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Top : New Forum Archives (2009-): : Molecular-Cloning
631. Question Help - (reply: 1)
632. Shear-induced damage of plasmid by vortexing - (reply: 1)
633. Problems with blunt end ligation - (reply: 3)
634. Problem with Transforming E. coli DH10Bac - (reply: 8)
635. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
636. Heat inactivate ligase - (reply: 5)
637. DMSO stock of overnight bacterial cultures - (reply: 1)
638. Recommended competent cell for transformation of large plasmids? - (reply: 4)
639. Prolem in screening of hygromycin concentration - (reply: 1)
640. Primers for Introduction of new restriction sites to a vector - (reply: 3)
641. problem in cloning involving partial digestion - (reply: 1)
642. Why perform restriction enzyme digestion again? - (reply: 1)
643. Cotransient Transfections DNA Requirements - (reply: 3)
644. which software to draw the scheme of cloning strategy - (reply: 5)
645. Help with restriction analysis - (reply: 1)
646. Sequencing of PCR product - (reply: 4)
647. KanR sequence - (reply: 2)
648. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
649. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
650. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
651. Plasmid digestion after transformation - (reply: 4)
652. Blunt end ligation insert:vector ratio - (reply: 1)
653. PCRed on restriction sites -- how do I purify it? - (reply: 8)
654. Puzzle about plamid and its digest, plz help - (reply: 8)
655. Smear above plasmid dna - (reply: 3)
656. restriction digestion buffers contaminated with dnase - (reply: 3)
657. Bacterial Induction - (reply: 1)
658. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
659. smear restriction digest - (reply: 1)
660. How to set the Sonicator to 20%amplitude? - (reply: 3)