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Top : New Forum Archives (2009-): : Molecular-Cloning
631. Cloning genes into plant vector - (reply: 1)
632. Do amino acids and his tag on vector affect protein of interest? - (reply: 4)
633. Three-way ligation in two steps? - (reply: 3)
634. How does circular, uncut pGEM-T migrate? - (reply: 2)
635. Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)
636. 3-way ligation of 2 annealed dsDNAs and pGEM-T Easy - (reply: 6)
637. Cloning App Released from Life Technologies - (reply: 2)
638. gateway cloning: is BP-cloning "reversible" by LR-reaction? - (reply: 2)
639. Dissolve IPTG in EtOH - (reply: 3)
640. problem with transient transfection - (reply: 6)
641. Recombinant construct looks smaller than empty vector - (reply: 3)
642. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
643. hygromycin concentration for e-coli selection - (reply: 1)
644. orientation of promoter into viral construct - (reply: 1)
645. problems with PCR confirmation of insert . HELP ! - (reply: 1)
646. "CpG methylation: Blocked by some combinations of overlapping" - (reply: 1)
647. Loss of promoter during ligation - (reply: 2)
648. Enzyme restriction - (reply: 8)
649. Colony PCR Problem!!!! - (reply: 5)
650. Removing unwanted sequence from pUC19 - (reply: 4)
651. transformation fails, bacteria grows in control plate - (reply: 6)
652. competentce cell, cloning - (reply: 2)
653. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
654. co-transformation - (reply: 3)
655. pcr clean-up fail? - (reply: 1)
656. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
657. sequential cloning of multiple PCR products - how to do? (reply: 3)
658. Cloning large gene - (reply: 3)
659. Glycosilated peptide's cDNA cloning - (reply: 3)
660. pWM91 cloning problem - (reply: 4)