|
1321. Cloning Problem - (reply: 3)
1322. AdEasy Vector System: Why PacI digestion? - (reply: 1)
1323. Can transormations be incubated at less than 37 degrees after heat shock? - What to do when the shaker is in use (reply: 4)
1324. cloning large insert in pGEM-T easy vector - Does TA cloning work for big inserts? (reply: 2)
1325. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
1326. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
1327. Restriction buffer composition - (reply: 2)
1328. same weird weird staph transformation results - any idea what is going on? (reply: 12)
1329. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
1330. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
1331. Yet another ligation problem - (reply: 4)
1332. Problems with Quick change lightning site directed mutagenesis kit(STRATAGENE) - (reply: 2)
1333. Promoter Cloning for Luciferase Assay - (reply: 3)
1334. Creating mutants - (reply: 3)
1335. cloning protocol - (reply: 1)
1336. Ligation Time and Temp - (reply: 4)
1337. Miniprep - (reply: 3)
1338. PCR and then ligation - (reply: 7)
1339. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
1340. 3 way ligation help needed - (reply: 10)
1341. Cloning and Expression Method Survey - Get some ideas from you all (reply: 1)
1342. Cloning woes - Running out of ideas! - (reply: 7)
1343. What fields of research other than microbiology work with bacteria and - (reply: 1)
1344. How to inoculate bacteria? - (reply: 10)
1345. What retrovirus vetor can have 7.5kb insert - (reply: 1)
1346. need help in my Fse1 restriction ezyme - (reply: 4)
1347. Insert problems with TA cloning - (reply: 1)
1348. Surface expression by tansfection of CHO - (reply: 3)
1349. please help!!! with transformation - (reply: 10)
1350. sequencing primer: T7 or T7promoter? - (reply: 4)
1322. AdEasy Vector System: Why PacI digestion? - (reply: 1)
1323. Can transormations be incubated at less than 37 degrees after heat shock? - What to do when the shaker is in use (reply: 4)
1324. cloning large insert in pGEM-T easy vector - Does TA cloning work for big inserts? (reply: 2)
1325. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
1326. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
1327. Restriction buffer composition - (reply: 2)
1328. same weird weird staph transformation results - any idea what is going on? (reply: 12)
1329. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
1330. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
1331. Yet another ligation problem - (reply: 4)
1332. Problems with Quick change lightning site directed mutagenesis kit(STRATAGENE) - (reply: 2)
1333. Promoter Cloning for Luciferase Assay - (reply: 3)
1334. Creating mutants - (reply: 3)
1335. cloning protocol - (reply: 1)
1336. Ligation Time and Temp - (reply: 4)
1337. Miniprep - (reply: 3)
1338. PCR and then ligation - (reply: 7)
1339. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
1340. 3 way ligation help needed - (reply: 10)
1341. Cloning and Expression Method Survey - Get some ideas from you all (reply: 1)
1342. Cloning woes - Running out of ideas! - (reply: 7)
1343. What fields of research other than microbiology work with bacteria and - (reply: 1)
1344. How to inoculate bacteria? - (reply: 10)
1345. What retrovirus vetor can have 7.5kb insert - (reply: 1)
1346. need help in my Fse1 restriction ezyme - (reply: 4)
1347. Insert problems with TA cloning - (reply: 1)
1348. Surface expression by tansfection of CHO - (reply: 3)
1349. please help!!! with transformation - (reply: 10)
1350. sequencing primer: T7 or T7promoter? - (reply: 4)