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Molecular-Cloning
1321.
Lb-kan plates life - How long can they be used?
(reply: 2)
1322.
LR reaction - Cloning into a expression vector
(reply: 1)
1323.
TOPO-Transformation Problem -
(reply: 4)
1324.
Checking Plasmid Expression -
(reply: 1)
1325.
designing primers for genes not sequenced yet -
(reply: 2)
1326.
trouble with plasmid transformation in BL21DE3 E. coli cells -
(reply: 9)
1327.
Cloning Problem -
(reply: 3)
1328.
AdEasy Vector System: Why PacI digestion? -
(reply: 1)
1329.
Can transormations be incubated at less than 37 degrees after heat shock? - What to do when the shaker is in use
(reply: 4)
1330.
cloning large insert in pGEM-T easy vector - Does TA cloning work for big inserts?
(reply: 2)
1331.
Help needed for ligation of large inserts into TOPO vector -
(reply: 3)
1332.
What is the type of replicon from pGC blue plasmid? - Cloning
(reply: 2)
1333.
Restriction buffer composition -
(reply: 2)
1334.
same weird weird staph transformation results - any idea what is going on?
(reply: 12)
1335.
overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests?
(reply: 12)
1336.
Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq?
(reply: 7)
1337.
Yet another ligation problem -
(reply: 4)
1338.
Problems with Quick change lightning site directed mutagenesis kit(STRATAGENE) -
(reply: 2)
1339.
Promoter Cloning for Luciferase Assay -
(reply: 3)
1340.
Creating mutants -
(reply: 3)
1341.
cloning protocol -
(reply: 1)
1342.
Ligation Time and Temp -
(reply: 4)
1343.
Miniprep -
(reply: 3)
1344.
PCR and then ligation -
(reply: 7)
1345.
cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83
(reply: 1)
1346.
3 way ligation help needed -
(reply: 10)
1347.
Cloning and Expression Method Survey - Get some ideas from you all
(reply: 1)
1348.
Cloning woes - Running out of ideas! -
(reply: 7)
1349.
What fields of research other than microbiology work with bacteria and -
(reply: 1)
1350.
How to inoculate bacteria? -
(reply: 10)
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