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Top : New Forum Archives (2009-): : Molecular-Cloning
1321. What fields of research other than microbiology work with bacteria and - (reply: 1)
1322. How to inoculate bacteria? - (reply: 10)
1323. What retrovirus vetor can have 7.5kb insert - (reply: 1)
1324. need help in my Fse1 restriction ezyme - (reply: 4)
1325. Insert problems with TA cloning - (reply: 1)
1326. Surface expression by tansfection of CHO - (reply: 3)
1327. please help!!! with transformation - (reply: 10)
1328. sequencing primer: T7 or T7promoter? - (reply: 4)
1329. Unstable clone? - (reply: 1)
1330. Restriction enzymes - (reply: 3)
1331. Problems facing in cloning with pBI121 vector - (reply: 1)
1332. Failed Transformation of Tuner (DE3) cells - (reply: 1)
1333. Is my insert present??? - (reply: 17)
1334. Need help for restriction enzymes - (reply: 5)
1335. Heat inactivation/Gel purification or both? - Normally I purify from gel. Should I inactivate the enzymes? (reply: 2)
1336. Mini prep - (reply: 4)
1337. adding koazak sequence to primer - (reply: 1)
1338. DNA ligation - (reply: 2)
1339. adding C terminal tag to reverse primer - (reply: 1)
1340. glycerol stocks - (reply: 10)
1341. doubts in sequence submission to NCBI - (reply: 2)
1342. Concentration of DNA for Transformation - (reply: 1)
1343. Restriction digest - (reply: 7)
1344. Help! Double restriction digestion failed.... - (reply: 6)
1345. Kozak sequences.. - (reply: 1)
1346. Sequence management software (for Mac) - (reply: 5)
1347. ligation for 24 hrs in 4*C? - (reply: 3)
1348. Having problem establishing stable cell line...... - (reply: 5)
1349. Best method for Mutagenesis? - multisite mutagenesis (reply: 2)
1350. Contruction of Expression Construct - (reply: 1)