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Top : New Forum Archives (2009-): : Molecular-Cloning
1321. from where can i get the P1 phage - (reply: 1)
1322. multi way ligation or long range pcr? - (reply: 1)
1323. Making of alkaline lysis solution - Funny white colouratio of lysis solution (reply: 4)
1324. list of sequence to be added before RE site - (reply: 3)
1325. plasmid, BAC, or cosmid? - (reply: 3)
1326. Wierd trouble - dunno where my insert is?? (reply: 3)
1327. Large Molecular weight DNA Extarction - (reply: 7)
1328. Ligation of digested plasmid to hairpin oligo - (reply: 5)
1329. Nucleospin Extract II kit also for plasmids? - (reply: 5)
1330. Difficulty cloning vector with multiple Lox sites - (reply: 2)
1331. Preferentially wrong orientation of inserts in ligation reactions - ive never seen this before... (reply: 2)
1332. Is it pointless to check orientation if non-compatible ends are used? - (reply: 2)
1333. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
1334. Vector sequence included in (PCR) amplified insert - (reply: 2)
1335. COMPETENT CELL PREPARATION - (reply: 6)
1336. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
1337. Accidentally deleted enzyme from Vector NTI - how to restore? - (reply: 1)
1338. What Carbenecillin concentration does everyone normally use??? - (reply: 3)
1339. T A cloning problems - (reply: 3)
1340. Gel electrophoresis and purifying dna - rnase treatment or not? (reply: 6)
1341. Maximum recovery time after electroporation - (reply: 2)
1342. pCLBABE puro retriviral vector - (reply: 1)
1343. Random library using TOPO vector - random genomic DNA ligated into TOPO, false inserts? (reply: 3)
1344. Blunt-end cloning problem - (reply: 6)
1345. Supercoiling blocking restriction site? - (reply: 6)
1346. Chooseing the best sequence for clonning - How to choose the best sequence for clonning a cDNA (reply: 6)
1347. problem in gene cloning - (reply: 4)
1348. Odd point mutations - (reply: 23)
1349. Plasmid linearization by PCR - (reply: 4)
1350. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)