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Top : New Forum Archives (2009-): : Molecular-Cloning
631. Why don't I get the expected entry clones when I use the gateway system?? - (reply: 2)
632. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
633. cloning into Pgem t easy - (reply: 1)
634. How to get pure protein of a gene - (reply: 3)
635. How to insert genes into chromosome of E. coli - (reply: 1)
636. Gene synthesis - (reply: 2)
637. I canīt obtain the amount of DNA required for ligation of blunt ends - (reply: 2)
638. Pls help me with this plasmid!!! - (reply: 1)
639. Problems with Restriction Digest Results - (reply: 7)
640. Methods of plasmid transformation? only heat shock? - (reply: 1)
641. Digestion - (reply: 2)
642. Digestion of Genomic DNA - (reply: 1)
643. Isolation of a Gene of Interest for Subcloning - (reply: 8)
644. E.coli grow on neg. control - (reply: 1)
645. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
646. Need help troubleshooting digest. - (reply: 1)
647. Competent cells question: JM101 vs DH5alpha - (reply: 2)
648. How to avoid plasmid instability - (reply: 4)
649. Question Help - (reply: 1)
650. Shear-induced damage of plasmid by vortexing - (reply: 1)
651. Problems with blunt end ligation - (reply: 3)
652. Problem with Transforming E. coli DH10Bac - (reply: 8)
653. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
654. Heat inactivate ligase - (reply: 5)
655. DMSO stock of overnight bacterial cultures - (reply: 1)
656. Recommended competent cell for transformation of large plasmids? - (reply: 4)
657. Prolem in screening of hygromycin concentration - (reply: 1)
658. Primers for Introduction of new restriction sites to a vector - (reply: 3)
659. problem in cloning involving partial digestion - (reply: 1)
660. Why perform restriction enzyme digestion again? - (reply: 1)