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Top : New Forum Archives (2009-): : Molecular-Cloning
631. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
632. Plasmid digestion after transformation - (reply: 4)
633. Blunt end ligation insert:vector ratio - (reply: 1)
634. PCRed on restriction sites -- how do I purify it? - (reply: 8)
635. Puzzle about plamid and its digest, plz help - (reply: 8)
636. Smear above plasmid dna - (reply: 3)
637. restriction digestion buffers contaminated with dnase - (reply: 3)
638. Bacterial Induction - (reply: 1)
639. purification of digested vector by agarose eletrophoresis problem - (reply: 4)
640. smear restriction digest - (reply: 1)
641. How to set the Sonicator to 20%amplitude? - (reply: 3)
642. No DNA after midiprep - (reply: 2)
643. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
644. Is Kozak's full sequence necessary? or just ACCATG is enough? - (reply: 4)
645. how to store LB-agar-antibiotic- X-gal-IPTG plate - (reply: 3)
646. Impact of primer sequence on TA cloning - (reply: 3)
647. Maximum length of cDNA from RT reaction - (reply: 6)
648. overlapping PCR protocol - (reply: 12)
649. cloning an AT rich gene - (reply: 3)
650. Cloning two genes in the same plasmid - (reply: 4)
651. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
652. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
653. Problem: Cloning DNA shuffled band - (reply: 2)
654. shRNA to primary keratinocytes - (reply: 1)
655. Enzymetic digestion with ApaI and HincII - (reply: 2)
656. Ligation gel picture interpretation - (reply: 4)
657. strange subcloning problem - (reply: 9)
658. Cloned sequence is not predicted size by western blot - (reply: 1)
659. Ligation of insert to itself problem - (reply: 3)
660. double digest problem - (reply: 3)