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Top : New Forum Archives (2009-): : Molecular-Cloning
631. Ligation of double insert into pET31 failing - (reply: 1)
632. Homemade MiniPreps for Plasmid Purification - (reply: 1)
633. Agar has dried out during cultivation - (reply: 1)
634. Gateway cloning vector compatibility - (reply: 2)
635. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
636. Cloning of IL8-promoter - (reply: 3)
637. I know the mRNA complete CDS, but How can I obtain the full length cDNA of this - (reply: 3)
638. Template DNA (plasmid) concentration for PCR - (reply: 4)
639. Ligation of 16kb PCR product - (reply: 5)
640. stable transfection - (reply: 1)
641. How to choose the right plasmids for certain types of cell? - (reply: 1)
642. Ligation needed after SDM with PfuX? - (reply: 3)
643. For E. coli translation of an mRNA, do you need space between the stop codon and - (reply: 2)
644. Cloning promoter (overlap with another gene!) - (reply: 3)
645. bacterial transfeomation - (reply: 2)
646. Loss of insert? - (reply: 1)
647. Sequencing trouble - (reply: 7)
648. Platinum-A cell trasnfection toruble - (reply: 1)
649. Cloning a promoter - (reply: 2)
650. pcDNA 3.1 His Expression - (reply: 2)
651. Cloning two fragments into a vector - (reply: 3)
652. confirmation of insert in to vector - (reply: 3)
653. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
654. Question about Molar ratio - (reply: 4)
655. Ligation problem - (reply: 16)
656. double digestion with sac1, hindIII - (reply: 3)
657. self-ligation Anneled oligos - (reply: 7)
658. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
659. Looking for a GFP plasmid (non-amp) - (reply: 3)
660. clonning problem (recombined by-products) - (reply: 8)