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Top : New Forum Archives (2009-): : Molecular-Cloning
631. How to insert genes into chromosome of E. coli - (reply: 1)
632. Gene synthesis - (reply: 2)
633. I canīt obtain the amount of DNA required for ligation of blunt ends - (reply: 2)
634. Pls help me with this plasmid!!! - (reply: 1)
635. Problems with Restriction Digest Results - (reply: 7)
636. Methods of plasmid transformation? only heat shock? - (reply: 1)
637. Digestion - (reply: 2)
638. Digestion of Genomic DNA - (reply: 1)
639. Isolation of a Gene of Interest for Subcloning - (reply: 8)
640. E.coli grow on neg. control - (reply: 1)
641. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
642. Need help troubleshooting digest. - (reply: 1)
643. Competent cells question: JM101 vs DH5alpha - (reply: 2)
644. How to avoid plasmid instability - (reply: 4)
645. Question Help - (reply: 1)
646. Shear-induced damage of plasmid by vortexing - (reply: 1)
647. Problems with blunt end ligation - (reply: 3)
648. Problem with Transforming E. coli DH10Bac - (reply: 8)
649. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
650. Heat inactivate ligase - (reply: 5)
651. DMSO stock of overnight bacterial cultures - (reply: 1)
652. Recommended competent cell for transformation of large plasmids? - (reply: 4)
653. Prolem in screening of hygromycin concentration - (reply: 1)
654. Primers for Introduction of new restriction sites to a vector - (reply: 3)
655. problem in cloning involving partial digestion - (reply: 1)
656. Why perform restriction enzyme digestion again? - (reply: 1)
657. Cotransient Transfections DNA Requirements - (reply: 3)
658. which software to draw the scheme of cloning strategy - (reply: 5)
659. Help with restriction analysis - (reply: 1)
660. Sequencing of PCR product - (reply: 4)