|
1321. Lb-kan plates life - How long can they be used? (reply: 2)
1322. LR reaction - Cloning into a expression vector (reply: 1)
1323. TOPO-Transformation Problem - (reply: 4)
1324. Checking Plasmid Expression - (reply: 1)
1325. designing primers for genes not sequenced yet - (reply: 2)
1326. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
1327. Cloning Problem - (reply: 3)
1328. AdEasy Vector System: Why PacI digestion? - (reply: 1)
1329. Can transormations be incubated at less than 37 degrees after heat shock? - What to do when the shaker is in use (reply: 4)
1330. cloning large insert in pGEM-T easy vector - Does TA cloning work for big inserts? (reply: 2)
1331. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
1332. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
1333. Restriction buffer composition - (reply: 2)
1334. same weird weird staph transformation results - any idea what is going on? (reply: 12)
1335. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
1336. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
1337. Yet another ligation problem - (reply: 4)
1338. Problems with Quick change lightning site directed mutagenesis kit(STRATAGENE) - (reply: 2)
1339. Promoter Cloning for Luciferase Assay - (reply: 3)
1340. Creating mutants - (reply: 3)
1341. cloning protocol - (reply: 1)
1342. Ligation Time and Temp - (reply: 4)
1343. Miniprep - (reply: 3)
1344. PCR and then ligation - (reply: 7)
1345. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
1346. 3 way ligation help needed - (reply: 10)
1347. Cloning and Expression Method Survey - Get some ideas from you all (reply: 1)
1348. Cloning woes - Running out of ideas! - (reply: 7)
1349. What fields of research other than microbiology work with bacteria and - (reply: 1)
1350. How to inoculate bacteria? - (reply: 10)
1322. LR reaction - Cloning into a expression vector (reply: 1)
1323. TOPO-Transformation Problem - (reply: 4)
1324. Checking Plasmid Expression - (reply: 1)
1325. designing primers for genes not sequenced yet - (reply: 2)
1326. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
1327. Cloning Problem - (reply: 3)
1328. AdEasy Vector System: Why PacI digestion? - (reply: 1)
1329. Can transormations be incubated at less than 37 degrees after heat shock? - What to do when the shaker is in use (reply: 4)
1330. cloning large insert in pGEM-T easy vector - Does TA cloning work for big inserts? (reply: 2)
1331. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
1332. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
1333. Restriction buffer composition - (reply: 2)
1334. same weird weird staph transformation results - any idea what is going on? (reply: 12)
1335. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
1336. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
1337. Yet another ligation problem - (reply: 4)
1338. Problems with Quick change lightning site directed mutagenesis kit(STRATAGENE) - (reply: 2)
1339. Promoter Cloning for Luciferase Assay - (reply: 3)
1340. Creating mutants - (reply: 3)
1341. cloning protocol - (reply: 1)
1342. Ligation Time and Temp - (reply: 4)
1343. Miniprep - (reply: 3)
1344. PCR and then ligation - (reply: 7)
1345. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
1346. 3 way ligation help needed - (reply: 10)
1347. Cloning and Expression Method Survey - Get some ideas from you all (reply: 1)
1348. Cloning woes - Running out of ideas! - (reply: 7)
1349. What fields of research other than microbiology work with bacteria and - (reply: 1)
1350. How to inoculate bacteria? - (reply: 10)