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Top : New Forum Archives (2009-): : Molecular-Cloning
631. Problems with Restriction Digest Results - (reply: 7)
632. Methods of plasmid transformation? only heat shock? - (reply: 1)
633. Digestion - (reply: 2)
634. Digestion of Genomic DNA - (reply: 1)
635. Isolation of a Gene of Interest for Subcloning - (reply: 8)
636. E.coli grow on neg. control - (reply: 1)
637. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
638. Need help troubleshooting digest. - (reply: 1)
639. Competent cells question: JM101 vs DH5alpha - (reply: 2)
640. How to avoid plasmid instability - (reply: 4)
641. Question Help - (reply: 1)
642. Shear-induced damage of plasmid by vortexing - (reply: 1)
643. Problems with blunt end ligation - (reply: 3)
644. Problem with Transforming E. coli DH10Bac - (reply: 8)
645. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
646. Heat inactivate ligase - (reply: 5)
647. DMSO stock of overnight bacterial cultures - (reply: 1)
648. Recommended competent cell for transformation of large plasmids? - (reply: 4)
649. Prolem in screening of hygromycin concentration - (reply: 1)
650. Primers for Introduction of new restriction sites to a vector - (reply: 3)
651. problem in cloning involving partial digestion - (reply: 1)
652. Why perform restriction enzyme digestion again? - (reply: 1)
653. Cotransient Transfections DNA Requirements - (reply: 3)
654. which software to draw the scheme of cloning strategy - (reply: 5)
655. Help with restriction analysis - (reply: 1)
656. Sequencing of PCR product - (reply: 4)
657. KanR sequence - (reply: 2)
658. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
659. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
660. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)