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1321. cloning protocol - (reply: 1)
1322. Ligation Time and Temp - (reply: 4)
1323. Miniprep - (reply: 3)
1324. PCR and then ligation - (reply: 7)
1325. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
1326. 3 way ligation help needed - (reply: 10)
1327. Cloning and Expression Method Survey - Get some ideas from you all (reply: 1)
1328. Cloning woes - Running out of ideas! - (reply: 7)
1329. What fields of research other than microbiology work with bacteria and - (reply: 1)
1330. How to inoculate bacteria? - (reply: 10)
1331. What retrovirus vetor can have 7.5kb insert - (reply: 1)
1332. need help in my Fse1 restriction ezyme - (reply: 4)
1333. Insert problems with TA cloning - (reply: 1)
1334. Surface expression by tansfection of CHO - (reply: 3)
1335. please help!!! with transformation - (reply: 10)
1336. sequencing primer: T7 or T7promoter? - (reply: 4)
1337. Unstable clone? - (reply: 1)
1338. Restriction enzymes - (reply: 3)
1339. Problems facing in cloning with pBI121 vector - (reply: 1)
1340. Failed Transformation of Tuner (DE3) cells - (reply: 1)
1341. Is my insert present??? - (reply: 17)
1342. Need help for restriction enzymes - (reply: 5)
1343. Heat inactivation/Gel purification or both? - Normally I purify from gel. Should I inactivate the enzymes? (reply: 2)
1344. Mini prep - (reply: 4)
1345. adding koazak sequence to primer - (reply: 1)
1346. DNA ligation - (reply: 2)
1347. adding C terminal tag to reverse primer - (reply: 1)
1348. glycerol stocks - (reply: 10)
1349. doubts in sequence submission to NCBI - (reply: 2)
1350. Concentration of DNA for Transformation - (reply: 1)
1322. Ligation Time and Temp - (reply: 4)
1323. Miniprep - (reply: 3)
1324. PCR and then ligation - (reply: 7)
1325. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
1326. 3 way ligation help needed - (reply: 10)
1327. Cloning and Expression Method Survey - Get some ideas from you all (reply: 1)
1328. Cloning woes - Running out of ideas! - (reply: 7)
1329. What fields of research other than microbiology work with bacteria and - (reply: 1)
1330. How to inoculate bacteria? - (reply: 10)
1331. What retrovirus vetor can have 7.5kb insert - (reply: 1)
1332. need help in my Fse1 restriction ezyme - (reply: 4)
1333. Insert problems with TA cloning - (reply: 1)
1334. Surface expression by tansfection of CHO - (reply: 3)
1335. please help!!! with transformation - (reply: 10)
1336. sequencing primer: T7 or T7promoter? - (reply: 4)
1337. Unstable clone? - (reply: 1)
1338. Restriction enzymes - (reply: 3)
1339. Problems facing in cloning with pBI121 vector - (reply: 1)
1340. Failed Transformation of Tuner (DE3) cells - (reply: 1)
1341. Is my insert present??? - (reply: 17)
1342. Need help for restriction enzymes - (reply: 5)
1343. Heat inactivation/Gel purification or both? - Normally I purify from gel. Should I inactivate the enzymes? (reply: 2)
1344. Mini prep - (reply: 4)
1345. adding koazak sequence to primer - (reply: 1)
1346. DNA ligation - (reply: 2)
1347. adding C terminal tag to reverse primer - (reply: 1)
1348. glycerol stocks - (reply: 10)
1349. doubts in sequence submission to NCBI - (reply: 2)
1350. Concentration of DNA for Transformation - (reply: 1)