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Top : New Forum Archives (2009-): : Molecular-Cloning
631. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
632. Template DNA (plasmid) concentration for PCR - (reply: 4)
633. Ligation of 16kb PCR product - (reply: 5)
634. stable transfection - (reply: 1)
635. How to choose the right plasmids for certain types of cell? - (reply: 1)
636. Ligation needed after SDM with PfuX? - (reply: 3)
637. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
638. Cloning promoter (overlap with another gene!) - (reply: 3)
639. bacterial transfeomation - (reply: 2)
640. Loss of insert? - (reply: 1)
641. Sequencing trouble - (reply: 7)
642. Platinum-A cell trasnfection toruble - (reply: 1)
643. Cloning a promoter - (reply: 2)
644. pcDNA 3.1 His Expression - (reply: 2)
645. Cloning two fragments into a vector - (reply: 3)
646. confirmation of insert in to vector - (reply: 3)
647. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
648. Question about Molar ratio - (reply: 4)
649. Ligation problem - (reply: 16)
650. double digestion with sac1, hindIII - (reply: 3)
651. self-ligation Anneled oligos - (reply: 7)
652. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
653. Looking for a GFP plasmid (non-amp) - (reply: 3)
654. clonning problem (recombined by-products) - (reply: 8)
655. Genecloning three types of molecules? - (reply: 11)
656. help in digesting DNA fragment using restriction enzyme - (reply: 2)
657. Tansformation fails ,how can i make it ? - (reply: 3)
658. pelB leader - how to use - (reply: 1)
659. Transformation bacteria heat chock and incubation? - (reply: 2)
660. useing a uniqeu enzyme for both insert and vector digestion - (reply: 14)