate - (reply: 3)
633. Impact of primer sequence on TA cloning - (reply: 3)
634. Maximum length of cDNA from RT reaction - (reply: 6)
635. overlapping PCR protocol - (reply: 12)
636. cloning an AT rich gene - (reply: 3)
637. Cloning two genes in the same plasmid - (reply: 4)
638. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
639. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
640. Problem: Cloning DNA shuffled band - (reply: 2)
641. shRNA to primary keratinocytes - (reply: 1)
642. Enzymetic digestion with ApaI and HincII - (reply: 2)
643. Ligation gel picture interpretation - (reply: 4)
644. strange subcloning problem - (reply: 9)
645. Cloned sequence is not predicted size by western blot - (reply: 1)
646. Ligation of insert to itself problem - (reply: 3)
647. double digest problem - (reply: 3)
648. Difficulty in expressing fusion protein - (reply: 1)
649. Plasmid recovery problem - (reply: 3)
650. Restriction Digest Problem - (reply: 5)
651. Recombination protocol needed - (reply: 2)
652. Cloning problem using pET32a vector - (reply: 3)
653. Fixing SNPs after cloning - (reply: 5)
654. Electroporated plasmid loses the insert - (reply: 8)
655. Recombination due to overselective plate? - (reply: 11)
656. Help with ligation - (reply: 2)
657. emty vector cloning - (reply: 3)
658. RE site on complimentary strand of plasmid? - (reply: 1)
659. LENTIVIRUS transfection efficiency - (reply: 1)
660. Double digest after cloning doesn't give gene - (reply: 6)