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Top : New Forum Archives (2009-): : Molecular-Cloning
631. 12.4kb vector 6.7kb insert - (reply: 2)
632. Stable transfection - (reply: 2)
633. Using normal competent cells in electroporation method? - (reply: 1)
634. an effective way to do a yeast colony pcr - (reply: 2)
635. pGEM-T Easy vector contamination - (reply: 2)
636. recover old transformed DH5 - (reply: 1)
637. NZY+Broth can be replaced by LB Broth in mutagenesis exp - (reply: 2)
638. oligo(dt) 15 vs random primers - (reply: 3)
639. I need bynary vector pART27 - (reply: 1)
640. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
641. Why don't I get the expected entry clones when I use the gateway system?? - (reply: 2)
642. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
643. cloning into Pgem t easy - (reply: 1)
644. How to get pure protein of a gene - (reply: 3)
645. How to insert genes into chromosome of E. coli - (reply: 1)
646. Gene synthesis - (reply: 2)
647. I canīt obtain the amount of DNA required for ligation of blunt ends - (reply: 2)
648. Pls help me with this plasmid!!! - (reply: 1)
649. Problems with Restriction Digest Results - (reply: 7)
650. Methods of plasmid transformation? only heat shock? - (reply: 1)
651. Digestion - (reply: 2)
652. Digestion of Genomic DNA - (reply: 1)
653. Isolation of a Gene of Interest for Subcloning - (reply: 8)
654. E.coli grow on neg. control - (reply: 1)
655. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
656. Need help troubleshooting digest. - (reply: 1)
657. Competent cells question: JM101 vs DH5alpha - (reply: 2)
658. How to avoid plasmid instability - (reply: 4)
659. Question Help - (reply: 1)
660. Shear-induced damage of plasmid by vortexing - (reply: 1)