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Top : New Forum Archives (2009-): : Molecular-Cloning
1321. Cloning woes - Running out of ideas! - (reply: 7)
1322. What fields of research other than microbiology work with bacteria and - (reply: 1)
1323. How to inoculate bacteria? - (reply: 10)
1324. What retrovirus vetor can have 7.5kb insert - (reply: 1)
1325. need help in my Fse1 restriction ezyme - (reply: 4)
1326. Insert problems with TA cloning - (reply: 1)
1327. Surface expression by tansfection of CHO - (reply: 3)
1328. please help!!! with transformation - (reply: 10)
1329. sequencing primer: T7 or T7promoter? - (reply: 4)
1330. Unstable clone? - (reply: 1)
1331. Restriction enzymes - (reply: 3)
1332. Problems facing in cloning with pBI121 vector - (reply: 1)
1333. Failed Transformation of Tuner (DE3) cells - (reply: 1)
1334. Is my insert present??? - (reply: 17)
1335. Need help for restriction enzymes - (reply: 5)
1336. Heat inactivation/Gel purification or both? - Normally I purify from gel. Should I inactivate the enzymes? (reply: 2)
1337. Mini prep - (reply: 4)
1338. adding koazak sequence to primer - (reply: 1)
1339. DNA ligation - (reply: 2)
1340. adding C terminal tag to reverse primer - (reply: 1)
1341. glycerol stocks - (reply: 10)
1342. doubts in sequence submission to NCBI - (reply: 2)
1343. Concentration of DNA for Transformation - (reply: 1)
1344. Restriction digest - (reply: 7)
1345. Help! Double restriction digestion failed.... - (reply: 6)
1346. Kozak sequences.. - (reply: 1)
1347. Sequence management software (for Mac) - (reply: 5)
1348. ligation for 24 hrs in 4*C? - (reply: 3)
1349. Having problem establishing stable cell line...... - (reply: 5)
1350. Best method for Mutagenesis? - multisite mutagenesis (reply: 2)