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631. HELP!Mini preps resulting in low yields where previously high. - (reply: 16)
632. New to sequencing, primer design - (reply: 2)
633. ta cloning problem - (reply: 2)
634. producing recombinant -one gene plus strand/other gene complemenary and reverse - (reply: 3)
635. pBR322 restriction digest - PBR322 digest with EcoRV and BstZ17I (reply: 20)
636. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
637. Storage of Bacterial Culture at 4 degrees - (reply: 2)
638. Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
639. rna isolation - (reply: 1)
640. Cloning strategy for a membrane protein - did I do anything wrongly? (reply: 2)
641. Molecular Cloning od Recombinant DNA - (reply: 3)
642. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
643. looking for binding site - software? (reply: 1)
644. Insertion of loxp/avoiding SINE - (reply: 1)
645. Question: Ligation Problem.... - (reply: 2)
646. Plasmid-Stability? Fragment-Deletion? I'm in dire need to identify and solve - (reply: 20)
647. Topo directional cloning - pENTR/D-TOPO (reply: 1)
648. trouble in TOPO TA sequencing - (reply: 1)
649. Large DNA extraction from an agarose gel - (reply: 8)
650. Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
651. primer Tm is too high, how tu get pcr product - help! (reply: 21)
652. Ligation problem, religation of backbone without any insert? - (reply: 3)
653. problem with pLK01 plasmid ligation - (reply: 2)
654. Mini prep streaking - basic question (reply: 3)
655. In-fusion joining few parts together - possible? ideas? (reply: 3)
656. TOPO cloning problem - (reply: 4)
657. How to delete a domain in a gene? - cloned plasmid (reply: 12)
658. Red/ET recombination - (reply: 2)
659. multiple origins of replication - (reply: 2)
660. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
632. New to sequencing, primer design - (reply: 2)
633. ta cloning problem - (reply: 2)
634. producing recombinant -one gene plus strand/other gene complemenary and reverse - (reply: 3)
635. pBR322 restriction digest - PBR322 digest with EcoRV and BstZ17I (reply: 20)
636. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
637. Storage of Bacterial Culture at 4 degrees - (reply: 2)
638. Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
639. rna isolation - (reply: 1)
640. Cloning strategy for a membrane protein - did I do anything wrongly? (reply: 2)
641. Molecular Cloning od Recombinant DNA - (reply: 3)
642. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
643. looking for binding site - software? (reply: 1)
644. Insertion of loxp/avoiding SINE - (reply: 1)
645. Question: Ligation Problem.... - (reply: 2)
646. Plasmid-Stability? Fragment-Deletion? I'm in dire need to identify and solve - (reply: 20)
647. Topo directional cloning - pENTR/D-TOPO (reply: 1)
648. trouble in TOPO TA sequencing - (reply: 1)
649. Large DNA extraction from an agarose gel - (reply: 8)
650. Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
651. primer Tm is too high, how tu get pcr product - help! (reply: 21)
652. Ligation problem, religation of backbone without any insert? - (reply: 3)
653. problem with pLK01 plasmid ligation - (reply: 2)
654. Mini prep streaking - basic question (reply: 3)
655. In-fusion joining few parts together - possible? ideas? (reply: 3)
656. TOPO cloning problem - (reply: 4)
657. How to delete a domain in a gene? - cloned plasmid (reply: 12)
658. Red/ET recombination - (reply: 2)
659. multiple origins of replication - (reply: 2)
660. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)