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1321. cloning large insert in pGEM-T easy vector - Does TA cloning work for big inserts? (reply: 2)
1322. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
1323. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
1324. Restriction buffer composition - (reply: 2)
1325. same weird weird staph transformation results - any idea what is going on? (reply: 12)
1326. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
1327. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
1328. Yet another ligation problem - (reply: 4)
1329. Problems with Quick change lightning site directed mutagenesis kit(STRATAGENE) - (reply: 2)
1330. Promoter Cloning for Luciferase Assay - (reply: 3)
1331. Creating mutants - (reply: 3)
1332. cloning protocol - (reply: 1)
1333. Ligation Time and Temp - (reply: 4)
1334. Miniprep - (reply: 3)
1335. PCR and then ligation - (reply: 7)
1336. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
1337. 3 way ligation help needed - (reply: 10)
1338. Cloning and Expression Method Survey - Get some ideas from you all (reply: 1)
1339. Cloning woes - Running out of ideas! - (reply: 7)
1340. What fields of research other than microbiology work with bacteria and - (reply: 1)
1341. How to inoculate bacteria? - (reply: 10)
1342. What retrovirus vetor can have 7.5kb insert - (reply: 1)
1343. need help in my Fse1 restriction ezyme - (reply: 4)
1344. Insert problems with TA cloning - (reply: 1)
1345. Surface expression by tansfection of CHO - (reply: 3)
1346. please help!!! with transformation - (reply: 10)
1347. sequencing primer: T7 or T7promoter? - (reply: 4)
1348. Unstable clone? - (reply: 1)
1349. Restriction enzymes - (reply: 3)
1350. Problems facing in cloning with pBI121 vector - (reply: 1)
1322. Help needed for ligation of large inserts into TOPO vector - (reply: 3)
1323. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
1324. Restriction buffer composition - (reply: 2)
1325. same weird weird staph transformation results - any idea what is going on? (reply: 12)
1326. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
1327. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
1328. Yet another ligation problem - (reply: 4)
1329. Problems with Quick change lightning site directed mutagenesis kit(STRATAGENE) - (reply: 2)
1330. Promoter Cloning for Luciferase Assay - (reply: 3)
1331. Creating mutants - (reply: 3)
1332. cloning protocol - (reply: 1)
1333. Ligation Time and Temp - (reply: 4)
1334. Miniprep - (reply: 3)
1335. PCR and then ligation - (reply: 7)
1336. cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
1337. 3 way ligation help needed - (reply: 10)
1338. Cloning and Expression Method Survey - Get some ideas from you all (reply: 1)
1339. Cloning woes - Running out of ideas! - (reply: 7)
1340. What fields of research other than microbiology work with bacteria and - (reply: 1)
1341. How to inoculate bacteria? - (reply: 10)
1342. What retrovirus vetor can have 7.5kb insert - (reply: 1)
1343. need help in my Fse1 restriction ezyme - (reply: 4)
1344. Insert problems with TA cloning - (reply: 1)
1345. Surface expression by tansfection of CHO - (reply: 3)
1346. please help!!! with transformation - (reply: 10)
1347. sequencing primer: T7 or T7promoter? - (reply: 4)
1348. Unstable clone? - (reply: 1)
1349. Restriction enzymes - (reply: 3)
1350. Problems facing in cloning with pBI121 vector - (reply: 1)