|
1321. ligation problem with SalI degested vector and insert - (reply: 6)
1322. IRES1 x IRES2? - (reply: 5)
1323. Restriction site - efficiency of restriction (reply: 4)
1324. lentivirus vector problem--help help - (reply: 6)
1325. A problem with deleting URA3 from S. cerevisiae - (reply: 2)
1326. Can ligation mix be stored for 1 month in -20'C - Just curious if ligation mix can still be used for transformation (reply: 3)
1327. ligation - didn't got transformed colonies (reply: 1)
1328. What's this in my culture??? - Debris in liquid culture (reply: 3)
1329. assembling problem in genes - assembling problem in genes (reply: 7)
1330. tryptone soy broth - (reply: 3)
1331. no colonies - Cloning using pENTR vector, no colonies (reply: 2)
1332. Please help Cloning issues with smaI and pUNK1 vector - (reply: 2)
1333. ligation problem - (reply: 3)
1334. ligation question - three-point ligation (reply: 9)
1335. Problems cloning Hairpin vector - (reply: 3)
1336. request pIRES2-EGFP - (reply: 2)
1337. different digestion pattern for same clone - (reply: 4)
1338. Problem with DNA purification from agarose gel - (reply: 3)
1339. cloning trouble - (reply: 15)
1340. No band in the gel after ligation - (reply: 6)
1341. Troubleshooting Digest, ligation, transformation - (reply: 7)
1342. Ideal incubation time - (reply: 3)
1343. Competent DH5 alpha - (reply: 4)
1344. What is the difference between cotransformation and (single transformation to th - What is the difference between cotransformation and single transforma (reply: 2)
1345. Quick ? about Enzyme Buffers - (reply: 3)
1346. High conc. CaCl2 inhibits electroporation?? - (reply: 3)
1347. Inserts too small after transformation - transformation (reply: 3)
1348. glycerol stocks - storage (reply: 2)
1349. growth of colonies after ligation - (reply: 2)
1350. cDNA cloning problem - (reply: 1)
1322. IRES1 x IRES2? - (reply: 5)
1323. Restriction site - efficiency of restriction (reply: 4)
1324. lentivirus vector problem--help help - (reply: 6)
1325. A problem with deleting URA3 from S. cerevisiae - (reply: 2)
1326. Can ligation mix be stored for 1 month in -20'C - Just curious if ligation mix can still be used for transformation (reply: 3)
1327. ligation - didn't got transformed colonies (reply: 1)
1328. What's this in my culture??? - Debris in liquid culture (reply: 3)
1329. assembling problem in genes - assembling problem in genes (reply: 7)
1330. tryptone soy broth - (reply: 3)
1331. no colonies - Cloning using pENTR vector, no colonies (reply: 2)
1332. Please help Cloning issues with smaI and pUNK1 vector - (reply: 2)
1333. ligation problem - (reply: 3)
1334. ligation question - three-point ligation (reply: 9)
1335. Problems cloning Hairpin vector - (reply: 3)
1336. request pIRES2-EGFP - (reply: 2)
1337. different digestion pattern for same clone - (reply: 4)
1338. Problem with DNA purification from agarose gel - (reply: 3)
1339. cloning trouble - (reply: 15)
1340. No band in the gel after ligation - (reply: 6)
1341. Troubleshooting Digest, ligation, transformation - (reply: 7)
1342. Ideal incubation time - (reply: 3)
1343. Competent DH5 alpha - (reply: 4)
1344. What is the difference between cotransformation and (single transformation to th - What is the difference between cotransformation and single transforma (reply: 2)
1345. Quick ? about Enzyme Buffers - (reply: 3)
1346. High conc. CaCl2 inhibits electroporation?? - (reply: 3)
1347. Inserts too small after transformation - transformation (reply: 3)
1348. glycerol stocks - storage (reply: 2)
1349. growth of colonies after ligation - (reply: 2)
1350. cDNA cloning problem - (reply: 1)