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1321. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
1322. SDM - (reply: 3)
1323. inframe problem - (reply: 1)
1324. Touchdown PCR - (reply: 1)
1325. Adding A tails? - (reply: 3)
1326. What does the DH5alpha mean in E.coli DH5alpha bacteria - (reply: 10)
1327. Determine annealing temperature of primers - (reply: 14)
1328. Bacteria pellet has almost-black region - (reply: 10)
1329. no ligation !!!!! - (reply: 6)
1330. How to clean up restriction enzyme digestion - (reply: 8)
1331. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
1332. GPCR - (reply: 1)
1333. Gene Bridges Red/ET cloning - unexpected streptomycin resistance (reply: 4)
1334. finding the suitable RE - first timer (reply: 6)
1335. TOPO TA Problems - TOPO TA Problems (reply: 8)
1336. about antibiotic addition - (reply: 4)
1337. about chloramphenicol and L.lactis - (reply: 1)
1338. ligation - (reply: 5)
1339. Losing insert from pGEM? - (reply: 2)
1340. How to concentrate lenti or retroviral particules - (reply: 3)
1341. wierd clot in LB culture - (reply: 5)
1342. Please anybody can explain the cloning related to plant vector construction - Plant vector construction (reply: 5)
1343. Not getting enough colony - (reply: 4)
1344. Ligation problem - (reply: 9)
1345. New Technologies Real time PCR, cloning, microarray, sequencing - (reply: 3)
1346. about flanking sequnce - (reply: 3)
1347. Ligation issues - (reply: 4)
1348. messed up restriction sites - (reply: 3)
1349. annoying PCR cloning problem (season 2) - (reply: 5)
1350. Cloning Issues with Rest Digest/Ligation/Transformation - (reply: 2)
1322. SDM - (reply: 3)
1323. inframe problem - (reply: 1)
1324. Touchdown PCR - (reply: 1)
1325. Adding A tails? - (reply: 3)
1326. What does the DH5alpha mean in E.coli DH5alpha bacteria - (reply: 10)
1327. Determine annealing temperature of primers - (reply: 14)
1328. Bacteria pellet has almost-black region - (reply: 10)
1329. no ligation !!!!! - (reply: 6)
1330. How to clean up restriction enzyme digestion - (reply: 8)
1331. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
1332. GPCR - (reply: 1)
1333. Gene Bridges Red/ET cloning - unexpected streptomycin resistance (reply: 4)
1334. finding the suitable RE - first timer (reply: 6)
1335. TOPO TA Problems - TOPO TA Problems (reply: 8)
1336. about antibiotic addition - (reply: 4)
1337. about chloramphenicol and L.lactis - (reply: 1)
1338. ligation - (reply: 5)
1339. Losing insert from pGEM? - (reply: 2)
1340. How to concentrate lenti or retroviral particules - (reply: 3)
1341. wierd clot in LB culture - (reply: 5)
1342. Please anybody can explain the cloning related to plant vector construction - Plant vector construction (reply: 5)
1343. Not getting enough colony - (reply: 4)
1344. Ligation problem - (reply: 9)
1345. New Technologies Real time PCR, cloning, microarray, sequencing - (reply: 3)
1346. about flanking sequnce - (reply: 3)
1347. Ligation issues - (reply: 4)
1348. messed up restriction sites - (reply: 3)
1349. annoying PCR cloning problem (season 2) - (reply: 5)
1350. Cloning Issues with Rest Digest/Ligation/Transformation - (reply: 2)